Effect of Enterococcus faecalis OG1RF on human calvarial osteoblast apoptosis

Li, Yang; Sun, Shaoli; Wen, Cheng; Zhong, Jialin; Jiang, Qianzhou

doi:10.1186/s12903-022-02295-y

Cited by 3 publications

(1 citation statement)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Recently, a polymerase chain reaction (PCR) array analysis of 84 apoptosis-related genes in E. faecalis -infected human calvarial osteoblasts revealed that Bcl-2 family members acted as regulators of osteoblast apoptosis. Therefore, Bcl-2 family members may be potential therapeutic targets for RAP [ 110 ]. Notably, utilising inhibitors targeting pyroptosis or necroptosis-related molecules may have a significant impact on the resolution of E. faecalis -induced RAP since it reduces pro-inflammatory cytokine release rather than just inhibits cell death.…”

Section: E Faecalis -Modulated Host Cell Responsesmentioning

confidence: 99%

Role of Enterococcus faecalis in refractory apical periodontitis: from pathogenicity to host cell response

Deng

Lin

Liu

et al. 2023

Journal of Oral Microbiology

View full text Add to dashboard Cite

Background: Refractory apical periodontitis (RAP) is an oral infectious disease characterised by persistent inflammation, progressive alveolar bone destruction, and delayed bone healing. RAP has received increasing attention, because it cannot be cured after repeated root canal therapies. The aetiology of RAP is related to the complex interplay between the pathogen and its host. However, the exact pathogenesis of RAP remains unclarified and includes several factors, such as microorganism immunogenicity, host immunity and inflammation, and tissue destruction and repair. Enterococcus faecalis is the dominant pathogen involved in RAP, and has evolved multiple strategies to ensure survival, which cause persistent intraradicular and extraradicular infections. Objective: To review the crucial role of E. faecalis in the pathogenesis of RAP, and open new avenues for prevention and treatment of RAP. Methods: The PubMed and Web of Science databases were searched for pertinent publications, employing the search terms “Enterococcus faecalis”, “refractory apical periodontitis”, “persistent periapical periodontitis”, “pathogenicity”, “virulence”, “biofilm formation”, “dentine tubule”, “immune cell”, “macrophage”, and “osteoblast”. Results and Conclusion: Besides its high pathogenicity due to various virulence mechanisms, E. faecalis modulates the macrophage and osteoblast responses, including regulated cell death, cell polarisation, cell differentiation, and inflammatory response. An in-depth understanding of the multifaceted host cell responses modulated by E. faecalis will help to design potential future therapeutic strategies and overcome the challenges of sustained infection and delayed tissue healing in RAP.

show abstract

Section: E Faecalis -Modulated Host Cell Responsesmentioning

confidence: 99%

Role of Enterococcus faecalis in refractory apical periodontitis: from pathogenicity to host cell response

Deng

Lin

Liu

et al. 2023

Journal of Oral Microbiology

View full text Add to dashboard Cite

show abstract

A rat model establishment of bronchopulmonary dysplasia-related lung & brain injury within 28 days after birth

Lin,

Zhou,

Wang

2024

BMC Neurosci

View full text Add to dashboard Cite

Purpose Lung injury associated with bronchopulmonary dysplasia (BPD) and its related neurodevelopmental disorders have garnered increasing attention in the context of premature infants. Establishing a reliable animal model is essential for delving into the underlying mechanisms of these conditions. Methods Newborn rats were randomly assigned to two groups: the hyperoxia-induced BPD group and the normoxia (NO) group. For the BPD group, they were nurtured in a hyperoxic environment with a high oxygen inspired fraction (0.85) from birth until day 14 within 28 days postnatally. In contrast, the NO group consisted of newborn rats that were nurtured in a normoxic environment with a standard oxygen inspired fraction (0.21) for 28 days postnatally. Various pathological sections of both lung and brain tissues were examined. TUNEL staining, immunofluorescence assays, and functional tests were performed, and the results were meticulously analyzed to assess the impact of hyperoxia environments on the developing organs. Results In the newborn rats of the BPD group, a significant reduction in alveolar number coupled with enlargement was observed, alongside severe fibrosis, collagen deposition, and constriction of bronchi and vascular lumens. This was accompanied by an accumulation of inflammatory cells and a marked deterioration in lung function compared to the NO group ( P < 0.05 ). Additionally, a decrease in neuronal count, an increase in neuronal apoptosis, proliferation of neuroglia cells, and demyelination were noted, and poorer performance in the Morris water maze test within the BPD group ( P < 0.05 ). Conclusion The BPD-rats model was established successfully. Lung injury in the BPD group evident across the bronchi to the alveoli and pulmonary vessels, which was associated with deteriorated lung function at postnatal day 14. Concurrently, brain injury extended from the cerebral cortex to the hippocampus, which was associated with impaired performance in orientation navigation and spatial probe tests at postnatal day 28. Supplementary Information The online version contains supplementary material available at 10.1186/s12868-024-00912-w.

show abstract

Exploring the potential link between MitoEVs and the immune microenvironment of periodontitis based on machine learning and bioinformatics methods

Yang,

Zhao,

Chen

et al. 2024

BMC Oral Health

View full text Add to dashboard Cite

Background Periodontitis is a chronic inflammatory condition triggered by immune system malfunction. Mitochondrial extracellular vesicles (MitoEVs) are a group of highly heterogeneous extracellular vesicles (EVs) enriched in mitochondrial fractions. The objective of this research was to examine the correlation between MitoEVs and the immune microenvironment of periodontitis. Methods Data from MitoCarta 3.0, GeneCards, and GEO databases were utilized to identify differentially expressed MitoEV-related genes (MERGs) and conduct functional enrichment and pathway analyses. The random forest and LASSO algorithms were employed to identify hub MERGs. Infiltration levels of immune cells in periodontitis and healthy groups were estimated using the CIBERSORT algorithm, and phenotypic subgroups of periodontitis based on hub MERG expression levels were explored using a consensus clustering method. Results A total of 44 differentially expressed MERGs were identified. The random forest and LASSO algorithms identified 9 hub MERGs (BCL2L11, GLDC, CYP24A1, COQ2, MTPAP, NIPSNAP3A, FAM162A, MYO19, and NDUFS1). ROC curve analysis showed that the hub gene and logistic regression model presented excellent diagnostic and discriminating abilities. Immune infiltration and consensus clustering analysis indicated that hub MERGs were highly correlated with various types of immune cells, and there were significant differences in immune cells and hub MERGs among different periodontitis subtypes. Conclusion The periodontitis classification model based on MERGs shows excellent performance and can offer novel perspectives into the pathogenesis of periodontitis. The high correlation between MERGs and various immune cells and the significant differences between immune cells and MERGs in different periodontitis subtypes can clarify the regulatory roles of MitoEVs in the immune microenvironment of periodontitis. Future research should focus on elucidating the functional mechanisms of hub MERGs and exploring potential therapeutic interventions based on these findings.

show abstract

Effect of Enterococcus faecalis OG1RF on human calvarial osteoblast apoptosis

Cited by 3 publications

References 45 publications

Role of Enterococcus faecalis in refractory apical periodontitis: from pathogenicity to host cell response

Role of Enterococcus faecalis in refractory apical periodontitis: from pathogenicity to host cell response

A rat model establishment of bronchopulmonary dysplasia-related lung & brain injury within 28 days after birth

Exploring the potential link between MitoEVs and the immune microenvironment of periodontitis based on machine learning and bioinformatics methods

Contact Info

Product

Resources

About