Effect of disopyramide on isolated aortic ring of the rat

Moura, Roberto Soares de; Lima, J S

doi:10.1111/j.1476-5381.1983.tb08803.x

Cited by 5 publications

(1 citation statement)

References 9 publications

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“…The rings were suspended between two stainless steel stirrups in an organ bath filled with 10 ml Krebs solution at 37°C. The composition of Krebs solution was (in mmol): NaCl, 118; KCl, 4.7; CaCl 2 , 2.5; MgSO 4 , 1.18; KH 2 PO4, 1.08; NaHCO 3 , 25; Glucose, 11; and bubbled with 95%O 2 +5% CO 2 (PH 7.35–7.45) [22] . Organ bath was changed with Krebs solution every 15 mins.…”

Section: Methodsmentioning

confidence: 99%

Tetramethylpyrazine Ameliorates High Glucose-Induced Endothelial Dysfunction by Increasing Mitochondrial Biogenesis

Xia

et al. 2014

PLoS ONE

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Tetramethylpyrazine (TMP) is an active compound isolated from a Chinese herbal prescription that is widely used in traditional Chinese medicine for the treatment of inflammatory and cardiovascular diseases. We have previously reported that TMP acts as a potent antioxidant protecting endothelial cells against high glucose-induced damages. However, the molecular mechanism responsible for the antioxidant effect of TMP remains to be elucidated. In this study, we show that TMP increases nitric oxide production in endothelial cells and promotes endothelium-dependent relaxation in rate aortic rings. The antioxidant effect of TMP appears attributable to its ability to activate the mitochondrial biogenesis, as reflected in an up-regulation of complex III and amelioration of mitochondrial membrane potential. Furthermore, TMP is able to reverse high glucose-induced suppression of SIRT1 and the biogenesis-related factors, including PGC-1α, NRF1 and TFAM, suggesting a new molecular mechanism underlying the protective effect of TMP on the endothelium.

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Section: Methodsmentioning

confidence: 99%