Effect of EDTA on the resistance of clinical isolates of Acinetobacter baumannii to the bactericidal activity of normal human serum

García, Antonio Manuel Rodríguez; Solar, Henry; González, Carlos; Zemelman, R

doi:10.1099/0022-1317-49-11-1047

Cited by 20 publications

(20 citation statements)

References 8 publications

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“…Time between donation and experimental testing could facilitate platelet degradation. Additionally, the non-specific activity of the complement system and antigen-specific antibodies present in donor plasma could play an operative role [36,37]. Complement acts against non-self invaders by detecting pathogen-associated molecular patterns present on the surface of most bacteria [36].…”

Section: Resultsmentioning

confidence: 99%

Human platelet gel supernatant inactivates opportunistic wound pathogens on skin

et al. 2014

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Activation of human platelets produces a gel-like substance referred to as platelet rich plasma or platelet gel. Platelet gel is used clinically to promote wound healing; it also exhibits antimicrobial properties that may aid in the healing of infected wounds. The purpose of this study was to quantify the efficacy of human platelet gel against the opportunistic bacterial wound pathogens Acinetobacter baumannii, Pseudomonas aeruginosa, and Staphylococcus aureus on skin. These opportunistic pathogens may exhibit extensive antibiotic resistance, necessitating the development of alternative treatment options. The antimicrobial efficacy of platelet gel supernatants was quantified using an in vitro broth dilution assay, an ex vivo inoculated skin assay, and in an in vivo skin decontamination assay. Human platelet gel supernatants were highly bactericidal against A. baumannii and moderately but significantly bactericidal against S. aureus in vitro and in the ex vivo skin model. P. aeruginosa was not inactivated in vitro; a low but significant inactivation level was observed ex vivo. These supernatants were quite effective at inactivating a model organism on skin in vivo. These results suggest application of platelet gel has potential clinical applicability, not only in the acceleration of wound healing, but also against relevant bacteria causing wound infections.

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Section: Resultsmentioning

confidence: 99%

Human platelet gel supernatant inactivates opportunistic wound pathogens on skin

et al. 2014

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“…(Hellwage et al, 2001;Hartmann et al, 2006). García et al (2000) reported that the serum resistance of A. baumannii was mainly due to lipopolysaccharides. These complement regulator-acquiring surface proteins contribute to persistence of bacteria in the hosts and to pathogenesis of Lyme disease.…”

Section: Discussionmentioning

confidence: 99%

Serum resistance ofAcinetobacter baumanniithrough the binding of factor H to outer membrane proteins

Kim

Choi

Moon

et al. 2009

FEMS Microbiology Letters

139

109

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Bacteremia is a common systemic disease caused by Acinetobacter baumannii, an important hospital-acquired pathogen among critically ill patients. The complement system is central to innate immune defense against invading bacteria in the blood. The present study investigated the susceptibility of clinical A. baumannii isolates to normal human sera (NHS), and determined the resistance mechanism of A. baumannii against complement-mediated lysis. The survival of A. baumannii isolates from bacteremic patients was significantly decreased in undiluted NHS, but they were resistant to 40% NHS. The alternative complement pathway was responsible for the direct killing of bacteria. The main regulator of the alternative complement pathway, factor H, bound to the surface of live A. baumannii treated with NHS. Factor H interacted with the outer membrane proteins with molecular sizes of 38 (AbOmpA), 32, and 24 kDa. The isogenic AbOmpA(-) mutant was highly susceptible to NHS in comparison with the wild-type A. baumannii strain, suggesting that AbOmpA was an important complement regulator-acquiring surface protein. These results indicate that A. baumannii evades complement attack through the acquisition of factor H to their surface.

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“…The complement system is the host innate immune defense comprising a series of serum proteins that initiates the death of the bacterium through either lysis or opsonization. One of the mechanisms by which bacterial cells resist killing by serum compliments is by producing surface proteins that bind human factor H (FH), and thereby inhibit the deposition of complements on the bacterial surface (41,63). The mechanism by which Acinetobacter spp.…”

Section: Pathogenesis Virulence Factors and Resistancementioning

confidence: 99%

The Ecology, Biology and Pathogenesis of Acinetobacter spp.: An Overview

et al. 2011

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Acinetobacter are a major concern because of their rapid development of resistance to a wide range of antimicrobials, and rapid profundity in transformation, surviving desiccation and persistinge in the environment for a very long time. The organisms are associated with bacteraemia, pulmonary infections, meningitis, diarrhea and notorious nosocomial infections with mortality rates of 20 to 60%. Transmission is via person-to-person contact, water and food contamination, and contaminated hospital equipment. The increasing virulence and rapid development of multidrug resistance by these organisms highlights the need to search for alternatives for chemotherapy. A poor understanding of the organisms and dearth of information about their occurrence especially in developing countries informed the need for this review paper.

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Effect of EDTA on the resistance of clinical isolates of Acinetobacter baumannii to the bactericidal activity of normal human serum

Cited by 20 publications

References 8 publications

Human platelet gel supernatant inactivates opportunistic wound pathogens on skin

Human platelet gel supernatant inactivates opportunistic wound pathogens on skin

Serum resistance ofAcinetobacter baumanniithrough the binding of factor H to outer membrane proteins

The Ecology, Biology and Pathogenesis of Acinetobacter spp.: An Overview

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