Effect of DOGO phreatic water containing sulphur on Atopic Dermatitis in NC/Nga mouse

Abstract

Objectives : The purpose of this study is to investigate the effects of DOGO phreatic water containing sulphur on Atopic Dermatitis in NC/Nga mouse. Methods : We made DOGO phreatic water removed sulphur using Twin Alternating Sulfate Eater. After making atopic dermatitis caused by sensitizing NC/Nga mouse to DNCB(dinitrochlorobenzene), we made mouse swim in tanks each filled with distilled water, tap water, DOGO phreatic water(contain sulphur), DOGO phreatic water(remove sulphur) for 30minutes everyday. 3weeks later, we analyzed skin clinical score, total IgE levels(by ELISA), WBC differential counting(Neutrophils, Monocytes), absolute cell number of Neutrophil + Gr-1 + , CCR3 mRNA expressions(by Real-time PCR), IL-4, IFN-Ɣ production levels(by ELISA), histologic test(by H&E staining, toluidine blue staining). Results : The results of making NC/Nga mouse induced atopic dermatitis swim in tanks filled with DOGO phreatic water(contain sulphur) are as follows.

Inhibitory effects of Taraxacum mongolicum with phreatic water on melanin synthesis

“…65 Another study used PW from Dogo Hot Springs and found that it effectively controls allergic inflammatory response and that it can be used for atopic skin disorder by using atopic dermatitis induction NC/Nga mouse. 22 …”

“…Accordingly, this study was intended to figure out if using TAM together with PW from Dogo Hot Springs increases the inhibitory effect on melanin generation, as previous studies proved that TAM inhibits melanin generation and another previous study on organs analyzed the components of PW from Dogo Hot Springs. 22 …”

“…The natural PW was collected from Paradise Spa Dogo, Korea. Component analysis on the PW of Dogo Hot Springs had been conducted in the study of Jang et al 22 …”

“…Although the whitening effect of phreatic water (PW) 20 , 21 —which is widely used for the treatment of skin disorders such as psoriasis, atopic dermatitis, pimples, and xeroderma—has not been studied yet, this study tried to verify if PW, together with TAM, enhances the inhibitory effect on melanin generation. For PW, we used the water from Dogo Hot Springs, whose sulfuric content is known to effectively control allergic inflammatory responses (see the recent study of Jang et al 22 ). UVB was irradiated on HRM-2 melanin-possessing hairless mice.…”

Melanin Synthesis Inhibitory Effect of Eriobotryae Folium Extracts & Eriobotryae Folium and Phreatic Water Mixture

“…Conclusions: EF and PW might be a new and effective treatment for whitening and treating pigmentation of skin.The water was collected from Paradise Spa Dogo, Korea. The component analysis of the PW of DOGO had been conducted in the research of researcher Jang [8], and drinking water test was investigated by Chungnam Institute of Health and Environment, Korea.…”