Effect of Direct-Current Electric Field on Enzymatic Activity and the Concentration of Laccase

Abstract

This work investigates the effect of directcurrent electric field on the extracellular enzymatic activity, concentration and other experimental parameters of laccase from Trametes versicolor. The results showed that laccase could significantly contribute to the change of pH at the end of graphite electrode. In addition, it increased the electrical conductivity of the water. In the experiment, the optimum pH and catalytic pH range for laccase activity were 3.0 and pH 2.5-4.0. The application of 6 V direct current showed significant effects on the laccase enzyme activity. The activity of laccase was enhanced in the anodic region, but at the same time was strongly inhibited at the cathode. The electric charge characteristics of laccase were changed when exposed to electric field, and some laccases molecules moved to the anode, which produced a slight migration phenomenon. This study is the basis of combination of laccase and electrical technology, at the same time, providing a new direction of enhancing laccase activity. Compared to immobilization, using electric field is simple, no chemical additives, and great potential.

Facile Immobilization of Enzyme via Co-Electrospinning: A Simple Method for Enhancing Enzyme Reusability and Monitoring an Activity-Based Organic Semiconductor

“…Moreover, a similar study reported that a direct electric current exhibited less effect on the laccase enzyme, and the laccase and electric field exhibited a better response to practical environment changes. 28…”

Biodegradation of phenol by pleurotus laccase in the directcurrent electric field

“…Owing to the hydroxyl group in its structure, phenol exhibits weak acidity. However, it has been demonstrated that laccase exhibits higher catalytic activity in acidic environments [4]. This property makes laccase a good candidate for promoting the catalytic degradation of phenol.…”

“…Hence, laccase is considered an environmentally friendly fermenting agent, and its role in biodegradation catalysis has been extensively studied [3]. Previously, the effects of electric fields on white rot fungi and laccase, as well as the effects of electric fields on the biodegradation of phenolic compounds were studied [4]. Nevertheless, few studies have reported the effects of electric fields on phenol biodegradation catalyzed by laccase.…”

Impact of electrocoagulation on membrane filtration resistance under different proportions of industrial wastewater

“…13 Bani-Melhem et al found that integrating the electrocoagulation process with a membrane bioreactor was an effective method for wastewater treatment and for improving membrane filtration. 14 Some researchers studied that external electric fields were capable of enhancing the activities of enzymes and anammox bacteria, 15,16 Additionally, previous studies indicated that a bio-electrocoagulation system with Fe-C electrodes was able to increase the biomass and system performance. 17,18 These results revealed that electrocoagulation technology was able to increase membrane filtration performance as well as activated sludge performance.…”