Effect of Early Introduction of Microencapsulated Diet to Larval Atlantic Halibut, Hippoglossus hippoglossus L. Assessed by Microarray Analysis

Murray, Harry M.; Lall, Santosh P.; Rajaselvam, Rajesh; Boutilier, Lee Anne; Flight, Robert M.; Blanchard, Brian; Colombo, Stefanie M.; Mohindra, Vindhya; Yúfera, Manuel; Douglas, Susan E.

doi:10.1007/s10126-009-9211-4

Cited by 22 publications

(29 citation statements)

References 65 publications

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“…Atlantic halibut larvae using a custom microarray (Douglas et al 2008;Murray et al 2010). The limited number of sequences derived from early embryonic tissues in the microarray platforms used in these studies underscores the importance of having large genome resources to advance our understanding of the molecular mechanisms driving developmental processes in fish larvae.…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic Characterization of the Larval Stage in Gilthead Seabream (Sparus aurata) by 454 Pyrosequencing

et al. 2011

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Gilthead seabream (Sparus aurata) is a teleost belonging to the family Sparidae with a high economical relevance in the Mediterranean countries. Although genomic tools have been developed in this species in order to investigate its physiology at the molecular level and consequently its culture, genomic information on post-embryonic development is still scarce. In this study we have investigated the transcriptome of a marine teleost during the larval stage (from hatching to 60 days after hatching) by the use of 454-pyrosequencing technology. We obtained a total of 68,289 assembled contigs, representing putative transcripts, belonging to 54,606 different clusters.Comparison against all S. aurata expressed sequence tags (ESTs) from the NCBI database revealed that up to 34,722 contigs, belonging to about 61 % of gene clusters, are sequences previously not described. Contigs were annotated through an iterative Blast pipeline by comparison against databases such as NCBI RefSeq from Danio rerio, Swissprot or NCBI teleost ESTs. Our results indicate that we have enriched the number of annotated sequences for this species by more than 50 % compared with previously existing databases for the gilthead seabream. Gene Ontology analysis of these novel sequences revealed that there is a statistically significant number of transcripts with key roles in larval development, differentiation, morphology and growth. Finally, all information has been made available online through user-friendly interfaces such as GBrowse and a Blast server with a graphical frontend.

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Section: Discussionmentioning

confidence: 99%

Transcriptomic Characterization of the Larval Stage in Gilthead Seabream (Sparus aurata) by 454 Pyrosequencing

et al. 2011

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“…replacement of fish meal protein by soy proteins), or whether the expression of genes involved in digestion and absorption of macronutrients are altered in the larvae as a result of the introduction of a microencapsulated diet (Murray et al 2009(Murray et al , 2010. The first study showed that Atlantic halibut juveniles tolerate up to 30 % soy protein in the diet, although microarray analysis showed that the expression of several immune markers and genes involved in detoxification increased (Murray et al 2009).…”

Section: Changes In Transcriptome During Larval Development and Nutrimentioning

confidence: 99%

“…The first study showed that Atlantic halibut juveniles tolerate up to 30 % soy protein in the diet, although microarray analysis showed that the expression of several immune markers and genes involved in detoxification increased (Murray et al 2009). Microdiet particles can be readily consumed by halibut larvae after a period of adaptation, providing an adequate source of nutrients with no significant increase in mortality (Murray et al 2010). However, growth of the larvae seems to be impaired and accompanied by an increased incidence of malpigmentation of the eye and the skin, which correlates with changes in the expression of genes involved in pigmentation and eye development (Murray et al 2010).…”

Section: Changes In Transcriptome During Larval Development and Nutrimentioning

confidence: 99%

“…Microdiet particles can be readily consumed by halibut larvae after a period of adaptation, providing an adequate source of nutrients with no significant increase in mortality (Murray et al 2010). However, growth of the larvae seems to be impaired and accompanied by an increased incidence of malpigmentation of the eye and the skin, which correlates with changes in the expression of genes involved in pigmentation and eye development (Murray et al 2010). These studies thus suggest that microarray analysis can be a suitable approach to optimize nutritional manipulations in flatfishes and to establish associated physiological alterations.…”

Section: Changes In Transcriptome During Larval Development and Nutrimentioning

confidence: 99%

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Advances in genomics for flatfish aquaculture

Cerdà

Manchado

2012

Genes Nutr

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Fish aquaculture is considered to be one of the most sustainable sources of protein for humans. Many different species are cultured worldwide, but among them, marine flatfishes comprise a group of teleosts of high commercial interest because of their highly prized white flesh. However, the aquaculture of these fishes is seriously hampered by the scarce knowledge on their biology. In recent years, various experimental 'omics' approaches have been applied to farmed flatfishes to increment the genomic resources available. These tools are beginning to identify genetic markers associated with traits of commercial interest, and to unravel the molecular basis of different physiological processes. This article summarizes recent advances in flatfish genomics research in Europe. We focus on the new generation sequencing technologies, which can produce a massive amount of DNA sequencing data, and discuss their potentials and applications for de novo genome sequencing and transcriptome analysis. The relevance of these methods in nutrigenomics and foodomics approaches for the production of healthy animals, as well as high quality and safety products for the consumer, is also briefly discussed.

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“…After this time, MS-222 was added to the tanks (Murray et al 2010) and tanks were covered with an opaque board to cease the trial. To adjust light intensity without altering the light spectrum, we used wooden frames that completely covered the top of the experimental tanks and stretched white linen sheets over the frames (Utne-Palm & Stiansen 2002).…”

Section: Light Intensitymentioning

confidence: 99%

Effect of light intensity, prey density, and ontogeny on foraging success and prey selection of larval yellow perch (Perca flavescens)

Martin

Wahl

Czesny

2012

Ecology of Freshwater Fish

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Light intensity has been shown to influence the foraging success of larval fish. However, the effect of light intensity on larval foraging is likely variable and influenced by both the density and characteristics of planktonic prey. In this study we examined the influence of light intensity of 0.1, 2.0, and 60 lmol·s À1 ·m À2 Photosynthetically Active Radiation (PAR) on foraging of yellow perch (Perca flavescens) larvae at two prey densities. We fed them with a mixture of zooplankton taxa common to lakes inhabited by yellow perch. In addition to light intensity and prey density, the effect of larval yellow perch size was examined by using fish ranging from 9 to 15 mm. The results of our study indicated that yellow perch larvae are well adapted to feed at a wide range of light intensities, as there was no difference in foraging success at investigated light intensities. Increasing prey density from 25 to 150 (zooplankton·l À1 ) significantly improved the foraging success of larval yellow perch. However, the influence of prey density on foraging success was dependent on fish length. Improved foraging success at increased prey densities occurred only for individuals with a total length >10 mm. Overall, prey selection by fish larvae was influenced by light intensity, prey density, and fish length. However, the factors that influenced selection for specific prey types differed. Our study, combined with evidence from other field and laboratory work, highlight the need for a better understanding of the influence of prey density on foraging throughout ontogeny.

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Effect of Early Introduction of Microencapsulated Diet to Larval Atlantic Halibut, Hippoglossus hippoglossus L. Assessed by Microarray Analysis

Cited by 22 publications

References 65 publications

Transcriptomic Characterization of the Larval Stage in Gilthead Seabream (Sparus aurata) by 454 Pyrosequencing

Transcriptomic Characterization of the Larval Stage in Gilthead Seabream (Sparus aurata) by 454 Pyrosequencing

Advances in genomics for flatfish aquaculture

Effect of light intensity, prey density, and ontogeny on foraging success and prey selection of larval yellow perch (Perca flavescens)

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