Effect of erythorbic acid administration on ascorbic acid content in guinea pig tissues.

Abstract

SummaryThe effect of erythorbic acid (ErA) on ascorbic acid (AsA) content in the tissues of normal and AsA-deficient guinea pigs was studied . The animals were sacrificed at varying intervals during the experimental period, and the liver, adrenal glands, spleen and kidneys were removed. The amounts of AsA and ErA in the tissues were measured by HPLC. The content of AsA in the tissues of the animals administered both AsA and ErA was lower than that of the animals administered only AsA. But the disappearance rate of AsA from the tissues of the AsA-deficient animals was similar to that of the animals administered only ErA. The amount of AsA in the tissues of the animals administered both AsA and ErA during the repletion period was lower than that of the animals administered only AsA. These results suggest that ErA administration may affect the amount of AsA in the tissues by inhibiting its tissue uptake or its storage in the tissues, and not by accerelating the catabolism of AsA in the tissues.

Vitamin C Activity of Erythorbic Acid in Ascorbic Acid-Deficient Guinea Pigs.

“…Determination of AsA and ErA contents. The contents of AsA and ErA in the liver and adrenal glands were simultaneously determined by using HPLC, as described in our previous paper (4).…”

The Regulatory Effect of Ascorbate on the Carmnitine Synthesis in Primary Cultured Guinea Pig Hepatocytes.

“…On the other hand, ErA led to lower carnitine contents in cells than AsA. Arakawa et al (20) observed that when guinea pigs were orally supplemented with ErA at 20 times the AsA dose, the contents of ErA in liver were only about 20% of AsA. The rate of loss of ErA in tissues of the guinea pig was reported to be more rapid than that of AsA (21).…”

Erythorbic acid content in tissues of guinea pigs administered erythorbic acid.

“…Male albino guinea pigs (Hartley strain) with initial body weights of about 220g were used. All animals were housed individually in wire cages, and maintained on the AsA-deficient diet descrided previously (5) . Water and diets were offered ad libitum, and their body weights were recorded daily .…”

“…The AsA and ErA contents in the tissues were simultaneously determined using HPLC as described previously (5) .…”

“…The workers described above did not measure ErA and AsA separately in the tissues, since at that time there was no reliable, separative determination method for AsA and ErA. However, in our previous study we determined ErA and AsA simultaneously in the tissues of guinea pigs administered both AsA and ErA using high-performance liquid chromatography (HPLC) (5). Furthermore , we found that a large amount of ErA administered to guinea pigs for 30 days prevented the occurrence of scurvy and a complete recovery of body weight was seen when ErA was administered to AsA-deficient guinea pigs (5).…”