Effect of endogenous phosphoenolpyruvate potential on fluoride inhibition of glucose uptake by Streptococcus mutans

Germaine, G R; Tellefson, L M

doi:10.1128/iai.51.1.119-124.1986

Cited by 8 publications

(2 citation statements)

References 22 publications

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“…). There are many published reports concerning the effects of fluoride on Streptococcus; these include inhibition of enolase (a glycolytic enzyme) and the PEP–PTS pathway and enhancement of intracellular acidification and subsequent impairment of the entire glycolytic metabolisms . In contrast, the mechanism(s) by which fluoride inhibits Actinomyces is still unclear.…”

Section: Discussionmentioning

confidence: 99%

Fluoride‐sensitivity of growth and acid production of oral Actinomyces: comparison with oral Streptococcus

Kawashima

Nakajo²,

Washio³

et al. 2013

Microbiology and Immunology

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Actinomyces are predominant oral bacteria; however, their cariogenic potential in terms of acid production and fluoride sensitivity has not been elucidated in detail and compared with that of other caries-associated oral bacteria, such as Streptococcus. Therefore, this study aimed to elucidate and compare the acid production and growth of Actinomyces and Streptococcus in the presence of bicarbonate and fluoride to mimic conditions in the oral cavity. Acid production from glucose was measured by pHstat at pH 5.5 and 7.0 under anaerobic conditions. Growth rate was assessed by optical density in anaerobic culture. Although Actinomyces produced acid at a lower rate than did Streptococcus, their acid production was more tolerant of fluoride (ID acid production 50 ¼ 110-170 ppm at pH 7.0 and 10-13 ppm at pH 5.5) than that of Streptococcus (ID acid production 50 ¼ 36-53 ppm at pH 7.0 and 6.3-6.5 ppm at pH 5.5). Bicarbonate increased acid production by Actinomyces with prominent succinate production and enhanced their fluoride tolerance (ID acid production 50 ¼ 220-320 ppm at pH 7.0 and 33-52 ppm at pH 5.5). Bicarbonate had no effect on these variables in Streptococcus. In addition, although the growth rate of Actinomyces was lower than that of Streptococcus, Actinomyces growth was more tolerant of fluoride (ID growth 50 ¼ 130-160 ppm) than was that of Streptococcus (ID growth 50 ¼ 27-36 ppm). These results indicate that oral Actinomyces are more tolerant of fluoride than oral Streptococcus, and bicarbonate enhances the fluoride tolerance of oral Actinomyces. Because of the limited number of species tested here, further study is needed to generalize these findings to the genus level.Key words acid production, bicarbonate, fluoride, oral Actinomyces.Similarly to Streptococcus, Actinomyces are predominant oral bacteria (1) that are detected from dental plaque biofilm of healthy tooth surfaces and oral mucosa (2). This bacterium is also frequently isolated from periodontitis lesions (3-6) and root surface caries (7-10), suggesting that it is associated with various oral diseases. In particular, its role in the causation of root surface caries has been a focus (7,11). The importance of this research has further been supported by recent comprehensive analyses of microbiota in root surface caries lesions (12)(13)(14).The main cariogenic factor is acid production by carbohydrate metabolism. Actinomyces species can degrade List of Abbreviations: A., Actinomyces; ATP, adenosine-5 0 -triphosphate; EMP, Embden-Meyerhof-Parnas; G3PDH, glyceraldehyde-3-phosphate dehydrogenase; HF, hydrogen fluoride; ID acid production 50, 50% inhibitory dose of fluoride on acid production; ID growth 50, 50% inhibitory dose of fluoride on growth; LDH, lactate dehydrogenase; NAD, nicotinamide adenine dinucleotide (oxidized form); NADH, nicotinamide adenine dinucleotide (reduced form); OD, optical density; PEP, phosphoenolpyruvate; PEP-PTS, phosphoenolpyruvate-dependent phosphotransferase; PFL, pyruvate formate-lyase; PPB, potassium phosphate b...

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Section: Discussionmentioning

confidence: 99%

Fluoride‐sensitivity of growth and acid production of oral Actinomyces: comparison with oral Streptococcus

Kawashima

Nakajo²,

Washio³

et al. 2013

Microbiology and Immunology

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“…F luoride is recognized as a stimulator of bone recalcification (Krokowski, 1984). Fluoride released from glass-ionomer materials may prevent caries (Dhondt et al, 2001) due to the inhibition of glucose uptake by bacteria (Germaine and Tellefson, 1986). Fluoride has shown considerable variation in respect of cytotoxicity against different cultured cells (Carlson and Suttie, 1967;Hongslo et al, 1974;Oguro et al, 1983).…”

Section: Introductionmentioning

confidence: 99%

Possible Link between Glycolysis and Apoptosis Induced by Sodium Fluoride

et al. 2005

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Fluoride has been used to prevent caries in the dentition, but the possible underlying mechanisms of cytotoxicity induction by this compound are still unclear. Since fluoride is known as an inhibitor of glycolytic enzymes, we investigated the possible connection between NaF-induced apoptosis and glycolysis in human promyelocytic leukemia HL-60 cells. NaF-induced apoptotic cell death is characterized by caspase activation, internucleosomal DNA fragmentation, loss of mitochondrial membrane potential, and production of apoptotic bodies. Higher activation of caspases-3 and -9, as compared with that of caspase-8, suggested the involvement of an extrinsic pathway. Utilization of glucose was nearly halted by NaF, whereas that of glutamine was rather enhanced. NaF enhanced the expression of Bad protein, but not that of Bcl-2 and Bax proteins, and reduced HIF-1alpha mRNA expression. Analysis of these data suggests a possible link between glycolysis and apoptosis.

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Biochemistry of Inorganic Fluoride

Kirk

1991

Biochemistry of the Elemental Halogens and Inorganic Halides

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Effect of endogenous phosphoenolpyruvate potential on fluoride inhibition of glucose uptake by Streptococcus mutans

Cited by 8 publications

References 22 publications

Fluoride‐sensitivity of growth and acid production of oral Actinomyces: comparison with oral Streptococcus

Fluoride‐sensitivity of growth and acid production of oral Actinomyces: comparison with oral Streptococcus

Possible Link between Glycolysis and Apoptosis Induced by Sodium Fluoride

Biochemistry of Inorganic Fluoride

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