Effect of exogenous arginine on sugarcane (Saccharum sp.) somatic embryogenesis, free polyamines and the contents of the soluble proteins and proline

Nieves, Nadina; Sagarra, Fernando; Gonzalez, R. Vega; Lezcano, Yarianne; Cid, Mariela; Blanco, M.; Castillo, R

doi:10.1007/s11240-008-9445-2

Cited by 27 publications

(34 citation statements)

References 51 publications

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“…In Saccharum sp. it was observed that arginine enhanced total soluble proteins in embryogenic cultures during embryo differentiationmaturation (Nieves et al, 2008).…”

Section: Resultsmentioning

confidence: 98%

“…2009 The comprehension of biochemical processes is essential to elucidate the general mechanisms involved in somatic embryogenesis. However, the available information on cell metabolism and morphogenesis of embryogenetic systems is still limited (Nomura & Komamine, 1995). The histodifferentiation of somatic embryos is normally associated with changes in the synthesis and mobilization of proteins, carbohydrates, and lipids.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Patterns of protein and carbohydrate accumulation during somatic embryogenesis of Acca sellowiana

Cangahuala-Inocente

Steiner

Maldonado

et al. 2009

Pesq. agropec. bras.

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-The aim of this work was to quantify the protein, starch and total sugars levels during histodifferentiation and development of somatic embryos of Acca sellowiana Berg. For histological observations, the samples were dehydrated in a battery of ethanol, embedded in historesin and stained with toluidine blue (morphology), coomassie blue (protein bodies) and periodic acid-Schiff (starch). Proteins were extracted using a buffer solution, precipitated using ethanol and quantified using the Bradford reagent. Total sugars were extracted using a methanol-chloroform-water (12:5:3) solution and quantified by a reaction with anthrone at 0.2%. Starch was extracted using a 30% perchloric acid solution and quantified by a reaction with anthrone at 0.2%. During the somatic embryogenesis' in vitro morphogenesis and differentiation processes, the total protein levels decreased and the soluble sugars levels increased during the first 30 days in culture and remained stable until the 120 th day. On the other hand, total protein levels increased according to the progression in the developmental stages of the somatic embryos. The levels of total sugars and starch increased in the heart and cotyledonary stages, and decreased in the torpedo and pre-cotyledonary stages. These compounds play a central role in the development of somatic embryos of Acca sellowiana. Index terms: total sugars, starch, pineapple guava, metabolism. Padrões de acúmulo de proteínas e carboidratos durante a embriogênese somática de Acca sellowianaResumo -O objetivo deste trabalho foi quantificar os teores de proteína, amido e açúcares totais durante a histodiferenciação e desenvolvimento dos embriões somáticos em Acca sellowiana Berg. Para as observações histológicas, as amostras foram desidratadas em uma bateria de etanol, emblocadas em historesina e coradas com azul de toluidina (morfologia), azul de coomassie (corpos proteicos) e reativo ácido periódico de Schiff (amido). As proteínas foram extraídas usando uma solução tampão, precipitadas usando etanol e quantificadas por meio do reativo de Bradford. Os açúcares totais foram extraídos usando uma solução metanol-clorofórmio-água (12:5:3) e quantificados pela reação com antrona a 0,2%. O amido foi extraído usando uma solução de ácido perclórico a 30% e quantificado pela reação com antrona a 0,2%. Durante a diferenciação e morfogênese in vitro da embriogênese somática, os teores de proteínas totais decresceram e os açúcares solúveis aumentaram durante os 30 primeiros dias em cultura e permaneceram constantes até os 120 dias. Por outro lado, os teores das proteínas totais apresentaram incremento de acordo com a progressão nos estádios de desenvolvimento dos embriões somáticos. Os teores de açúcares totais e de amido aumentaram nos estádios cordiforme e cotiledonar e diminuíram nos estádios torpedo e pré-cotiledonar. Esses compostos exercem papel central no desenvolvimento de embriões somáticos de Acca sellowiana.Termos para indexação: açúcares totais, amido, goiabeira serrana, metabolismo.

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“…In Saccharum sp. it was observed that arginine enhanced total soluble proteins in embryogenic cultures during embryo differentiationmaturation (Nieves et al, 2008).…”

Section: Resultsmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Patterns of protein and carbohydrate accumulation during somatic embryogenesis of Acca sellowiana

Cangahuala-Inocente

Steiner

Maldonado

et al. 2009

Pesq. agropec. bras.

View full text Add to dashboard Cite

show abstract

“…In sugarcane tissue culture, the addition of arginine to culture media was found to significantly induce somatic embryogenesis and to promote plant regeneration (Nieves et al, 2008). Similarly, glycine, arginine, and cysteine positively affected somatic embryogenesis and subsequent plant regeneration; 0.75 mM glycine was the most effective treatment studied (Asad et al, 2009).…”

Section: Sugarcane Regeneration Systemsmentioning

confidence: 99%

Molecular Breeding of Grasses by Transgenic Approaches for Biofuel Production

Takahashi¹,

Takamizo²

2012

Transgenic Plants - Advances and Limitations

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“…In other study, TAYLOR et al (1992) described the induction of organogenic and embryogenic callus in 18 cultivars, as well as to obtain cell cultures in suspension. Plant regeneration from embryogenic callus obtained from immature leaves was observed by DIBAX et al (2011), NIEVES et al (2008, VICKERS et al (2005) and BLANCO et al (1997) and using MS medium (MURASHIGE & SKOOG, 1962) supplemented with 2,4 dichlorophenoxyacetic acid (2,4-D) and LIU (1993), using different combinations of 2,4-D, kinetin (KIN), benzylaminopurine (BAP) and calcium pantothenate. For in vitro plant regeneration it is necessary to carry out histological analyses to determine the route of regeneration in order to discover whether the origins of the plant are in somatic embryogenesis or organogenesis, and to determine whether or not this is a reliable technique for use in the genetic transformation of sugarcane.…”

Section: Introductionmentioning

confidence: 97%

Protocol optimization and histological analysis of in vitro plant regeneration of RB92579 and RB93509 sugarcane cultivars

et al. 2012

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Effect of exogenous arginine on sugarcane (Saccharum sp.) somatic embryogenesis, free polyamines and the contents of the soluble proteins and proline

Cited by 27 publications

References 51 publications

Patterns of protein and carbohydrate accumulation during somatic embryogenesis of Acca sellowiana

Patterns of protein and carbohydrate accumulation during somatic embryogenesis of Acca sellowiana

Molecular Breeding of Grasses by Transgenic Approaches for Biofuel Production

Protocol optimization and histological analysis of in vitro plant regeneration of RB92579 and RB93509 sugarcane cultivars

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