Effect of docetaxel‑loaded lipid microbubble in combination with ultrasound‑triggered microbubble destruction on the growth of a gastric cancer cell line

Abstract

Although gastric cancer therapy has been improved, more efficient treatment strategies still need to be developed. In the present study, a docetaxel (DOC)-loaded lipid microbubble (DLLD) was prepared and the effect of DLLD combined with ultrasound-triggered microbubble destruction (UTMD) on the growth of a gastric cancer cell line was investigated. The following four groups were included in the present study: Control, DOC, DLLD and DLLD plus UTMD. The determined entrapment efficiency of DLLD is 76±3.5%. The present study demonstrated that treatment with DLLD plus UTMD could significantly inhibit the growth of the cultured gastric cancer cell line BGC-823 via arresting the cell cycle in G 2 /M phase, inhibiting cell DNA synthesis, promoting cell apoptosis and disrupting mitochondrial membrane potential, as compared with treatment with DOC or DLLD alone. Furthermore, the expression of p53, p21 and Bax were identified to be significantly upregulated, while that of Bcl-2 was significantly downregulated in the DLLD plus UTMD group. Therefore, treatment with DLLD plus UTMD was more efficient in inhibiting cell proliferation and inducing cell apoptosis in the gastric cancer cell line, when compared with treatment with DOC or DLLD alone, suggesting that DLLD plus UTMD could serve as a promising strategy for the treatment of gastric cancer.

Intelligent Algorithm-Based CT Imaging for Evaluation of Efficacy of Docetaxel Combined with Fluorouracil on Patients with Gastric Cancer

“…Currently, chemotherapy is the main treatment method to maximize the survival period and improve the quality of life of patients. Some anticancer drugs, such as paclitaxel, gemcitabine, and irinotecan, if combined with uracil, have an effective rate of 33%–75% [ 11 ]. Docetaxel is a kind of paclitaxel drug, which inhibits the mitosis and proliferation of tumor cells by promoting the assembly of tubulin into stable microtubules [ 12 ].…”

Novel Drug Delivery Systems as an Emerging Platform for Stomach Cancer Therapy

“…B. Lai et al evaluated the effect of docetaxel-loaded lipid microbubble (DLLD) in combination with ultrasound-triggered microbubble destruction (UTMD) on the growth of a gastric cancer cell line [169]. It was observed that the combined treatment effectively inhibits the growth of a gastric cell line, through cell-cycle arrest, promotion of apoptosis, etc.…”