Effect of Eight Weeks Aerobic and Combined Training on Serum Levels of Sirtuin 1 and PGC-1α in Coronary Artery Bypass Graft Patients

Abstract

Background and objectives: Sirtuin-1 (SIRT1) is a conserved a NAD +-dependent protein deacetylase essential for cellular metabolism and defence against oxidative stress and endothelial dysfunction. This study aimed to compare effects of 8 weeks of aerobic and combined training on serum level of SIRT1 and PGC1-α in coronary artery bypass graft patients. Methods: In this semi-experimental study, 45 non-active men (mean age 47.4 ± 3.3 years) who had undergone coronary artery bypass graft surgery were randomly divided into three groups: aerobic training, combined resistance and aerobic training and control. Trainings were performed at 80% of maximum heart rate, three sessions a week for 8 weeks. Blood samples were collected 24 hours before the first exercise session and 48 hours after the last training session. T-test and one-way analysis of variance were used for assessment of within-group and between-group differences, respectively. P-values less than 0.05 were considered as statistically significant. Results: The 8-week aerobic and combined training increased serum levels of SIRT1 and PGC-1α compared with the control group, and significantly decreased the weight of subjects (p=0.001). There was no significant difference in serum levels of SIRT1 and PGC-1α between the two training groups (P≥0.05). Conclusion: Both combined and aerobic training significantly improve the SIRT1 and PGC-1α levels in coronary artery bypass graft patients.

Mitochondrial Dysfunction Contributes to Aging‐Related Atrial Fibrillation

“…After incubation at room temperature for 10 minutes, the reaction was terminated by the addition of acid, and absorbance was measured at 450 nm. A standard curve was prepared from seven human PGC-1 α standard dilutions, and human PGC-1 α sample concentration was determined [ 24 ]. All measurements were performed blinded with respect to clinical data.…”

Combined high-intensity interval training and green tea supplementation enhance metabolic and antioxidant status in response to acute exercise in overweight women

“…Treatment with flavonoid (green tea catechins) leads to increased mitochondrial biogenesis and increased energy expenditure in mice, possibly by SIRT1-mediated increase in PGC-1α activity [19,34]. It is also possible that the effects on BMI are caused by an influence of SIRT1 on appetite and energy intake, since SIRT1 is highly expressed in brain [4,6]. Previous studies showed that weight loss induces an increase in tissue and circulating SIRT1 levels in obese patients [4,5,13].…”

“…It is not clear how SIRT1 and PGC-1α influence BMI or Fat percentage, but potential mechanisms include a repressive effect on PPARc, central effects through satiety, increased energy expenditure, or effects by modification of Clock genes [4][5][6]. One limitation of the present study was lack of measurement of upstream and downstream factors of SIRT1 and PGC1α such as AMPK, calmodulin and PPARa, PPARy or measuring them via biopsies in humans to determine the exact signaling pathway of HIIT and green tea supplement in controlling weight.…”

“…Therefore, SIRT1 is potentially present in the extracellular component [ 4 , 5 ]. Although the main source of circulating SIRT1 is not known research results indicate that the negative metabolic effects of obesity could be related, at least in part, to the reduced levels of SIRT1 in the blood [ 6 , 7 ]. Moreover, what regulates circulating SIRT1 vs. tissue SIRT1 is still unknown.…”