Effect of dihydrotestosterone on the upregulation of inflammatory cytokines in cultured sebocytes

Lee, Weon Ju; Jung, H.; Geun, Seong; Kim, Byung Soo; Lee, Seok Jong; Kim, Do Won; Kim, Moon Kyu; Kim, Jung Chul

doi:10.1007/s00403-009-1019-6

Cited by 41 publications

(25 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…[45] In general, the effects of vitamin D are not only important in calcium homeostasis, but also in immune regulation, cell growth, and cell differentiation. Binding of vitamin D to vitamin D receptor stimulates the proliferation of sebocytes, and inhibits the differentiation and lipid synthesis of sebocytes.…”

Section: Discussionmentioning

confidence: 99%

“…Based on previous studies that substance P and dihydrotestosterone might be involved in the production of inflammatory cytokines in sebaceous glands in acne,[45] we conducted this study to define the effect of vitamin D on the expression of inflammatory biomarkers from sebocytes, such as interleukin-1β (IL-1β), IL-6, IL-8, and tumor necrosis factor-α (TNF-α), and matrix metalloproteinase-1 (MMP-1), MMP-3, and MMP-9.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Expression of inflammatory biomarkers from cultured sebocytes was influenced by treatment with vitamin D

et al. 2013

Self Cite

View full text Add to dashboard Cite

Background:Inflammatory cytokines are the key factor in the pathophysiology of acne. It is well known that keratinocytes synthesize many kinds of inflammatory cytokines. In addition, it is reported that inflammatory cytokines are also expressed from sebocytes, which originate from the same stem cells with keratinocytes.Aim:To clarify changes in the expression of inflammatory biomarkers from cultured sebocytes after treatment with vitamin D.Materials and Methods:Reverse transcription-polymerase chain reaction (RT-PCR) was done to measure changes in the expression of inflammatory biomarkers, including interleukin-1β (IL-1β), IL-6, IL-8, and tumor necrosis factor-α (TNF-α), and several subtypes of matrix metalloproteinases (MMPs) after treatment of a group of cultured sebocytes with vitamin D. Vitamin D receptor (VDR) small interfering RNA (siRNA) was added in the other group of cultured sebocytes to assure the role of vitamin D on the expression of inflammatory biomarkers. Enzyme-linked immunosorbent assay (ELISA) was also performed in the vitamin D-treated sebocytes.Results:Cultured sebocytes showed non-significant changes in the gene expression of inflammatory biomarkers after treatment with vitamin D. In cultured sebocytes treated with a VDR siRNA, the expression of inflammatory biomarkers was not blocked after treatment with vitamin D. ELISA showed a significant decrease in the expression of IL-6, IL-8, and MMP-9, but a significant increase in the expression of MMP-1 and MMP-3, after treatment with vitamin D (10-6 M).Conclusion:Expression of inflammatory biomarkers is influenced by treatment with vitamin D in cultured sebocytes, but not through VDR.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Expression of inflammatory biomarkers from cultured sebocytes was influenced by treatment with vitamin D

et al. 2013

Self Cite

View full text Add to dashboard Cite

show abstract

“…Involvement of SP, the CRH signaling system and the MC1 receptor in acne development was also suggested [226–229]. The androgen/AR complex does not only affect sebaceous gland activity, but also its inflammation, by augmenting the inflammatory responses of neutrophils and macrophages [230, 231]. …”

Section: Role Of Local Steroidogenic Pathways In Inflammatory Disomentioning

confidence: 99%

Steroidogenesis in the skin: Implications for local immune functions

Slominski

Zbytek

Nikolakis

et al. 2013

The Journal of Steroid Biochemistry and Molecular Biology

329

318

View full text Add to dashboard Cite

The skin has developed a hierarchy of systems that encompasses the skin immune and local steroidogenic activities in order to protect the body against the external environment and biological factors and to maintain local homeostasis. Most recently it has been established that skin cells contain the entire biochemical apparatus necessary for production of glucocorticoids, androgens and estrogens either from precursors of systemic origin or, alternatively, through the conversion of cholesterol to pregnenolone and its subsequent transformation to biologically active steroids. Examples of these products are corticosterone, cortisol, testosterone, dihydrotesterone and estradiol. Their local production can be regulated by locally produced corticotropin releasing hormone (CRH), adrenocorticotropic hormone (ACTH) or cytokines. Furthermore the production of glucocorticoids is affected by ultraviolet B radiation. The level of production and nature of the final steroid products are dependent on the cell type or cutaneous compartment, e.g., epidermis, dermis, adnexal structures or adipose tissue. Locally produced glucocorticoids, androgens and estrogens affect functions of the epidermis and adnexal structures as well as local immune activity. Malfunction of these steroidogenic activities can lead to inflammatory disorders or autoimmune diseases. The cutaneous steroidogenic system can also have systemic effects, which are emphasized by significant skin contribution to circulating androgens and/or estrogens. Furthermore, local activity of CYP11A1 can produce novel 7 -steroids and secosteroids that are biologically active. Therefore, modulation of local steroidogenic activity may serve as a new therapeutic approach for treatment of inflammatory disorders, autoimmune processes or other skin disorders. In conclusion, the skin can be defined as an independent steroidogenic organ, whose activity can affect its functions and the development of local or systemic inflammatory or autoimmune diseases.

show abstract

“…To modulate acne development from the aspect of sebaceous gland function, there have been several efforts to define possible substances to influence the function of sebocytes. So far, substance‐P, dihydrotestosterone and vitamin D have been proved to affect the expression of inflammatory cytokines from cultured sebocytes . Furthermore, the relation between UV‐B irradiation and function of the sebaceous gland has been widely studied.…”

Section: Introductionmentioning

confidence: 99%

Ultraviolet B irradiation increases the expression of inflammatory cytokines in cultured sebocytes

Lee

Park

Sohn

et al. 2013

The Journal of Dermatology

Self Cite

View full text Add to dashboard Cite

Sebaceous gland hyperplasia and increased sebum secretion after irradiation of ultraviolet (UV)-B has been widely accepted. This study was performed to clarify expression of inflammatory cytokines after irradiating UV-B in cultured sebocytes. Reverse transcription polymerase chain reaction was performed to measure gene expression of inflammatory cytokines, including interleukin (IL)-1β, IL-6, IL-8 and tumor necrosis factor-α, in cultured sebocytes after exposure to 40 and 70 mJ/cm(2) UV-B. Protein expression of inflammatory cytokines and lipid production in cultured sebocytes after exposure to UV-B were measured using enzyme-linked immunoassay and lipid analysis kit. The expression of inflammatory cytokines, especially IL-1β and IL-8, was significantly increased in cultured sebocytes after treatment with UV-B. Many more studies on the effect of UV-B on sebaceous glands should be performed to reveal the pathogenic mechanism of acne.

show abstract

Effect of dihydrotestosterone on the upregulation of inflammatory cytokines in cultured sebocytes

Cited by 41 publications

References 17 publications

Expression of inflammatory biomarkers from cultured sebocytes was influenced by treatment with vitamin D

Expression of inflammatory biomarkers from cultured sebocytes was influenced by treatment with vitamin D

Steroidogenesis in the skin: Implications for local immune functions

Ultraviolet B irradiation increases the expression of inflammatory cytokines in cultured sebocytes

Contact Info

Product

Resources

About