Effect of Fat Graftıng on Postoperatıve Intraabdomınal Adhesions on a Rat Model

Çil, Asudan Tuğçe Bozkurter; Aydoğdu, İlhami Oğuzhan

doi:10.1016/j.arcmed.2018.09.009

Cited by 4 publications

(4 citation statements)

References 11 publications

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“… 18 – 21 Cil and Aydogdu hypothesized that autologous fat grafting boosts remesotheliazation and decrease adhesions. 22 Formation of adhesions is a complex cascade with many factors involved, including haemostasis, inflammation, peritoneal wound healing, mesothelial regeneration and extracellular matrix production. Instead of just blocking one pathway or using mechanical contact prevention of the peritoneal surfaces, fat grafting can target many of these factors simultaneously.…”

Section: Discussionmentioning

confidence: 99%

Preperitoneal Fat Grafting Inhibits the Formation of Intra-abdominal Adhesions in Mice

Laukka

Hoppela

Salo

et al. 2020

Journal of Gastrointestinal Surgery

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Background Adhesion formation contributes to postoperative complications in abdominal and gynaecological surgery. Thus far, the prevention and treatment strategies have focused on mechanical barriers in solid and liquid form, but these methods are not in routine use. As autologous fat grafting has become popular in treatment of hypertrophic scars because of its immunomodulatory effects, we postulated that fat grafting could also prevent peritoneal adhesion through similar mechanisms. Methods This was a control versus intervention study to evaluate the effect of fat grafting in the prevention on peritoneal adhesion formation. An experimental mouse model for moderate and extensive peritoneal adhesions was used (n = 4-6 mice/group). Adhesions were induced mechanically, and a free epididymal fat graft from wild type or CAG-DsRed mice was injected preperitoneally immediately after adhesion induction. PET/CT imaging and scaling of the adhesions were performed, and samples were taken for further analysis at 7 and 30 days postoperation. Macrophage phenotyping was further performed from peritoneal lavage samples, and the expression of inflammatory cytokines and mesothelial layer recovery were analysed from peritoneal tissue samples. Results Fat grafting significantly inhibited the formation of adhesions. PET/CT results did not show prolonged inflammation in any of the groups. While the expression of anti-inflammatory and anti-fibrotic IL-10 was significantly increased in the peritoneum of the fat graft-treated group at 7 days, tissue-resident and repairing M2 macrophages could no longer be detected in the fat graft at this time point. The percentage of the continuous, healed peritoneum as shown by Keratin 8 staining was greater in the fat graft-treated group after 7 days. Conclusions Fat grafting can inhibit the formation of peritoneal adhesions in mice. Our results suggest that fat grafting promotes the peritoneal healing process in a paracrine manner thereby enabling rapid regeneration of the peritoneal mesothelial cell layer.

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Section: Discussionmentioning

confidence: 99%

Preperitoneal Fat Grafting Inhibits the Formation of Intra-abdominal Adhesions in Mice

Laukka

Hoppela

Salo

et al. 2020

Journal of Gastrointestinal Surgery

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“…There is strong evidence that fat transplantation prevents the occurrence of PAs by promoting the rapid regeneration of damaged PMCs. Autologous fat transplantation was also found in rat models of cecal wall and peritoneal injury to promote mesothelial healing and reduce PA formation in rats [ 115 ]. Autologous peritoneal grafts including PMCs can prevent PA formation by promoting the formation of a healthy peritoneum and mesothelial remodeling of the lesion [ 116 ].…”

Section: Prevention and Treatment Strategies For Pas From The Perspec...mentioning

confidence: 99%

“…The above evidence shows that both mesenchymal stem cells and autologous peritoneal grafts can effectively promote the regeneration of PMCs and play a role in preventing PA formation. Moreover, mesenchymal stem cells and autologous grafts have the advantages of easy access and low immune rejection [ 41 , 115 , 116 ]. Therefore, mesenchymal stem cells and autologous peritoneal grafts may be the most promising approaches in reducing PA formation by promoting the regeneration of PMCs.…”

Section: Prevention and Treatment Strategies For Pas From The Perspec...mentioning

confidence: 99%

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Mechanisms of Peritoneal Mesothelial Cells in Peritoneal Adhesion

Wang

Guo

2022

Biomolecules

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A peritoneal adhesion (PA) is a fibrotic tissue connecting the abdominal or visceral organs to the peritoneum. The formation of PAs can induce a variety of clinical diseases. However, there is currently no effective strategy for the prevention and treatment of PAs. Damage to peritoneal mesothelial cells (PMCs) is believed to cause PAs by promoting inflammation, fibrin deposition, and fibrosis formation. In the early stages of PA formation, PMCs undergo mesothelial–mesenchymal transition and have the ability to produce an extracellular matrix. The PMCs may transdifferentiate into myofibroblasts and accelerate the formation of PAs. Therefore, the aim of this review was to understand the mechanism of action of PMCs in PAs, and to offer a theoretical foundation for the treatment and prevention of PAs.

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