Effect of distigmine on the contractile response of guinea pig urinary bladder to electrical field stimulation

Obara, Kazuo; Kobayashi, Yurina; Chino, Daisuke; Tanaka, Yoshio

doi:10.1016/j.ejphar.2017.05.031

Cited by 4 publications

(1 citation statement)

References 21 publications

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“…In human UBSM, distigmine may enhance contractile responses to parasympathetic nerve excitement induced by electrical field stimulation (EFS). 43) However, it is unknown which endogenous parasympathetic neurotransmitters cause this potentiation. Guinea pig UBSM contractile responses to EFS consisted of a contractile component sensitive to the muscarinic receptor antagonist atropine and another affected by the purinergic receptor-desensitizing agent α,β-methylene ATP (α,β-mATP).…”

Section: Effects Of Distigmine On Ubsm Contrac-tile Responses Elicitementioning

confidence: 99%

Effects of Distigmine on the Mechanical Activity of Urinary Bladder Smooth Muscle

Obara

Tanaka

2019

Biological & Pharmaceutical Bulletin

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Distigmine bromide (distigmine) is a reversible carbamate cholinesterase (ChE) inhibitor. Its principle clinical application is in the treatment of myasthenia gravis. Distigmine is also used as a remedy for dysuria and glaucoma. Its effectiveness in the management of dysuria has been demonstrated in several clinical reports. Distigmine may improve (enhance) urinary bladder smooth muscle (UBSM) contraction during micturition by inhibiting acetylcholine (ACh) decomposition. However, the pharmacological effects of distigmine on UBSM have not been adequately studied so far. In this review article, we summarize the reported effects of distigmine on the contractile responses elicited by exogenous and endogenous ACh in isolated UBSM preparations. We also discuss the effects of distigmine on the UBSM basal tone and the contractile response of UBSM to ATP, which is co-released with ACh from parasympathetic nerve terminals.

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Section: Effects Of Distigmine On Ubsm Contrac-tile Responses Elicitementioning

confidence: 99%

Effects of Distigmine on the Mechanical Activity of Urinary Bladder Smooth Muscle

Obara

Tanaka

2019

Biological & Pharmaceutical Bulletin

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Pharmacological study on the enhancing effects of U46619 on guinea pig urinary bladder smooth muscle contraction induced by acetylcholine and α,β-methylene ATP and the possible involvement of protein kinase C

Ou,

Komura,

Hojo

et al. 2023

Journal of Pharmacological Sciences

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Platelet-activating factor (PAF) strongly enhances contractile mechanical activities in guinea pig and mouse urinary bladder

Liu

Kaneko

Yoshioka

et al. 2022

Sci Rep

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In this study, we investigated the effects of platelet-activating factor (PAF) on the basal tone and spontaneous contractile activities of guinea pig (GP) and mouse urinary bladder (UB) smooth muscle (UBSM) tissues to determine whether PAF could induce UBSM tissue contraction. In addition, we examined the mRNA expression of the PAF receptor, PAF-synthesizing enzyme (lysophosphatidylcholine acyltransferase, LPCAT), and PAF-degrading enzyme (PAF acetylhydrolase, PAF-AH) in GP and mouse UB tissues using RT-qPCR. PAF (10−9–10−6 M) strongly enhanced the basal tone and spontaneous contractile activities (amplitude and frequency) of GP and mouse UBSM tissues in a concentration-dependent manner. The enhancing effects of PAF (10−6 M) on both GP and mouse UBSM contractile activities were strongly suppressed by pretreatment with apafant (a PAF receptor antagonist, GP: 10−5 M; mouse: 3 × 10−5 M). The PAF receptor (Ptafr), LPCAT (Lpcat1, Lpcat2), and PAF-AH (Pafah1b3, Pafah2) mRNAs were detected in GP and mouse UB tissues. These findings reveal that PAF strongly enhances the contractile mechanical activities of UBSM tissues through its receptor and suggest that the PAF-synthesizing and -degrading system exists in UBSM tissues. PAF may serve as both an endogenous UBSM constrictor and an endogenous mediator leading to detrusor overactivity.

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Effect of distigmine on the contractile response of guinea pig urinary bladder to electrical field stimulation

Cited by 4 publications

References 21 publications

Effects of Distigmine on the Mechanical Activity of Urinary Bladder Smooth Muscle

Effects of Distigmine on the Mechanical Activity of Urinary Bladder Smooth Muscle

Pharmacological study on the enhancing effects of U46619 on guinea pig urinary bladder smooth muscle contraction induced by acetylcholine and α,β-methylene ATP and the possible involvement of protein kinase C

Platelet-activating factor (PAF) strongly enhances contractile mechanical activities in guinea pig and mouse urinary bladder

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