Effect of Dopamine on Viability of BHK-21 Cells

Abstract

We studied the effects of dopamine added to culture medium on survival of floating or adherent BHK-21 cells differing by organization of actin cytoskeleton. The viability of floating cells more drastically decreased with increasing dopamine concentration and duration of exposure than that of adherent cells. The cells worse adhered to the substrate and formed a monolayer. The formed monolayer degrades, cell borders become blurred, cells, polygonal in the control, are rounded. Preliminary blockade of dopamine receptors with haloperidol, inessential for cell survival and morphology, does not prevent the destructive effect of dopamine on the cells. Ultrastructural study revealed increased density of filamentous actin threads in deep compartments of cell cytoplasm after dopamine treatment, this increase being more pronounced in cells grown in suspension. Bearing in mind the polymerizing effect of dopamine on globular actin in vitro and the fact that the content of this protein in floating cells is higher than in adherent cells, we can conclude that the decrease in viability of BHK-21 cells is caused by interaction of dopamine with cytoplasmic globular actin.

First Experimental Evidence of Dopamine Interactions with Negatively Charged Model Biomembranes

“…28 The mechanism of antioxidant effects of DA has not yet been examined in regard to DA interactions with lipid membranes. It is known that DA alters the cell ultrastructure and organization of actin cytoskeleton, 29,30 but, to our surprise, the only publication on DA/biomembrane interactions we have found was a molecular dynamics study assisted by a few measurements of surface pressure of lipid monolayer containing DA, a paper published very recently by Orłowski et al 31 Therefore, we decided to carry out a series of experiments in order to obtain thermodynamic description of DA/membrane interactions. According to our knowledge, this is the first experimental study in this field.…”

Antimicrobial Activities of Zn-Doped CuO Microparticles Decorated on Polydopamine against Sensitive and Antibiotic-Resistant Bacteria

“…47 Dopamine also alters the cell ultrastructure and organization of the actin cytoskeleton of mammalian cells. 48,49 The precise PDA structure is not established; however, it is often presented as a catechol/ amine/quinone/indole heteropolymer or eumelanine-like oligoindoles. 47 This study confirmed that PDA-Zn@CuO completely inhibited the growth of E. coli as confirmed by an SEM micrograph obtained for this affected bacterium (Figure 5).…”