Effect of electroacupuncture on gastric interstitial cells of Cajal in a rat model of diabetic gastroparesis

Lin, Guohua; Zhang, Jiawei; Li, Lixia; Zou, Zhuocheng; Chen, Chuyun; Xue, Lifei; Zhao, Lanfeng

doi:10.3892/etm.2016.3185

Cited by 15 publications

(5 citation statements)

References 20 publications

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“…Moreover, in the spatial structure, ICC-AP has also been found to have a closer relationship to the resident macrophages in muscularis [ 11 ]. It has been proposed that EA might help to reverse the pathological changes of ICC in diabetic rats [ 12 ]. Our previous study found the accelerative effects of gastric emptying by low- and high-frequency EA in diabetic rats; these may be attributed to the nearly unbroken ICC networks [ 2 ].…”

Section: Discussionmentioning

confidence: 99%

Electroacupuncture at ST‐36 Protects Interstitial Cells of Cajal via Sustaining Heme Oxygenase‐1 Positive M2 Macrophages in the Stomach of Diabetic Mice

Tian

Song

Zhu

et al. 2018

Oxidative Medicine and Cellular Longevity

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Background Electroacupuncture (EA) at ST-36 has been reported to improve delayed gastric emptying and protect the networks of ICC in diabetic models. However, the mechanisms of the effects of EA are still unclear. The purpose of this study was to investigate whether the HO-1 positive M2 macrophages participate in the protective effects of EA for the ICC networks. Methods Male C57BL/6 mice were randomized into five groups: the normal control group, diabetic group (DM), diabetic mice with sham EA group (SEA), diabetic mice with low frequency EA group (LEA), and diabetic mice with high frequency EA group (HEA). ICC network changes were detected by Ano1 immunostaining. F4/80 and HO-1 costaining was used to measure HO-1 positive macrophage expression. Western blot and PCR methods were applied to monitor HO-1, IL-10, and macrophage markers, respectively. The serum MDA levels were detected by a commercial kit. Results This study presents the following results: (1) Compared with the control group, ICC networks were severely disrupted in the DM group, but no obvious changes were found in the LEA and HEA groups. (2) Many HO-1 positive macrophages could be observed in the LEA and HEA groups, and the expression of HO-1 was also markedly upregulated. (3) The IL-10 expression was obviously upregulated in the LEA and HEA groups. (4) The serum MDA levels were decreased in the real EA group. (5) When compared to the DM group, the expression of CD163 and Arg-1 was increased in the LEA and HEA groups, but the iNOS expression was decreased. Conclusion The protective effects of EA on the networks of ICC may rely on the HO-1 positive macrophages to mediate anti-inflammatory and antioxidative stress effects.

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Section: Discussionmentioning

confidence: 99%

Electroacupuncture at ST‐36 Protects Interstitial Cells of Cajal via Sustaining Heme Oxygenase‐1 Positive M2 Macrophages in the Stomach of Diabetic Mice

Tian

Song

Zhu

et al. 2018

Oxidative Medicine and Cellular Longevity

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“…A reduced density of Interstitial cell of Cajal (ICC) is the most prominent cellular abnormality in both idiopathic and diabetic gastroparesis, and it provides a rational basis for the genesis of dysrhythmia in gastroparesis . EA was reported to reverse the pathological changes of gastric ICCs in a rodent model of diabetic gastroparesis …”

Section: Discussionmentioning

confidence: 99%

Efficacy of needleless transcutaneous electroacupuncture in synchronization with breathing for symptomatic idiopathic gastroparesis: A blinded and controlled acute treatment trial

Guan

Sun

Bashashati

et al. 2018

Neurogastroenterology Motil

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In sham or STEA, the nausea scores of 2.7 ± 0.5 and 1.9 ± 0.5 at fasting baseline, respectively, increased to 5.9 ± 0.4 and 5.8 ± 0.3 during Ensure test (P < .05, vs baseline), subsequently reduced to 3.4 ± 0.6 with sham or 3.6 ± 0.6 with STEA, respectively (P < .05, vs Ensure period). Experiments with sham and STEA started with similar % of normal waves on EGG (66.4 ± 3.9 and 61.8 ± 3.0, respectively); decreased to 63. 5 ± 4.1 and 58.2 ± 2.8 during the Ensure test. After STEA, there was ~24% increase in % of normal waves, significantly different from the sham (6.0%) (P < .01). In sham or STEA, vagal activity was identical at baseline and after the Ensure. STEA induced a 3-fold increase in vagal activity compared with sham (P < .01). Ensure increased serum PP levels, and both treatments decreased the PP CONCLUSIONS: In IGP, STEA is not superior to Sham in decreasing nausea, but is more effective in improving gastric dysrhythmia.

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“…During the 13 w of the experiment, the rats with random blood glucose level of ≤ 16.7 mmol/L were removed from the experiment. Success criteria of the DGP model were as follows [14]: the blood glucose level to be ≥ 16.7 mmol/L; the general conditions and stool characteristics of the rats to be significantly different from the blank group; the rate of gastric emptying and small intestinal propulsion rate in rats to be significantly different from the blank group.…”

Section: Modelling Methods and Evaluationmentioning

confidence: 99%

Study on the influence of acupoints selection on ghrelin and GHSR expression in gastric fundus tissue of DGP rats

Yue¹,

Li²,

Liu³

et al. 2018

biomedicalresearch

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Objective: To explore the acupoint selection compatibility according to different departments and to study the influence on ghrelin and GHSR expression in the gastric fundus in rats with Diabetic Gastroparesis (DGP). Methods: Sixty male SPF SD rats were given adaptive feeding for one week and were randomly divided into a blank control group, a model group, the Zusanli and Zhongwan (ZZ) group, the Zusanli and Neiguan group (ZN), as well as the Zusanli and non-meridian acupoint (ZNA) group, with 12 rats in each group. Except for the blank group, the other 48 rats were used to establish a DGP model by using streptozotocin. Acupuncture therapy was performed from the end of the tenth week for an additional four weeks. Then the rats were sacrificed and the serum and gastric fundus tissues were collected. ELISA assay was used to detect the ghrelin content in the serum. The expression of ghrelin positive cells in the gastric fundus tissue was detected by immunohistochemistry. The expression of ghrelin and GHSR mRNA in the gastric fundus tissue was detected by real-time PCR. Results: Compared with the blank group, the food intake and the expression of ghrelin and GHSR mRNA in the gastric fundus tissue in the model group were significantly increased, while the small intestinal propulsion rate, the ghrelin content in the serum and the gray value of the gastric fundus were significantly decreased (P<0.05). Compared with the model group, the expression of ghrelin and GHSR mRNA in the gastric fundus tissue, the small intestinal propulsion rate, the ghrelin content in the serum and the gray value of the gastric fundus were significantly increased (P<0.05). Compared with the ZZ group, the food intake were significantly increased in the ZN and the ZNA groups (P<0.05), and the expression of ghrelin and GHSR mRNA in the gastric fundus tissue of rats were significantly increased (P<0.05) but the ghrelin content in the serum in ZN group was significantly decreased (P<0.05). Conclusions: The improvement of gastrointestinal motility in DGP by acupuncture therapy was associated with the ghrelin content in the serum and GHSR expression in the gastric fundus. The treatment effect of local acupoints compatibility was better than that of the distal acupoints compatibility, and the acupoint selection according to department was one of the main influencing factors of acupoints compatibility.

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Effect of electroacupuncture on gastric interstitial cells of Cajal in a rat model of diabetic gastroparesis

Cited by 15 publications

References 20 publications

Electroacupuncture at ST‐36 Protects Interstitial Cells of Cajal via Sustaining Heme Oxygenase‐1 Positive M2 Macrophages in the Stomach of Diabetic Mice

Electroacupuncture at ST‐36 Protects Interstitial Cells of Cajal via Sustaining Heme Oxygenase‐1 Positive M2 Macrophages in the Stomach of Diabetic Mice

Efficacy of needleless transcutaneous electroacupuncture in synchronization with breathing for symptomatic idiopathic gastroparesis: A blinded and controlled acute treatment trial

Study on the influence of acupoints selection on ghrelin and GHSR expression in gastric fundus tissue of DGP rats

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