Effect of extraction solvent on total phenol, total flavonoid content and antioxidant activities of extract plants Punica granatum, Vitis vinifera L, Ficus carica L. and Olea europea

Savitri, Evika Sandi; Holil, Kholifah; Resmisari, Ruri Siti; Syarifah, Umayatus; Munawaroh, Shaddiqah

doi:10.1063/1.5115638

Cited by 5 publications

(4 citation statements)

References 14 publications

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“…ethanol solvent can produce a higher yield percentage than n-hexane solvent. In the extraction process, a chemical mechanism like dissolves applies where polar compounds will dissolve in polar solvents and non-polar compounds will dissolve in non-polar solvents (Savitri et al, 2019). Therefore, it is possible that papaya leaf simplicia contains a higher polarsemi-polar active compound than nonpolar compounds so that more substances are attracted and the wet extract results obtained are also a higher when macerated using ethanol as the solvent rather than n-solvent -hexane.…”

Section: Resultsmentioning

confidence: 99%

Effectiveness of n-hexane and ethanol extract of papaya (Carica papaya L.) leaves as shallot pest (Spodoptera exigua Hübner) natural insecticide

Sari

Nugroho

Sukirno³

2022

JBIOUNUD

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One of the agricultural commodities that has a high selling value and consumption level is shallot (Alium ascalonicum). However, the productivity of shallots is known to be susceptible to plant-destroying organisms, such as the shallot caterpillar (Spodoptera exigua). Currently, most shallot farmers control S. exigua using synthetic insecticides. Synthetic insecticides are poisonous and use for a long time period will cause resistance to pests and environmental pollution. One of the natural ingredients that has the potential as a botanical insecticide is papaya (Carica papaya). This study aimed to determine the effectiveness and concentration of n-hexane and ethanolic extracts of papaya leaves which are the most effective against toxicity and inhibition of the feeding power of S. exigua second instar larvae. The extract was obtained by gradual maceration of n-hexane (non polar) and ethanol (polar) solvents. Test the secondary metabolite content of papaya leaves using Thin Layer Chromatography (TLC). The results showed that the ethanol extract was the most effective extract in causing toxicity to larvae with the highest percentage of 96.67%. Meanwhile, n-hexane extract was more effective in inhibiting larval feeding than ethanol extract with the lowest feeding area of 2,27 mm. The most effective concentration of against toxicity and feeding inhibition of larvae is concentration 3%. The LC50 value of the ethanol extract was 0.0207% and the n-hexane extract was 0.0459%. Both extracts are known to contain compound groups, namely tannins, terpenoids, flavonoids, and alkaloids.

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Section: Resultsmentioning

confidence: 99%

Effectiveness of n-hexane and ethanol extract of papaya (Carica papaya L.) leaves as shallot pest (Spodoptera exigua Hübner) natural insecticide

Sari

Nugroho

Sukirno³

2022

JBIOUNUD

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“…Whereas, according to the principle that like solvent like polar metabolites will be dissolved in polar solvents, while nonpolar substances in nonpolar solvents (17). Solvents such as ethanol and methanol are thoroughly employed and effective in the extraction of phenolic compounds found in natural products (18,19). Thus, in order to evaluate the effect of solvent on the extraction of phenolic compounds, the determination of total phenols and flavonoid contents was carried out.…”

Section: Resultsmentioning

confidence: 99%

Hydroalcoholic Extract of Encholirium spectabile Mart. (Bromeliaceae) in O/W Emulsions as an Additive against the UVB Radiation

Dantas

Lira

Dutra

et al. 2022

Photochem & Photobiology

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The use of sunscreen is one of the photoprotection measures most used by the population, so these products must offer broad-spectrum protection against UVA and UVB radiation. Encholirium spectabile, popularly known as "macambira-deflecha," is a species characterized by its rocky outcrops and found in the Caatinga. This biome is known for extreme droughts and dry periods, and for this reason, its vegetation needed to develop resistance mechanisms. The aim of this work was to evaluate the use of E. spectabile incorporated in O/W emulsion as a potential photoprotective agent and their antioxidant activity. Four chemical constituents (ferulic acid, caffeic acid, p-coumaric acid and apigenin) were identified by HPLC-DAD analysis, and emulsions containing different concentrations (1%, 2.5% and 5%) of the extract without and with the addition of chemical filters (octyl methoxycinnamate and benzophenone-3) were prepared and submitted to the test of preliminary stability. The Q formulation demonstrated little variation in the preliminary stability test and was selected for estimated your protection against UVB and determination of in vitro protection factor UVA. The formulations remained stable during the freeze-thaw cycle; the extract despite maintaining the UVA-PF and decreasing the wavelength showed an increase in FPS from 14.4 (control) to 18.8 (control+ Es-HA80).

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“…As is known, the antiradical activity of extract or antioxidant substances is related to their ability hydrogen-donating performance and DPPH or ABTS .+ radicals convert them a stable molecule by accepting an electron from the extract or other antioxidant substances (Gulcin et al, 2004). So, the most of the bioactive compounds generally are donors of hydrogen atoms (H) and the radicals of DPPH capture the H atoms and convert to the neutral form (Savitri et al, 2019). DPPH results were given as % inhibition of DPPH radical and tabulated in Table 2 for all solvent types.…”

Section: Results and Discussion Bioactive Compound Levels Of The Samplesmentioning

confidence: 99%

Multiple response optimization to determine the suitable solvent for the extract production from defatted grape seed powder: A simplex lattice mixture design approach

Karaman¹

2020

International Journal of Agriculture Environment and Food Sciences

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In this study, effects of different solvents (ethanol, methanol and water) on bioactive performance of defatted grape seed powder (GSP) were investigated using simplex lattice mixture design approach. Also, multiple response optimization process was applied to determine the best solvent type for the high bioactive GSP extract production. For this purpose, the bioactive compound concentrations and their antioxidant and antiradical properties were characterized and the effect of solvent type on the processing variables was modelled. Total phenolic and flavonoid contents of GSP ranged between 0.31-7.29 mg GAE/g and 44.3-537.4 mg CE/kg sample respectively. In addition to that, DPPH and ABTS .+ radical scavenging activity of the samples were in the range of 2.11-80.5% and 0.31-4.08 μg Trolox/ g sample respectively. The effect of solvent type showed a significant effect on all studied bioactive parameters and the best solvent mixture was determined as ethanol (33.84%), methanol (20.17%) and water (45.99%) by the considering the all studied parameters.

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Effect of extraction solvent on total phenol, total flavonoid content and antioxidant activities of extract plants Punica granatum, Vitis vinifera L, Ficus carica L. and Olea europea

Cited by 5 publications

References 14 publications

Effectiveness of n-hexane and ethanol extract of papaya (Carica papaya L.) leaves as shallot pest (Spodoptera exigua Hübner) natural insecticide

Effectiveness of n-hexane and ethanol extract of papaya (Carica papaya L.) leaves as shallot pest (Spodoptera exigua Hübner) natural insecticide

Hydroalcoholic Extract of Encholirium spectabile Mart. (Bromeliaceae) in O/W Emulsions as an Additive against the UVB Radiation

Multiple response optimization to determine the suitable solvent for the extract production from defatted grape seed powder: A simplex lattice mixture design approach

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