Effect of disinfectants containing glutaraldehyde on bonding of a tri‐n‐butylborane initiated resin to dentine

Abstract

The purpose of this study was to evaluate the effects of disinfectants on the bond strength of resin to dentine. The surface of bovine dentine was exposed to formaldehyde (FA) aqueous solutions, glutaraldehyde (GA) aqueous solutions, 2-hydroxyethyl methacrylate aqueous solutions (HEMA), a commercially available dentine primer (Gluma CPS desensitizer, GLUMA), isotonic sodium chloride solution (IS), and distilled water (DW), and placed in a humidor (HU) at 37 degrees C, or non-stored (baseline). All dentine surfaces were conditioned with a 10% citric acid and 3% ferric chloride solution (10-3 liquid), and then bonded to an acrylic rod with a self-curing adhesive resin (Super-Bond C&B). The mean tensile bond strengths determined 24 h after bonding were compared by analysis of variance (ANOVA) and Fisher's protected LSD test (n=5, P < or = 0.05). The exposure of dentine to IS, DW and HU for both 48 and 168 h resulted in a decrease in bond strength when compared with the baseline. The highest bond strengths after 168 h of exposure were obtained with 5% GA, 10% HEMA, and GLUMA, the values of which were equivalent to baseline and were significantly higher than that of FA. It is concluded that disinfectant pre-treatment with 5% GA or GLUMA stabilizes the bonding of tri-n-butylborane (TBB) initiated luting agent to bovine dentine conditioned with 10-3 liquid.

Review of methyl methacrylate (MMA)/tributylborane (TBB)-initiated resin adhesive to dentin

“…A desensitizer containing 5% glutaraldehyde and 36% HEMA was beneficial for the MMA-TBB bonding system 29,75) . Formaldehyde did not markedly improve dentin bond strength when compared with glutaraldehyde 27) . As for cresol, eugenol, and phenol camphor, they negatively affected the bond strength of MMA-TBB bonding system to dentin, suggesting polymerization inhibition 26,76,77) .…”

“…Comparison of adherend materials between Groups 1-4 and Group 5, and between Group 7 and Group 8, indicated that adhesive bonding of metal to dentin was weaker than that of acrylic material to dentin 8,[25][26][27][28][29][30] . Tensile testing using dumbbell-shaped specimens revealed that conditioning with 10-3 conditioner for 30 s resulted in significantly lower bond strength than conditioning for 10 s only 31,32) .…”

“…Another suggested mechanism was that glutaraldehyde promoted the graft polymerization of MMA onto dentin collagen 74) . Although 10% or 25% glutaraldehyde reduced bond strength, 5% glutaraldehyde produced a positive effect for the 10-3/4-META/MMA-TBB bonding system 27,72) . A desensitizer containing 5% glutaraldehyde and 36% HEMA was beneficial for the MMA-TBB bonding system 29,75) .…”

“…Masuhara's original work made a significant contribution to dentin bonding technology, which should be shared internationally. This report reviews the features of MMA/TBB resin 8,[25][26][27] . When 10-3 conditioner was used in conjunction with 4-META/MMA-TBB resin (10-3/4-META/MMA-TBB system), bonding effectiveness might be hindered by technique sensitivity.…”

Sterilization of extracted human teeth: A comparative analysis

“…23 Baba et al evaluated the effects of disinfectants on the bond strength of resin to dentine and found that highest bond strengths was obtained with 5% glutaraldehyde after 168 h of exposure. 24 Our study is a pilot endeavor. Further studies on a larger sample and including more sterilizing agents are required to be undertaken to substantiate the more effective method of sterilization.…”