Effect of Epidermal Growth Factor upon Morphological Changes of Human Lens Epithelial Cells

Abstract

Phase-contrast and transmission electron microscopic studies were performed to evaluate the effect of epidermal growth factor (EGF) upon the morphology of lens epithelial cells (LECs) obtained from human cataractous lenses, cultured on human anterior lens capsules, and divided into the following three groups: group 1 receiving no EGF supplement, group 2 supplemented with 1 ng/ml EGF, and group 3 supplemented with 10 ng/ml EGF. Phase-contrast microscopy revealed that the cells in the group supplemented with 1 ng/ml EGF concentration had elogated, and those supplemented with 10 ng/ml EGF disclosed changes indicating fibroblast-like cells. Under transmission electron microscopy, the cells in the group supplemented with 1 ng/ml EGF concentration had become multilayered and a few cells had a ball-and-socket junction structure and nucleolar chromatin condensation, while those supplemented with 10 ng/ml EGF showed changes not only in the cells but also in the anterior lens capsules to present numerous microfibers. This is the first study to confirm the effect of EGF upon LECs cultured on anterior lens capsules, a condition closely resembling the intraocular environment, and these findings are significant.

Histological and Biochemical Findings in Membranous Cataract

“…FGF and EGF promote the lens epithelial cell proliferation and induce multilayering, fibrous metaplasia and plaque formation [6,7] . Moreover, FGF and EGF stimulate fiber differentiation in the human lens in vitro and especially FGF plays a key role in the formation of posterior capsular opacification [8,9] .…”