Effect of electrolyte and solvent composition on capillary electrophoretic separation of some pharmaceuticals in non-aqueous media

Porras, Simo P.; Valkó, István E.; Jyske, Pentti; Riekkola, Marja‐Liisa

doi:10.1016/s0165-022x(98)00031-1

Cited by 32 publications

(26 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…5) upon moving from methanol to 1-pentanol solution. The exceptional migration behavior of benzthiazide has previously been reported in electrolyte solutions containing methanol and acetonitrile with different electrolyte salts [28]. As with the basic analytes, the decreasing dielectric constant of the medium probably also has an effect on the effective mobilities of the acidic analytes.…”

Section: Basic Analytesmentioning

confidence: 75%

Alcohols and wide-bore capillaries in nonaqueous capillary electrophoresis

et al. 1999

Self Cite

View full text Add to dashboard Cite

Section: Basic Analytesmentioning

confidence: 75%

Alcohols and wide-bore capillaries in nonaqueous capillary electrophoresis

et al. 1999

Self Cite

View full text Add to dashboard Cite

“…The effect of alkali metal cations on the EOF has previously been studied by Porras et al [16] in BGEs based on methanol and ACN (1:1). They found that the decrease in EOF was in the order Li , i.e.…”

Section: Influence Of Alkali Metal Hydroxide On Eofmentioning

confidence: 99%

“…Porras et al [16] studied the effect of different alkali metal acetates on the electroosmosis in MeOH/ACN-based BGEs and observed a trend of decreasing electroosmosis with increasing crystal radius of the alkali metal cation. The same trend has also been observed in aqueous BGEs [17,18].…”

Section: Introductionmentioning

confidence: 99%

Effect of alkali metal hydroxides on the enantioseparation of amines using di-O-isopropylidene-keto-L-gulonic acid as the selector in NACE

et al. 2006

View full text Add to dashboard Cite

The present work demonstrates the importance of the ionic composition in the BGE for enantioseparation. (-)-2,3:4,6-di-O-Isopropylidene-2-keto-L-gulonic acid ((-)-DIKGA) has been used as the chiral selector in methanolic and ethanolic BGEs. The influence of added alkali metal hydroxides on the EOF and the chiral separation of amines (atenolol, isoprenaline, pindolol and propranolol) have been studied. The ion-pair formation constants in ethanol were determined by precision conductometry for the enantiomers of pindolol with (-)-DIKGA, for Li(+), Na(+) and Cs(+) with (-)-DIKGA, and also for the corresponding alkali metal hydroxides. The effective mobilities and the enantiomeric mobility differences were affected by the type of alkali metal hydroxide (LiOH, NaOH, KOH, RbOH or CsOH) added to the BGE. The effective mobility and mobility difference were increased with decrease in solvated radius of the alkali metal cation. These differences could partly be correlated to the ion-pair formation constants of the alkali metal cations with the chiral selector, affecting the equilibrium concentration of the free selector. The electroosmosis was also affected by the alkali metal hydroxide added to the BGE. The cathodic electroosmosis decreased with decreasing solvated radius of the alkali metal cation added to the BGE. Interestingly, the cathodic EOF was even reversed, i.e. became anodic in the ethanolic BGEs containing KOH, RbOH or CsOH and the methanolic ones with RbOH and CsOH.

show abstract

“…The interest has been nourished by the possibility of altering the separation parameters, such as resolution, analysis time, and selectivity, simply by modifying the composition of the background electrolyte (BGE) [24][25][26][27][28]. Many organic compounds are more soluble and stable in organic solvents than in aqueous solutions.…”

Section: Introductionmentioning

confidence: 99%

Separation of fluorescein isothiocyanate‐labeled amines by microchip electrophoresis in uncoated and polyvinyl alcohol‐coated glass chips using water and dimethyl sulfoxide as solvents of background electrolyte

et al. 2004

Self Cite

View full text Add to dashboard Cite

On-chip capillary electrophoresis with uncoated and polyvinyl alcohol-coated glass channels in aqueous and nonaqueous dimethyl sulfoxide (DMSO) background electrolyte (BGE) solutions was applied in the separation of five amines derivatized with fluorescein-5-isothiocyanate. In aqueous BGE at pH 9.2, baseline separation of the analytes was not achieved on uncoated glass chips, but the separation was clearly improved when the chip channels were coated with polyvinyl alcohol (PVA). Separation was successful in nonaqueous DMSO electrolyte solution containing ammonium acetate and sodium methoxide, on both uncoated and PVA-coated glass microchips. The differences in the pK(a) values of analytes were probably amplified in DMSO, and all five analytes were at least partly dissociated and were separated. Because the viscosity of DMSO is higher than that of water, the migration times were longer in DMSO.

show abstract

Effect of electrolyte and solvent composition on capillary electrophoretic separation of some pharmaceuticals in non-aqueous media

Cited by 32 publications

References 23 publications

Alcohols and wide-bore capillaries in nonaqueous capillary electrophoresis

Alcohols and wide-bore capillaries in nonaqueous capillary electrophoresis

Effect of alkali metal hydroxides on the enantioseparation of amines using di-O-isopropylidene-keto-L-gulonic acid as the selector in NACE

Separation of fluorescein isothiocyanate‐labeled amines by microchip electrophoresis in uncoated and polyvinyl alcohol‐coated glass chips using water and dimethyl sulfoxide as solvents of background electrolyte

Contact Info

Product

Resources

About