Effect of Enamel Matrix Derivative on Periodontal Wound Healing and Regeneration in an Osteoporotic Model

Miron, Richard J.; Wei, Lingfei; Yang, Shuang; Caluseru, Oana M.; Sculean, Anton; Zhang, Yufeng

doi:10.1902/jop.2014.130745

Cited by 14 publications

(14 citation statements)

References 43 publications

(94 reference statements)

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“…In addition, previous studies (1–3) strongly support the hypothesis that enamel matrix proteins (EMPs), known for their impact on the structural organization of tooth enamel, may serve an important role in periodontal tissue formation (1). It is believed that enamel matrix derivative (EMD), the active component of Emdogain (Straumann), has odontogenic effects through inducing the proliferation and differentiation of connective tissue progenitor cells, stimulating bone growth and arresting gingival epithelial cell migration (2,3). …”

Section: Introductionmentioning

confidence: 71%

Effects of enamel matrix proteins on adherence, proliferation and migration of epithelial cells: A real-time in vitro study

Wyganowska‐Świątkowska

Urbaniak

Lipinski

et al. 2016

Experimental and Therapeutic Medicine

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Enamel matrix derivative (EMD) can mimic odontogenic effects by inducing the proliferation and differentiation of connective tissue progenitor cells, stimulating bone growth and arresting epithelial cells migration. To the best of our knowledge, there is no data indicating that any active component of EMD reduces epithelial cell viability. The present study examines the impact of commercial lyophilized EMD, porcine recombinant amelogenin (prAMEL; 21.3 kDa) and tyrosine-rich amelogenin peptide (TRAP) on the adherence, proliferation and migration of human epithelial cells in real-time. The tongue carcinoma cell line SCC-25 was stimulated with EMD, porcine recombinant AMEL and TRAP, at concentrations of 12.5, 25 and 50 µg/ml. Cell adherence, migration and proliferation were monitored in real-time using the xCELLigence system. No significant effects of EMD on the morphology, adhesion, proliferation and migration of SCC-25 cells were observed. However, porcine recombinant AMEL had a dose-dependent inhibitory effect on SCC-25 cell proliferation and migration. Predominantly, no notable differences were found between control and TRAP-treated cells in terms of cell adhesion and migration, a decrease in proliferation was observed, but this was not statistically significant. EMD and its active components do not increase the tongue cancer cell viability.

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Section: Introductionmentioning

confidence: 71%

Effects of enamel matrix proteins on adherence, proliferation and migration of epithelial cells: A real-time in vitro study

Wyganowska‐Świątkowska

Urbaniak

Lipinski

et al. 2016

Experimental and Therapeutic Medicine

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“…The application of EMD on the grafted sites may have helped reduce inflammation and associated symptoms such as pain. EMD has been shown to play a significant role in wound healing favoring soft tissue regeneration and angiogenic activity . Indeed, EMD sold under the trademark Xelma ® showed the potential therapeutic uses of this protein for the treatment of “hard to heal” ulcers such as diabetic foot ulcers and rheumatoid disease .…”

Section: Discussionmentioning

confidence: 99%

“…EMD has been shown to play a significant role in wound healing favoring soft tissue regeneration and angiogenic activity. 44 Indeed, EMD sold under the trademark Xelma ® showed the potential therapeutic uses of this protein for the treatment of "hard to heal" ulcers such as diabetic foot ulcers and rheumatoid disease. [45][46][47] With regard to patient satisfaction with the provided treatment, although the results did not reach statistical significance, there was a trend toward more patients in group 1 answering "Yes" to the question "Will you have the treatment again?"…”

Section: Discussionmentioning

confidence: 99%

A 3‐year prospective clinical and patient‐centered trial on subepithelial connective tissue graft with or without enamel matrix derivative in Class I‐II Miller recessions

Mercado

Hamlet

Ivanovski

2019

J of Periodontal Research

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Background and Objective The study compared clinical and patient‐centered outcomes of subepithelial connective tissue graft (CTG) with and without enamel matrix derivative (EMD) in the treatment of Class I‐II Miller periodontal recession defects. Material and Methods This prospective clinical study evaluated 80 patients over a 3 years follow‐up in a private periodontal practice. A total of 144 maxillary and mandibular anterior teeth were divided into two groups: group 1 (CTG with EMD—80 teeth) and group 2 (CTG only—64 teeth). Recession (REC), keratinized tissue (KT) width, % root overage, patient‐centered outcomes, and pain visual analog scale (P‐VAS) were compared between the two groups. Results At 3 years follow‐up at a patient level, statistically significant changes in REC were achieved in both group 1 (4.65 ± 1.84 to 0.39 ± 0.19 mm) and group 2 (4.43 ± 1.11 to 0.92 ± 0.43 mm). Complete root coverage (CRC) was achieved in 66.4% of group 1 and 50.1% of group 2. At both patient and tooth level, the 3‐year outcomes were superior for group 1 compared with group 2 in terms of % root coverage, REC, and KT width. Clinical attachment loss (CAL) was reduced in group 1 compared with group 2 at the tooth level analysis only (<.01). Significantly less pain was reported using the pain visual analog Scale (P < .001) at the two weeks follow‐up post‐surgery in group 1. Conclusions Addition of EMD results in improved root coverage outcomes and higher amounts of keratinized tissue width 36 months after treatment of multiple adjacent recessions on maxillary and mandibular anterior teeth. The adjunctive use of EMD also resulted in significantly reduced pain 14 days after the surgery.

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“…All animals were allowed to acclimate to the laboratory environment for 1 week before surgical procedures were carried out. For 32 of the 64 animals, ovariectomy (OVX) surgery was performed at 8 weeks of age as previously described [18,19]. Briefly, general anesthesia was achieved by intraperitoneal injection of chloral hydrate (10 %, 4 ml/kg body weight); the rats were operated with a 10-mm linear bilateral lumbar lateral skin incisions.…”

Section: Animal Experimentsmentioning

confidence: 99%

Initial changes in alveolar bone volume for sham-operated and ovariectomized rats in ligature-induced experimental periodontitis

Dai

Shi

et al. 2015

Clin Oral Invest

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Effect of Enamel Matrix Derivative on Periodontal Wound Healing and Regeneration in an Osteoporotic Model

Cited by 14 publications

References 43 publications

Effects of enamel matrix proteins on adherence, proliferation and migration of epithelial cells: A real-time in vitro study

Effects of enamel matrix proteins on adherence, proliferation and migration of epithelial cells: A real-time in vitro study

A 3‐year prospective clinical and patient‐centered trial on subepithelial connective tissue graft with or without enamel matrix derivative in Class I‐II Miller recessions

Initial changes in alveolar bone volume for sham-operated and ovariectomized rats in ligature-induced experimental periodontitis

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