Effect of erythrocyte-sperm separation medium on nuclear, acrosomal, and membrane maturity parameters in human sperm

Soygur, Bikem; Celik, Soner; Çelik-Özenci, Çiler; Satı, Leyla

doi:10.1007/s10815-017-1085-1

Cited by 7 publications

(10 citation statements)

References 56 publications

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“…Although there has been no study with accepted protocols on the effect of ELB on either sperm parameters or DNA fragmentation, Soygur and associates found that ELB treatment in ejaculated spermatozoa had no detrimental effects on sperm parameters and DNA fragmentation. The exposure time in their study was set at only 5–10 s (Soygur et al., 2018).…”

Section: Discussionmentioning

confidence: 99%

“…The slides were examined by × 1,000 magnification with a light microscope and counting 200 spermatozoa. Unstained or pale blue‐stained cells were considered as normal, and dark blue cells deemed to be abnormal spermatozoa (Soygur et al., 2018). The percentage of abnormal spermatozoa was reported.…”

Section: Methodsmentioning

confidence: 99%

“…Nagy et al evaluated seven cases that were treated with ELB and reported no adverse effect on fertilisation and development rates (Nagy et al., 1997). A recent study focused on the safety of ELB, but their protocol used only 5–10 s of incubation (Soygur, Celik, Celik‐Ozenci, & Sati, 2018). This protocol does not follow the generally 5–10 min of ELB incubation used in many other studies (Esteves & Varghese, 2012; Nagy et al., 1997; Greta Verheyen et al., 2017).…”

Section: Introductionmentioning

confidence: 99%

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Application of erythrocyte lysing buffer (ELB) has detrimental effects on human sperm quality parameters, DNA fragmentation and chromatin structure

et al. 2020

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Over 70 million couples worldwide suffer from infertility (Ombelet et al., 2008). Approximately 1% of all infertile men have obstructive (OA) or nonobstructive azoospermia (NOA; Inhorn & Patrizio, 2015). Contributing to this statistic is the fact that azoospermia is characterised by a lack of spermatozoa in the ejaculated fluid (Fang et al., 2019). If viable testicular spermatozoa can be obtained from these patients, full developmental potential zygotes may be formed using the ICSI procedure (Fang et al., 2019; Shrem et al., 2019). Fortunately, tissue extracted from the testis contains spermatozoa and round germ cells, interstitial cells, Sertoli cells (Crabbé, Verheyen, Tournaye, & Van Steirteghem, 1997) and a high concentration of erythrocytes (Nagy, Verheyen, Tournaye, Devroey, & Van Steirteghem, 1997). According to commonly accepted testicular sperm preparation protocols, sperm suspension is exposed to erythrocyte lysing buffer (ELB) for 5-10 min. This preparation eliminates erythrocytes and produces a clean suspension of testicular spermatozoa. Therefore, it facilitates the search for sperm cells

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Application of erythrocyte lysing buffer (ELB) has detrimental effects on human sperm quality parameters, DNA fragmentation and chromatin structure

et al. 2020

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“…Several protocols have been implemented to optimize semen sampling, especially by removing RBC prior to freezing. Such protocols may be based on the application of density gradient centrifugation, erythrocyte-sperm separation media, and microfluidic systems [58][59][60]. The efficacy of gradient centrifugation was confirmed by the successful separation of viable motile canine spermatozoa from dead immotile spermatozoa and RBC using four different types of density gradient centrifugation media [58].…”

Section: Freezability Of Epididymal Semenmentioning

confidence: 97%

Canine and Feline Epididymal Semen—A Plentiful Source of Gametes

Hassan

Domain

Luvoni

et al. 2021

Animals

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Canine and feline epididymal semen provide an additional source of gametes to preserve the genetics of valuable breeding dogs and tomcats, especially for those that fail to ejaculate, need castration as a therapy or die unexpectedly. Moreover, since it is quite common to perform castration of non-breeding dogs and cats, the development of a gene bank of epididymal semen collected after castration would greatly contribute to increase the genetic diversity in dogs and cats. Collection and cryopreservation of epididymal semen necessitates a full understanding of the function of the epididymis and of the characteristics of epididymal spermatozoa as opposed to ejaculated semen. During collection of epididymal semen, specific factors may have a negative effect on epididymal semen quality and freezability. Accordingly, the elimination of these triggers could enhance epididymal semen freezability and consequently positively influence post-thaw semen quality and outcome for different ARTs.

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“…In addition, osmotic stress caused by the chemical structure of this buffer may damage the plasma membrane and alter sperm metabolism [ 21 ]. Conversely, Soygur et al showed that ELB itself and the cellular stress caused by erythrocyte lysis did not have detrimental effects on the survival of ejaculated sperm [ 22 ]. According to their protocol, however, ELB incubation was only allowed for 5–10 s, so there may not have been enough time for damage to occur.…”

Section: Processing and Selection Of Surgically Retrieved Sperm For Icsimentioning

confidence: 99%

Sperm Selection Procedures for Optimizing the Outcome of ICSI in Patients with NOA

Aydos

2021

JCM

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Retrieving spermatozoa from the testicles has been a great hope for patients with non-obstructive azoospermia (NOA), but relevant methods have not yet been developed to the level necessary to provide resolutions for all cases of NOA. Although performing testicular sperm extraction under microscopic magnification has increased sperm retrieval rates, in vitro selection and processing of quality sperm plays an essential role in the success of in vitro fertilization. Moreover, sperm cryopreservation is widely used in assisted reproductive technologies, whether for therapeutic purposes or for future fertility preservation. In recent years, there have been new developments using advanced technologies to freeze and preserve even very small numbers of sperm for which conventional techniques are inadequate. The present review provides an up-to-date summary of current strategies for maximizing sperm recovery from surgically obtained testicular samples and, as an extension, optimization of in vitro sperm processing techniques in the management of NOA.

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Effect of erythrocyte-sperm separation medium on nuclear, acrosomal, and membrane maturity parameters in human sperm

Abstract: ESSM can enhance the efficiency of sperm retrieval protocol and can also decrease the time required to collect spermatozoa while not affecting sperm morphogenetic properties.

Cited by 7 publications

References 56 publications

Application of erythrocyte lysing buffer (ELB) has detrimental effects on human sperm quality parameters, DNA fragmentation and chromatin structure

Application of erythrocyte lysing buffer (ELB) has detrimental effects on human sperm quality parameters, DNA fragmentation and chromatin structure

Canine and Feline Epididymal Semen—A Plentiful Source of Gametes

Sperm Selection Procedures for Optimizing the Outcome of ICSI in Patients with NOA

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