Effect of double-muscling in Belgian Blue young bulls on the intramuscular fatty acid composition with emphasis on conjugated linoleic acid and polyunsaturated fatty acids

In a 2 × 2 factorial design, 12 Thai Native and 12 Holstein bulls were fed ad libitum a total mixed ration (20 : 80; roughage : concentrate) with whole cottonseed (WCS) or sunflower seed (SFS) as oilseed sources. The rations contained 7% crude fat and were fed for 90 days. Plasma was taken at three times during the experiment, and at slaughter the longissimus dorsi and subcutaneous fat were sampled for fatty acid analysis. Ration did not affect rumen fermentation parameters. The plasma fatty acid profile was not affected by ration. In subcutaneous fat, a ration × breed interaction for the saturated fatty acid (SFA) and c9t11 CLA proportions was observed, resulting from larger differences between the rations in Thai Native compared with Holstein bulls. The WCS ration resulted in higher proportions of SFA and lower proportions of monounsaturated fatty acids and c9,t11 CLA compared with the SFS ration ( P < 0.01). In the intramuscular fat, the WCS ration was also associated with a lower c9t11 CLA proportion ( P < 0.01) and higher SFA proportion ( P < 0.05). The intramuscular proportion of polyunsaturated acids was higher and the proportion of SFA was lower in Thai Native compared with Holstein bulls ( P < 0.05), irrespective of ration.

Section: Methodsmentioning

confidence: 99%

Effect of whole cottonseed v. sunflower seed on the fatty acid profile of subcutaneous fat, longissimus dorsi and blood of Thai Native and Holstein bulls

Polviset

Schonewille

Everts

et al. 2015

“…0.006 6 0.001 0.006 6 0.001 0.06 6 0.01 C20:3n-6 0.004 6 0.001 0.012 6 0.001 0.04 6 0.01 C20:4n-6 0.012 6 0.003 0.013 6 0.003 0.31 6 0.01 C22:4n-6 0.029 6 0.004 0.019 6 0.003 0.14 6 0.02 C18:3n-3 2.96 6 0.15 21.62 6 0.79 2.90 6 0.25 C18:4n-3 0.021 6 0.004 0.031 6 0.009 0.71 6 0.01 C20:5n-3 0.11 6 0.03 0.16 6 0.06 4.91 6 0.12 C22:5n-3 0.020 6 0.001 0.020 6 0.007 0.60 6 0.03 C22:6n-3 0.10 6 0.02 0.12 6 0.04 3.42 6 0.03 (Raes et al, 2001). The gas chromatography conditions were: injector: 2508C, detector: 2808C and H 2 as carrier gas.…”

Section: Animals and Dietsmentioning

confidence: 99%

Effect of supplementation of the maternal diet with fish oil or linseed oil on fatty-acid composition and expression of Δ5- and Δ6-desaturase in tissues of female piglets

Missotten

Smet

Raes

et al. 2009

Self Cite

The present study investigated whether enrichment of the pig maternal diet with n-3 polyunsaturated fatty acids (PUFA) affects the fatty-acid composition of female piglets via enhancing of expression of the lipogenic enzymes D5-desaturase (D5d) and D6-desaturase (D6d). The sows (50% Landrace 3 50% Large White) were fed a control diet or one of the experimental diets starting at day 45 in gestation. The experimental diets were supplemented either with linseed oil or fish oil, whereas the control diet contained palm oil. Expression of D5d and D6d, and fatty-acid composition was determined by Western blotting and gas-liquid chromatography, respectively, in muscle, subcutaneous adipose tissue and liver. The highest D5d protein expression was observed in the piglets' muscle, followed by subcutaneous adipose tissue, with the lowest level in the liver. Expression of D6d in the piglets' tissues followed an opposite pattern, and was highest in the liver, followed by subcutaneous adipose tissue, with the lowest level in muscle. Supplementation of the maternal diet with fish oil or linseed oil increased the level of n-3 PUFA of the piglets in a tissue-specific manner. The response of D6d and D5d protein expression in female piglets, with average birth weight 2.4 kg, to the dietary manipulation was also tissue-specific. It is suggested that the increase in n-3 PUFA content in the progeny was related, at least partially, to the activation of D6d and D5d expression.

“…Meat samples were extracted in duplicate as described by Raes et al (2001). Briefly, 5 g of meat was homogenised for 30 s at 9000 r.p.m.…”

Section: Pasturesmentioning

confidence: 99%

“…Subcutaneous fat samples (1 g) were extracted using a similar procedure as described before for FA extraction of meat (Raes et al, 2001), however the bottom layer was recovered into volumetric flasks after washing with distilled water and was brought to a final volume of 100 ml with C/ M (2/1, vol/vol). Pasture composition affects lamb fatty acid metabolism Methylation.…”

Section: Pasturesmentioning

confidence: 99%

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Effect of grazing pastures with different botanical composition by lambs on rumen fatty acid metabolism and fatty acid pattern of longissimus muscle and subcutaneous fat

Lourenço

Ranst

Smet

et al. 2007

Self Cite

In order to study the effect of grazing pastures with a different botanical composition on rumen and intramuscular fatty acid metabolism, 21 male lambs were assigned to three botanically different pastures: botanically diverse (BD) (consisting for 65% of a variety of grass species); Leguminosa rich (L) (consisting for 61% of Leguminosae) and intensive English ryegrass (IR) (with 69% Lolium perenne). Pastures were sampled weekly for 12 weeks for analysis of their fatty acid content and composition and on nine occasions to determine the botanical composition. Ruminal and abomasal contents were sampled at slaughter and muscle and subcutaneous fat 24 h after slaughter. All samples were prepared and analysed for fatty acid composition. The L pasture showed a higher fatty acid content (29.8 mg/g dry matter (DM) v. 18.5 and 25.5 mg/g DM, for BD and IR pastures, respectively), but the sum of the proportions of the major polyunsaturated fatty acids, C18:2 n-6 and C18:3 n-3, were similar for the three pastures (69.9, 69.4 and 71.1% of fatty acids methyl esters (FAME) for BD, L and IR pastures, respectively). The BD pasture was richer in C18:2 n-6 (18.2% of FAME), while IR pasture had a higher C18:3 n-3 content (57.2% of FAME). Rumen data showed that animals grazing the BD pasture presented higher proportions of biohydrogenation intermediates, mainly C18:1 t11, C18:2 t11c15 and CLA c9t11, suggesting an inhibition of biohydrogenation. These changes were associated with shifts in the rumen microbial population as indicated by differences in the rumen pattern of volatile fatty acids, microbial odd-and branched-chain fatty acids. In L pasture animals, the content of C18:2 n-6 and C18:3 n-3 in the abomasum and subcutaneous fat was higher. Finally, higher proportions of C20:4 n-6, C20:5 n-3 and C22:5 n-3 and higher indices for elongation and desaturation activity in the intramuscular fat of BD grazing animals suggest some stimulation of elongation and desaturation of long-chain fatty acids, although this also might have been provoked partially by reduced fat deposition (due to a lower growth rate of the animals).