Effect of dill tablet (Anethum graveolens L) on antioxidant status and biochemical factors on carbon tetrachloride-induced liver damage on rat

Abstract

Background:Liver damage induced by carbon tetrachloride (CCl4) has been presented as an experimental model for research in hepatoprotective effects of natural product. A commercial medicine prepared from Anethum graveolens L (dill) is being used as dill tablet (DT) as a hypolipidemic agent. This experiment aimed to investigate the protective effect of DT against hepatic damage.Materials and Methods:Male Wistar rats were randomly divided into four groups (n = 6) as following for a 10 days experiments. (1) Normal animals; (2) normal animals +CCl4 1 ml/kg (1:1 of CCl4 in olive oil, by gastric tube); (3) CCl4 treated animals +100 mg DT/kg; (4) CCl4 treated animals +300 mg DT/kg. After 10 days of treatment, biochemical factors were measured; also antioxidant tests such as thiol group, malondialdehyde (MDA), total antioxidant capacity (TAC), and catalase (CAT) activity in the liver samples were carried out.Results:In dill treated animals, a significant decrease in liver enzymes lactate dehydrogenase, alkaline phosphatase, aspartate transaminase, alanine transaminase, γ-glutamyl transferase, total bilirubin, direct bilirubin, as well as triglyceride, total cholesterol (P < 0.05) were observed. Total protein and albumin concentrations were significantly increased in dill treated groups (P < 0.05). Furthermore, treatment with dill declined liver cholesterol, triglyceride, MDA, and increased TAC and CAT activity compared with untreated group (P < 0.05).Conclusion:Dill displayed a potential hepatoprotective effect against CCl4-induced liver damage based on both biochemical markers and antioxidant status.

Hepatoprotective potential of Fagonia olivieri DC. against acetaminophen induced toxicity in rat

“…Co-treatment with F. olivieri repaired the liver architecture and protected the hepatic tissue from degenerative and fatty alterations, by averting the toxic chemical reaction, oxidative stress, lipid peroxidation, molecular changes in the liver tissues, micro and macro vesicular fatty changes ultimately leading to necrosis. These findings are supported by the previous hepatoprotective abilities of plant extracts [57, 58]. …”

The Role ofAnethum graveolensL. (Dill) in the Management of Diabetes

“…Currently, many studies have established these properties; also, AG is recently known as anticancer, antimicrobial, antigastric irritation, anti-inflammatory, and antioxidant agent. In this respect, dill is produced as a hypolipidemic drug ( Anethum tablet) in Iran which consists of Anethum graveolens (68%), Cichorium intybus (5%), Fumaria parviflora (5%), and lime (Citrus aurantifolia) (4%) [81]. …”

Glucagon like Peptide-1(GLP-1) a Novel Therapeutic Strategy in Non-Alcoholic Fatty Liver Disease (NAFLD)

“…On the other hand, pharmaceutical therapies such as insulin sensitizers, renin-angiotensin system blockers, lipid reducing drugs, vitamin E and natural antioxidants, have been used in clinical trials (7). For instance, herbal medicine and natural products can prevent or manage NAFLD or liver injury by various mechanisms, such as reducing liver lipid accumulation via reducing sterol regulatory element-binding protein 1c (SREBP-1c) expression, elevating β-fatty acid (FA) oxidation by increasing peroxisome proliferator activated receptor α (PPARα) expression, reducing IR, alleviating oxidative stress through increased antioxidant enzymes activity and suppressing inflammatory factors (8)(9)(10)(11)(12)(13)(14)(15). Although natural products or various vitamins and some chemical drugs are used in liver damage, recent therapies for NAFLD show few positive outcomes and some adverse effects.…”