Effect of different Streptomyces strains on pine wood as seen by Py–GC/MS

Abstract

Pyrolysis/gas chromatography/mass spectrometry was used to elucidate the ability of streptomycetes to modify pine wood under solidstate fermentation. The pyrograms of sound and decayed wood showed the typical pyrolysis products derived from carbohydrates and lignin. In general, the relative abundance of the former was lower in treated woods than in sound wood. Several major peaks released from guaiacyl units of lignin were also revealed. Although some differences could be observed in the relative abundance of the released guaiacyl units from lignin between sound and treated woods, a clear decrease in the lignin-derived products by the action of the microorganisms was not observed. Nevertheless, an increase in the relative abundance of the most of guaiacyl units with a higher oxidation degree was mainly detected in woods treated by the strains Streptomyces UAH 7, Streptomyces anulatus, Streptomyces UAH 48, Streptomyces avermitilis and Streptomyces badius. These results suggest an oxidative action of these strains on the lignin of pine wood, which was corroborated by a decrease in the (aldehyde/ ketone + acid) ratio. In addition, the breakdown of the alkyl chain linkages by the strain Streptomyces UAH 7 could be also observed. Analytical pyrolysis allow to demonstrate for first time the oxidative attack of selected Streptomyces strains on lignin moiety of pine wood, which is an important requirement for biomechanical pulping purposes. #

Evolution of Composting Process in Maize Biomass Revealed by Analytical Pyrolysis (Py-GC/MS) and Pyrolysis Compound Specific Isotope Analysis (Py-CSIA)

“…An increase in the sum of the relative abundance of non-oxidized alkyl phenols points to the selective preservation of some constituents of the lignin macromolecule in the compost pile. An increase in the relative abundance of higher oxidation degree G-compounds (vanillin, acetovanillone, and vanillic acid methyl ester; guaiacylacetone, propiovanillone) suggest an oxidative action resulting from the decomposition process of compost [54]. This may come from either oxidation or oxidation followed by cleavage of the C3 alkyl chain from G units at the Cα and Cβ position.…”

Direct Detection of Black Carbon in Soils by Py‐GC/MS, Carbon‐13 NMR Spectroscopy and Thermogravimetric Techniques

“…The double shot pyrolyzer allows thermal desorption of samples (at subpyrolysis temperature) before pyrolysis, and so allows the sequential examination of the products released by thermal desorption and by thermal cracking from the same sample. This technique has been used recently for the analysis of a wide spectrum of complex carbonaceous matrices including fossil OM, polymers, algal, and higher plant materials (Yoshioka and Ishiwatari, 2002;Herrera et al, 2003;Lee et al, 2005;Camean et al, 2005;Arias et al, 2005).…”

Double-shot pyrolysis of the non-hydrolysable organic fraction isolated from a sandy forest soil (Landes de Gascogne, South-West France)

“…The non-hydrolysable fraction was examined by ''double-shot'' pyrolysis. This recent method was used in the last few years for the analysis of fossil organic matter, polymers, algal and higher plant materials [7][8][9][10][11]. The technique allows the sequential examination of the products released by thermal desorption and by thermal cracking from the same sample.…”