Effect of ecdysone agonists on vitellogenesis and the expression of EcR and USP in codling moth (<i>Cydia pomonella</i>)

Sun, Xiaoping; Song, Qisheng; Barrett, Bruce A.

doi:10.1002/arch.10073

Cited by 28 publications

(20 citation statements)

References 43 publications

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“…Although ecdysteroids were not measured in this study, it is reasonable to hypothesize that the effects that we observed on the fecundity of S. exigua also could be due to changes in ecdysteroid concentrations caused by methoxyfenozide in the eggs of this insect. If so, then vitellogenesis and embryogenesis were altered, as has previously been reported in C. pomonella (Sun et al 2003). This hypothesis is supported by a study carried out by Berghiche et al (2008) in which halofenozide was observed to increase the amounts of both free ecdysteroids and 20E in T. molitor eggs.…”

Section: Discussionsupporting

confidence: 64%

“…This effect could explain the reduction in reproductive parameters reported previously in studies using ecdysone agonists in several insect pests (Farinó s et al 1999;Knight 2000;Sun et al 2000Sun et al , 2003Taibi et al 2003;Saenz-de-Cabezon et al 2005;Pineda et al 2006;Osorio et al 2008,). Future studies should clarify where and how methoxyfenozide acts to cause the observed changes in the concentrations of carbohydrates, proteins, and lipids.…”

Section: Discussionmentioning

confidence: 70%

“…Other studies have reported that the levels of vitellogenins, which are the precursors of insect egg yolk proteins, in the hemolymph of C. pomonella and L. decemlineata females were signiÞcantly increased when these species were exposed to tebufenozide, methoxyfenozide, or halofenozide, either through contact with treated surfaces or by topical application (Sun et al 2003, Farinó s et al 1999. These Þndings suggest that oocyte degeneration was followed by reabsorption of these proteins from the ovaries and that, consequently, an inhibition of oviposition occurred (Farinó s et al 1999).…”

Section: Discussionmentioning

confidence: 78%

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Long-Term Effects of Methoxyfenozide on the Adult Reproductive Processes and Longevity of Spodoptera exigua (Lepidoptera: Noctuidae)

Luna¹,

Robinson²,

Martínez³

et al. 2011

jnl. econ. entom.

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The long-term effects of methoxyfenozide on the longevity and reproductive processes of beet armyworm, Spodoptera exigua (Hübner) (Lepidoptera: Noctuidae), adults were assessed after exposure by ingestion. Methoxyfenozide significantly reduced adult male longevity compared with females by 1.1 and 1.5 d at 75 and 150 mg (AI)/liter, respectively. Fecundity decreased by >60% with both concentrations at 72 and 96 h after treatment, but at 48 h, no significant effect was observed. The carbohydrate, protein, and lipid content in the eggs were determined as representatives of the biochemical effects of methoxyfenozide associated with the disruption of reproductive processes. The content of carbohydrates in the eggs laid 48 h at treatment was similar to that of controls, but it increased by approximately 1.5 and 2-fold in eggs laid after 72 and 96 h, respectively, compared with controls (15 microg per egg). Protein content was reduced approximately 2.5 and approximately 3-fold for each treatment concentration, respectively, compared with the controls (25 and 23 microg per egg for 75 and 150 mg [AI]/liter, respectively) in eggs collected 72 and 96 h after treatment. Lipid content significantly decreased by approximately 1.6-fold in both treatment concentrations in eggs collected at 48 and 96 h after treatment compared with the controls (24 and 21 microg per egg for 48 and 96, respectively), but it was similar to controls (approximately 19 microg per egg) at 72 h (approximately 15 microg per egg) for both concentrations. The biochemical effects of methoxyfenozide on S. exigua egg formation detected in this work are consistent with the reduction in fertility observed, as reported previously.

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Section: Discussionsupporting

confidence: 64%

Section: Discussionmentioning

confidence: 70%

Section: Discussionmentioning

confidence: 78%

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Long-Term Effects of Methoxyfenozide on the Adult Reproductive Processes and Longevity of Spodoptera exigua (Lepidoptera: Noctuidae)

Luna¹,

Robinson²,

Martínez³

et al. 2011

jnl. econ. entom.

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“…Seth et al (2004) have reported the deposition of RH-5992 in the gonads of both male and female S. littoralis leading to sterility. Reduction in fecundity and fertility in codling moth Cydia pomonella (L., 1758) was also reported by Sun et al (2000Sun et al ( , 2003.…”

Section: Discussionsupporting

confidence: 54%

Effect of RH-2485 on development, metamorphosis and synthesis of major proteins in female silkworm Bombyx mori

2010

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Toxicological data on silkworm Bombyx mori are quite comparable to those of other lepidopteran pest insects, therefore, it is considered as a suitable model for exploring effects of any new synthetic formulations. In this study, female V instar larvae of silk moth B. mori were chosen to evaluate the lethal and sublethal toxicity effects of RH-2485 (methoxyfenozide), a non-steroidal ecdysteroid agonist and to substantiate the ecdysteroid mimicking action of RH-2485 on ovary development, vitellogenin incorporation and egg production in isolated pupal abdomen (IPA). Probit analysis was carried out to find the median lethal dose (LD50) from 96 h cumulative mortality percent. Protein profile of haemolymph, fat body, ovary and eggs were separated in SDS-PAGE. Western blot analysis was carried out to confirm vitellogenin in the ovary. Sublethal effects on feeding, cocoon spinning, pupation, adult emergence and egg production were studied at doses of 1/5 th , 1/10 th and 1/20 th of LD50. Significant changes were observed in all these parameters at all three sublethal doses. The morphological effects were related to underlying biochemical changes by finding the changes in haemolymph, fat body, ovary and egg protein profile. Marked changes were observed in storage proteins (80 kDa) and 30 kDa proteins in the haemolymph at all three sublethal doses. The larvae that escaped the sublethal effects at a dose of 1/20 of LD50 and emerged as adults with malformed wings produced significantly lower number of eggs. The isolated pupal abdomen (IPA) treated with RH-2485 did not metamorphose into adult but the oocyte development and vitellogenesis were normal but the egg precursor processing was incomplete leading to failure in choriogenesis.

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“…An earlier survey of the toxic effects of RH 5849 on different orders of insects indicated that one species of dipterans (Neobellieria bullata) was affected deleteriously (Darvas et al 1992). In addition to disrupting development of insect larvae, the compounds also affected diapause and reproduction of treated insects (Gadenne et al, 1990;Swevers and Iatrou, 1999;Sun et al, 2003;Seth et al, 2004). Moreover, treatment with the compound RH 5849 triggered yolk polypeptide synthesis (which is normally characteristic of females) in several species of male flies including Drosophila and Phormia (DeLoof et al, 1997).…”

Section: Archives Of Insect Biochemistry and Physiologymentioning

confidence: 95%

Comparative larvicidal toxicities of three ecdysone agonists on the mosquitoes Aedes aegypti, Culex quinquefasciatus, and Anopheles gambiae

Beckage

Marion

Walton

et al. 2004

Arch Insect Biochem Physiol

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Ecdysone agonists are hormonally active insect growth regulators that disrupt development of pest insects and have potential for development as insecticides. Their effects have been particularly well-studied in Lepidoptera and Coleoptera, but significantly less is known about their effects on dipterans, particularly aquatic species. The potency of three ecdysone agonists on larvae of 3 mosquito species, Aedes aegypti, Anopheles gambiae, and Culex quinquefasciatus, was examined. Anopheles gambiae was the most susceptible species and Ae. aegypti was the most resistant species to the effects of the three compounds tested. Potency, in descending order, was RH-2485 > RH-5992 > RH-5849. Dose-response relationships were determined for the three agonists; RH-2485 was found to be the most effective endocrine disruptor against all three species. The observed biological effects of these compounds were similar to those reported for other insects, and mosquitoes initiated molting and apolysis but did not complete a molt. In some cases, mosquito larvae synthesized a new cuticle that appeared to be normally sclerotized but the larvae failed to ecdyse and shed the exuvium. These compounds may prove to be valuable insect growth regulators for control of mosquitoes to decrease the frequency of pathogen transmission to humans. Prospects for using these compounds to control mosquitoes in the field are discussed, along with possible impacts on non-target arthropods in mosquito habitats.

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Effect of ecdysone agonists on vitellogenesis and the expression of EcR and USP in codling moth (Cydia pomonella)

Cited by 28 publications

References 43 publications

Long-Term Effects of Methoxyfenozide on the Adult Reproductive Processes and Longevity of Spodoptera exigua (Lepidoptera: Noctuidae)

Long-Term Effects of Methoxyfenozide on the Adult Reproductive Processes and Longevity of Spodoptera exigua (Lepidoptera: Noctuidae)

Effect of RH-2485 on development, metamorphosis and synthesis of major proteins in female silkworm Bombyx mori

Comparative larvicidal toxicities of three ecdysone agonists on the mosquitoes Aedes aegypti, Culex quinquefasciatus, and Anopheles gambiae

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