Effect of duration of incubation of<i>Mycoplasma gallisepticum</i>cultures on the sensitivity and specificity of antigens for ELISA and microimmunofluorescence tests

Loughnane, J. Paul; Bradbury, Janet M.; Jordan, F. T. W.

doi:10.1080/03079459308418935

Cited by 3 publications

(1 citation statement)

References 26 publications

(27 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Against MG. Ten chickens from both group(1), group (3) and group (4) were challenged 4weeks post 1 st vaccination using 0.1ml of an overnight virulent R. strain of M. gallisepticum containing (3.8 × 10 6 CFU/ml) according to Longhnane et al, (1993). Second challenge was done 4 weeks post boostering in another 10 chickens from the same groups.…”

Section: Methodsmentioning

confidence: 99%

Trial for preparation and evaluation of combined vaccine against ND, IB and M. gallisepticum diseases in chickens

Salama

Hasan

Mohammed

et al. 2010

Journal of Veterinary Medical Research

View full text Add to dashboard Cite

In this study, a combined Trivalent vaccine against ND, IB and M. gallisepticum was locally prepared and evaluated in comparison with other locally prepared Bivalent ND and IB and monovalent M. gallisepticum vaccines. The obtained results were promising for this locally prepared Trivalent vaccine and the immune response was outstanding starting at the 2 nd week post vaccination and showed extended raising allover the experiment period. The immune response of chickens vaccinated with the Trivalent was shoot up post boostering at the 8 th week post 1 st vaccination. These results were confirmed and supported by the challenge tests using the virulent strains of the three pathogens. So it could be recommend that the production of this Trivalent ND, IB and M. gallisepticum will help in the control of the three diseases and their complications.

show abstract

Section: Methodsmentioning

confidence: 99%

Trial for preparation and evaluation of combined vaccine against ND, IB and M. gallisepticum diseases in chickens

Salama

Hasan

Mohammed

et al. 2010

Journal of Veterinary Medical Research

View full text Add to dashboard Cite

show abstract

Haemagglutinins of pathogenic avian mycoplasmas

Benčina

2002

Avian Pathology

View full text Add to dashboard Cite

The pathogenic avian mycoplasmas, Mycoplasma gallisepticum, Mycoplasma synoviae, Mycoplasma meleagridis, Mycoplasma iowae and Mycoplasma imitans, synthesize haemagglutinins that are immunogenic, variably expressed, surface proteins. The haemagglutinins of M. gallisepticum (pMGA), M. synoviae (VlhA) and M. imitans are lipoproteins, encoded by related multigene families that appear to have arisen by horizontal gene transfer. M. gallisepticum also has genes encoding cytadhesins in its genome but these are present as a single copies, while the pMGA gene family contains 30 to 70 genes. The switch in expression of distinct pMGA genes (e.g. pMGA1.1 to pMGA1.9) generates antigenic variation, which is thought to be important in immune evasion but also has significance in the preparation of M. gallisepticum antigens for serological diagnosis. In the majority of M. synoviae strains, post-translational cleavage of the VlhA protein generates an amino-terminal part (the lipoprotein MSPB) and a carboxyl-terminal part (MSPA), which mediates binding to erythrocytes. The 5'vlhA gene region, which encodes proline-rich repeats in the amino-terminal part of MSPB, is highly polymorphic among M. synoviae strains. Insertions or deletions in the part of vlhA encoding the proline-rich repeats cause MSPB length variation in different M. synoviae strains. Recombination between the 5'vlhA gene and pseudogenes in the genome generates changes in antigenic determinants in the carboxyl two-thirds of the MSPB molecule, and in MSPA, resulting in changes in the domains involved in the binding of M. synoviae to erythrocytes. Variant haemagglutinins of M. gallisepticum (pMGA1.7) and M. synoviae (diverse VlhA forms) share sequences that may be responsible for antigenic cross-reactions between M. gallisepticum and M. synoviae. Shared epitopes have been demonstrated using specific antibodies against MSPB that also recognize proteins of M. gallisepticum and of M. iowae (serotype N). Size and antigenic variants have also been reported for M. meleagridis and M. iowae proteins, but it is not known if these are their haemagglutinins. Advances in the molecular characterization of M. gallisepticum (pMGA, pvpA) and M. synoviae (vlhA) genes and their sequencing in numerous strains is likely to enable significantly improved epidemiological studies and improved tracing of M. gallisepticum and M. synoviae strains in different flocks.

show abstract

Application of Chicken Immune Sera to Identification of Mycoplasmas by the Filter Immunobinding Method.

1998

View full text Add to dashboard Cite

Application of chicken immune sera to identification of Mycoplasma species by the filter immunobinding (FIB) method was investigated. Immune sera against type strains of 15 Mycoplasma species were prepared in chickens by inoculation with concentrated Mycoplasma organisms. Chicken immune sera, when absorbed in a broth medium and diluted in a blocking solution, showed a definite specificity with homologous Mycoplasma antigens by FIB. The identification results of field isolates of Mycoplasma gallisepticum and M. synoviae by FIB using absorbed chicken immune sera were consistent with those obtained by conventional tests based on biochemical properties and growth inhibition using rabbit immune sera. Chicken immune sera were evidenced to be useful for identification of Mycoplasma species when applied to FIB although they were not applicable in growth and metabolism inhibition tests.

show abstract

Effect of duration of incubation ofMycoplasma gallisepticumcultures on the sensitivity and specificity of antigens for ELISA and microimmunofluorescence tests

Cited by 3 publications

References 26 publications

Trial for preparation and evaluation of combined vaccine against ND, IB and M. gallisepticum diseases in chickens

Trial for preparation and evaluation of combined vaccine against ND, IB and M. gallisepticum diseases in chickens

Haemagglutinins of pathogenic avian mycoplasmas

Application of Chicken Immune Sera to Identification of Mycoplasmas by the Filter Immunobinding Method.

Contact Info

Product

Resources

About