Effect of endothelin and endothelin receptor blockade on capillary permeability in experimental pancreatitis

Eibl, Guido; Hotz, Hubert G.; Faulhaber, Jörg; Kirchengast, Michael; Buhr, H. J.; Foitzik, T.

doi:10.1136/gut.46.3.390

Cited by 66 publications

(54 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the model of severe AP used in this study, selective blockade of endothelin A receptors by LU-135252 has been shown to increase capillary blood flow and decrease permeability both inside and outside the pancreas. These effects were also present but significantly diminished with nonselective endothelin A and B receptor blockade [16,17].…”

Section: Methodsmentioning

confidence: 99%

Effect of Fluid Resuscitation with and without Endothelin A Receptor Blockade on Hemoconcentration and Organ Function in Experimental Pancreatitis

Forgács

Eibl²,

Faulhaber³

et al. 2000

Eur Surg Res

View full text Add to dashboard Cite

Background: Intravascular fluid loss contributes to pancreatitis-associated multiple organ dysfunction and is thus a major target for therapy in this life-threatening disease. Aim: To evaluate intravascular fluid loss and extravascular fluid sequestration together with cardiorespiratory and renal function in a well-established rat model of severe acute pancreatitis (AP) and to investigate the effect of fluid resuscitation with and without endothelin receptor A blockade on these parameters. Method: Induction of AP in rats by a standardized bile salt infusion into the pancreatic duct and intravenous cerulein hyperstimulation. Six hours after AP induction, animals were randomized into 4 groups to receive (1) no therapy; (2) 4 ml/kg/h Ringer’s lactate (RL) i.v.; (3) 8 ml/kg/h RL i.v., or (4) 4 ml/kg/h RL plus an endothelin receptor antagonist. Target parameters measured before and after AP induction and during the 24-hour observation period included: mean arterial blood pressure, heart rate, hematocrit, arterial blood gases, urine production, ascites and pleural effusions. Results: After 6 h, all animals presented with severe hemoconcentration (hematocrit >57%) and oliguria (<0.5 ml/6 h). Cardiorespiratory parameters were within the normal range. Up to 12 h after AP induction, animals without therapy had an increased hematocrit and oliguria and developed metabolic acidosis. Animals receiving fluid resuscitation had a significant drop in hematocrit and maintained compensated blood gas values. A significant increase in urine production was only observed in animals given 8 mg/kg/h RL. Between 12 and 24 h, urine production significantly increased with fluid resuscitation and respiratory parameters stabilized except for animals treated with 8 ml/kg/h RL which developed arterial hypoxia and hypercapnia. Conclusions: Intravascular fluid loss and extravascular fluid sequestration together with decreased urine production characterize the early phase of this model of severe AP. Massive fluid resuscitation necessary for increasing urine output may lead to respiratory distress. Reduction of intravascular fluid loss by endothelin receptor blockade is associated with improved renal and respiratory function.

show abstract

Section: Methodsmentioning

confidence: 99%

Effect of Fluid Resuscitation with and without Endothelin A Receptor Blockade on Hemoconcentration and Organ Function in Experimental Pancreatitis

Forgács

Eibl²,

Faulhaber³

et al. 2000

Eur Surg Res

View full text Add to dashboard Cite

show abstract

“…[28]. In this study, at all time points after operation, blood viscosity parameters determined in model control group were obviously greater than those in sham-operated group (P<0.01 or P<0.001), indicating that hemorheological abnormality and microcirculation disturbance are present in SAP.…”

Section: Discussionmentioning

confidence: 44%

Effect of Baicalin on Inflammatory Mediator Levels and Microcirculation Disturbance in Rats With Severe Acute Pancreatitis

Zhang

Tian

et al. 2009

Pancreas

View full text Add to dashboard Cite

AbstractsObjective: To investigate the effect of Bacailin on inflammatory mediator levels and microcirculation disturbance in severe acute pancreatitis (SAP) rats and explore its therapeutic mechanism on this disease. Methods: SAP model rats were randomly divided into model control group and Baicalin treated group, 45 rats in each group. The same number of normal rats were included in sham-operated group. These groups were further subdivided into 3 h, 6 h and 12 h subgroups, respectively (15 rats in each subgroup). At 3, 6 and 12 hours after operation, rats were killed to conduct the following experiments: (1) to examine the mortality rates of rats, the ascites volume and pancreatic pathological changes in each group; (2) to determine the contents of amylase, PLA 2 , TXB 2 , PGE 2 , PAF and IL-1β in blood as well as the changes in blood viscosity.Results: (1) Compared to model control group, treatment with Baicalin is able to improve the pathological damage of the pancreas, lower the contents of amylase and multiple inflammatory mediators in blood, decrease the amount of ascitic fluid and reduce the mortality rates of SAP rats (P<0.01, P<0.01 or P<0.001); (2) at 3 hours after operation, the low-shear whole blood viscosity (1/s)(mPa.s) in Baicalin treated group was significantly lower than that in model control group (P<0.01); at 12 hours after operation, both the high-shear (200/s) (mPa.s) and low-shear whole blood viscosity (1/s) (mPa.s) in Baicalin treated group were also significantly lower than those in model control group (P<0.01 for all). Conclusion: Baicalin, as a new drug, has good prospects in the treatment of SAP since it can exert therapeutic effects on this disease through inhibiting the production of inflammatory mediators, lowering blood viscosity, improving microcirculation and mitigating the pathological damage of the pancreas.

show abstract

“…This method is safety and noninvasive, but it is expensive and is not adapt to popularization and application in clinic and basic study. 2) Fluorescein labeled albumin: albumin is fluorescence labeled via intravenous fluorescent, and leakage rate is estimated using intensity changes of relative fluorescence of the blood vessels inside and outside using fluorescence microscope 22,23 . In this study, rhodamine isothiocyanate is used to label bovine serum albumin to form RBITC-BSA, which is used to estimate leakage rate via observing leakage phenomenon of microvascular albumin in postoperative rabbit abdominal mesenteric.…”

Section: ■ Discussionmentioning

confidence: 99%

Impacts of albumin synergized with hydroxyethyl starch on early microvascular albumin leakage after major abdominal surgery in rabbits

Cen

et al. 2017

Acta Cir. Bras.

View full text Add to dashboard Cite

Impacts of albumin synergized with hydroxyethyl starch on early microvascular albumin leakage after major abdominal surgery in rabbits 1 3-Experimental SurgeryActa Cir Bras. 2017;32(2):108-115 AbstractPurpose: To investigate the impacts of albumin synergized with hydroxyethyl starch (HES) on early microvascular albumin leakage after major abdominal surgery in rabbits. Methods: Forty male Japanese rabbits were randomly divided into four groups: the control group, the saline group, the albumin group, and the Syn group (hydroxyethyl starch+albumin). The latter three groups were performed gastrectomy plus resection of pancreatic body and tail and splenectomy. The serum albumin concentration was detected before and 48h after surgery, and the conditions of mesenteric microvascular leakage in these 4 groups were observed under microscope 48 h after surgery to calculate the leakage rate. Results: Compared with the saline group, the albumin group and the Syn group exhibited significantly increased serum albumin concentrations 48h after surgery (P<0.05). The albumin leakage rate was the most obvious in the albumin group, followed by the saline group, while that in the Syn group was the minimal, and there existed significant differences among these groups (P<0.05). Conclusion:Simple administration of albumin in the early stage after major abdominal surgery could increase the albumin leakage, while the synergization of albumin and hydroxyethyl starch could reduce the albumin leakage.

show abstract

Effect of endothelin and endothelin receptor blockade on capillary permeability in experimental pancreatitis

Cited by 66 publications

References 26 publications

Effect of Fluid Resuscitation with and without Endothelin A Receptor Blockade on Hemoconcentration and Organ Function in Experimental Pancreatitis

Effect of Fluid Resuscitation with and without Endothelin A Receptor Blockade on Hemoconcentration and Organ Function in Experimental Pancreatitis

Effect of Baicalin on Inflammatory Mediator Levels and Microcirculation Disturbance in Rats With Severe Acute Pancreatitis

Impacts of albumin synergized with hydroxyethyl starch on early microvascular albumin leakage after major abdominal surgery in rabbits

Contact Info

Product

Resources

About