2009
DOI: 10.1128/aac.00471-08
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Effect of DNase and Antibiotics on Biofilm Characteristics

Abstract: The role of extracellular DNA in the maintenance of biofilms formed by gram-positive and gram-negative bacteria was studied. This study evaluated all the bacterial strains that were tested for the presence of extracellular DNA with an average size of 30 kb in the matrix. Our results indicate changes in community biomass, architecture, morphology, and the numbers of CFU in the presence of DNase. This effect seems to be common to biofilms established by various unrelated gram-positive and gram-negative bacteria.… Show more

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Cited by 338 publications
(267 citation statements)
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“…The use of vancomycin alone was ineffective against biofilm cells of the isolates studied, whereas NAC demonstrated a significant inhibitory action expressed by reductions of viability of circa of 4-5 log 10 CFU/mL (Figures 1 and 2). As described by several authors, antibiotics at MIC concentration cause no killing effect on bacteria when growing in biofilm communities (Tetz et al, 2009). Our results are in accordance with the previous researchers since NAC and vancomycin alone or in combination both at MIC concentration caused no killing effect on staphylococci cells, showing a viable cells reduction of less than 3 log 10 .…”
Section: Discussionsupporting
confidence: 83%
“…The use of vancomycin alone was ineffective against biofilm cells of the isolates studied, whereas NAC demonstrated a significant inhibitory action expressed by reductions of viability of circa of 4-5 log 10 CFU/mL (Figures 1 and 2). As described by several authors, antibiotics at MIC concentration cause no killing effect on bacteria when growing in biofilm communities (Tetz et al, 2009). Our results are in accordance with the previous researchers since NAC and vancomycin alone or in combination both at MIC concentration caused no killing effect on staphylococci cells, showing a viable cells reduction of less than 3 log 10 .…”
Section: Discussionsupporting
confidence: 83%
“…As described by several authors, antibiotics at concentrations of 10 2 to 10 4 times the MIC cause no killing effect on bacteria in biofilm communities [21]. Taking this into consideration, and with the aim of testing higher concentrations than MIC, we determined the susceptibility of S. epidermidis biofilms to daptomycin, linezolid, and rifampicin at the respective peak serum concentrations, since such concentration is the highest level of drug that can be obtained in the blood, usually following the administration of multiple doses.…”
Section: Resultsmentioning
confidence: 99%
“…Biochemical characterization of the biofilm matrix was investigated by the addition of sodium meta-periodate, proteinase K and DNase I, which are reagents used to determine the composition of biofilm matrices (Tetz et al 2009;Gjermansen et al 2010). …”
Section: Resultsmentioning
confidence: 99%