Effect of erythrosine- and LED-mediated photodynamic therapy on buccal candidiasis infection of immunosuppressed mice and Candida albicans adherence to buccal epithelial cells

Costa, Anna Carolina Borges Pereira; Rasteiro, Vanessa Maria Campos; Hashimoto, Emily Setsuko Halter da Silva; Araújo, Cássia Fernandes; Pereira, Cristiane Aparecida; Junqueira, Juliana Campos; Jorge, Antônio Olavo Cardoso

doi:10.1016/j.oooo.2012.02.002

Cited by 52 publications

(68 citation statements)

References 28 publications

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“…The power density of the LED curing unit in the present study was relatively low; thus, we decided to increase the concentration of erythrosine to obtain enough killing. There have been studies of photodynamic therapy that kills C. albicans by using erythrosine as a photosensitizer, but it was stimulated by green light, which better matches erythrosine absorption (400 μM erythrosine + green LED 42.63 J/ cm 2 led to a reduction of 0.74 log 10 [7]; 400 μM erythrosine + green LED 14.34 J/cm 2 led to a reduction of 0.73 log 10 [8] in the amount of C. albicans present in mouse lesions; and 3000 μM erythrosine + green LED 50 J/cm 2 led to a reduction of C. albicans by 4 log 10 [5]). However, in this study, we used blue light because it has been used routinely in dental clinics.…”

Section: Discussionmentioning

confidence: 99%

“…It is a photosensitizer that absorbs light at 526 nm [4]. There are studies about erythrosine as a photosensitizer for use in killing Staphylococcus aureus [5], Streptococcus mutans [6], and C. albicans [7,8] when activated by green light. Titanium dioxide is an oxide of titanium that has been widely used in industrial and consumer products [9], whose light-curing unit is blue LED wavelengths of 400-500 nm [10].…”

Section: Introductionmentioning

confidence: 99%

“…Previous studies have shown the effect of photosensitizers on bactericides and fungicides in solution form [5][6][7][8]. In clinical situations, however, it is difficult to maintain this form in the target area, e.g., deep periodontal pocket.…”

Section: Introductionmentioning

confidence: 99%

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Fungicidal effect of combined nano TiO2 with erythrosine for mediated photodynamic therapy on Candida albicans: an in vitro study

et al. 2017

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Background and objective Candida albicans is an opportunistic pathogen that can cause oral candidiasis. Antifungal drugs have been used for treatment, but resistance to these drugs has emerged in recent years. Photodynamic therapy is one of the alternative treatments. The purpose of this study was to evaluate the killing of C. albicans biofilms in photodynamic therapy in vitro by erythrosine (Ery) gel on its own or in combination with nano-titanium dioxide (TiO 2 ) stimulated by blue light (BL). Methods Four test groups were studied, namely, Ery gel at 220 and 440 μM, in the presence or absence of 1% TiO 2 + BL, and the control group. After C. albicans biofilms were prepared, a photosensitizer was applied to them for 15 min. Test groups were then activated by BL with 15 J/cm 2 energy for 1 min, and the number of CFU/mL in log 10 was compared between the test and control groups. In addition, the generation of reactive oxygen species by the effective photosensitizer was tested by electron spin resonance spectrometer (ESR) using 2,2,6,6-tetramethyl-4-piperidone and 5,5-dimethyl-1-pyrroline N-oxide. Results C. albicans survival rates were different with statistical significance across all groups by the Kruskal-Wallis test (p < 0.001). The group of 440 μM Ery gel + 1% TiO 2 + BL showed the highest efficacy (p = 0.05). Results of ESR showed that all Ery gel + BL groups generated significantly more singlet oxygen compared with control groups. None of the groups, however, generated detectable levels of hydroxyl radical and superoxide anion. Conclusion Erythrosine with blue light is an effective photosensitizer that can kill C. albicans, and nano-titanium dioxide acts as a catalyst that enhances the effect of erythrosine.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Fungicidal effect of combined nano TiO2 with erythrosine for mediated photodynamic therapy on Candida albicans: an in vitro study

et al. 2017

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“…arc, metal halide and phosphor-coated sodium lamps, are available for PDT [1][2][3]. Recently, LEDs have been applied in PDT for oral cancer and infection therapies [10,11]. The advantages of LEDs include small size, highly flexible and low cost in comparison with conventional lasers.…”

Section: Discussionmentioning

confidence: 99%

Antibacterial and cytotoxic effects of photoexcited Au clusters via blue high-power or white low-power light-emitting diode irradiation

Miyata¹,

Miyaji²,

Kawasaki³

et al. 2017

Biol Eng Med

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The development of photosensitizers and light sources has enabled the use of antimicrobial photodynamic therapy (aPDT) in various dental therapies. In the present study, we compared the antibacterial and cytotoxic effects of Au clusters photoexcited by blue and white LED irradiation. We fabricated novel photosensitizers, captopril-protected gold (Capt-Au) clusters and lysozyme-stabilized gold (Lyz-Au) clusters, for aPDT. Au clusters were then photoexcited by two kinds of light sources, blue high-power and white low-power light-emitting diodes (LEDs). Since white LED contains a wide spectrum of light (400-750 nm), white LED would be relevant for aPDT even if using a low-power source.The turbidity and viability of Streptococcus mutans were assessed following application of Capt-Au clusters (500 μg/mL) or Lyz-Au clusters (1,000 μg/mL) photoexcited by a blue high-power LED (1,000 mW/cm 2 ) or white low-power LED (80 mW/cm 2 ). In addition, the cytotoxicity of Au clusters and LED irradiation was evaluated in NIH3T3 and MC3T3-E1 cells.Au clusters photoexcited by the white low-power LED equally decreased the turbidity and viability of S. mutans compared with blue high-power LED. However, Au clusters photoexcited by white LED irradiation caused decreased cytotoxicity in mammalian cells compared with those photoexcited by blue LED irradiation. In conclusion, white LEDs possess biosafe properties for aPDT using Au clusters.

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“…Costa et al, [21] 56 immunosuppressed mice with buccal candidiasis Erythrosine (400 μmol/L) followed by exposure to a green LED (14.34 J cm(-2)). After treatment, the yeasts recovered from the mice were quantified (CFU/mL) and analyzed for the effects of PDT on their adherence to Buccal epithelial cells.…”

Section: Pdt In Oral Lesionsmentioning

confidence: 99%

Photodynamic Therapy: The Imminent Milieu For Treating Oral Lesions

Mohanty¹

2013

JCDR

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Photodynamic therapy (PDT) is used in curative and palliative treatment of head and neck squamous cell carcinoma (HN-SCC) and other oral lesions. Oral infections (such as mucosal and endodontic infections, periodontal diseases, caries, and peri-implantitis) are among the specific targets where PDT can be applied Photodynamic therapy (PDT) efficacy depends on the local dose deposited in the lesion as well as oxygen availability in the lesion. Further long-term clinical studies are necessary in establishing a more specific place of the technique in the field of dentistry.Neeta MohaNty, MD JalaluDDiN, SreeKaNth KotiNa, SaMapiKa routray, yaShWaNt iNgale InTRoduCTIonPhotodynamic therapy (PDT) is a relatively new method for treating superficial tumours of the skin and the mucosa. Prof. Hermann von Tappeiner was the person to coin the term "photodynamic", to describe oxygen-consuming chemical reactions in vivo [1]. Currently, photodynamic therapy (PDT) is a treatment modality which involves the administration of a photosensitizing compound and the accumulation of sensitizer molecules in the target cells, followed by a selective irradiation of the lesion with visible light. Drug and light are individually non-toxic and in combination, they destroy tissues. In recent times, Canada, France, Germany, Japan, The Netherlands and U.S. have approved PDT for treating select malignancies which are intraoperative and for intracavitary use. It is also used as an investigational treatment in diseases likes psoriasis vulgaris, warts, diseases of the epidermal appendages, atherosclerosis, rheumatoid arthritis and bacterial infections. EvoluTIon of PdT

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Effect of erythrosine- and LED-mediated photodynamic therapy on buccal candidiasis infection of immunosuppressed mice and Candida albicans adherence to buccal epithelial cells

Abstract: Photodynamic therapy exhibited antifungal effects against C. albicans biofilms formed in vivo and reduced the capacity of C. albicans to adhere to BECs in vitro.

Cited by 52 publications

References 28 publications

Fungicidal effect of combined nano TiO2 with erythrosine for mediated photodynamic therapy on Candida albicans: an in vitro study

Fungicidal effect of combined nano TiO2 with erythrosine for mediated photodynamic therapy on Candida albicans: an in vitro study

Antibacterial and cytotoxic effects of photoexcited Au clusters via blue high-power or white low-power light-emitting diode irradiation

Photodynamic Therapy: The Imminent Milieu For Treating Oral Lesions

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