Effect of feeding methods on the astaxanthin production by Phaffia rhodozyma in fed-batch process

Abstract

The effect of feeding methods on the production of astaxanthin by the yeast Phaffia rhodozyma ATCC 24202 was studied, using continuous and pulsed fed-batch processes and low cost materials as substrates (sugar cane juice and urea). In continuous fed-batch processes, a cellular astaxanthin concentration of 383.73 µg/g biomass was obtained. But in pulsed fed-batch processes a reduction in the cellular astaxanthin concentration (303.34 µg/gbiomass) was observed. Thus the continuous fed-batch processes could be an alternative to industrial production of astaxanthin, allowing an increase in the biomass productivity without losses on astaxanthin production by the yeast.

Carotenoids from Rhodotorula and Phaffia: yeasts of biotechnological importance

“…The major carotenoid pigments produced by the yeasts Rhodotorula are -carotene, torulene (3Ј,4Ј-didehydro---carotene), and torularhodin (3Ј,4Ј-didehydro---caroten-16Ј-oic acid), in various proportions [9-11, 15, 16, 18, 21, 23, 24, 63-67] and astaxanthin (3,3Ј-dihydroxy-, -carotene-4,4Ј-dione) by PhaYa rhodozyma [20,22,28,31,[68][69][70][71][72][73][74][75][76][77]. The relative share of the three major carotenoid pigments in the total carotenoids is highly variable, and depends on diVerences between strains of the same species and is strongly inXuenced by the cultivation conditions.…”

“…Yeasts can synthesize carotenoids when cultivated in synthetic medium, containing various reWned carbon sources, such as glucose [9,22,23,25,31,[80][81][82][83][84][85][86], xylose [74], cellobiose [85], sucrose [87,88], glycerol [71] and sorbitol [85]. Studies on carotenogenesis have led to a growing interest in using natural substrates as carbon sources: grape juice [72,89]; grape must [11,63]; peat extract and peat hydrolysate [20,21,77,90]; date juice of Yucca Wllifera [76]; hydrolyzed mustard waste isolates [28]; hemicellulosic hydrolysates of eucalyptus globules wood [68,75]; hydrolyzed mung bean waste Xour [91]; sugar cane juice [69,73,78]; sugar cane and sugar-beet molasses [8,10,18,92,93]; corn syrup [18,64]; corn hydrolysate [94,95]; milk whey [15,16,…”

Rhodosporidium sp. GROWTH IN MOLASSES MEDIUM AND EXTRACTION OF ITS ASTAXANTHIN BY USING HCl

“…1.3 times as lower as wild yeast Phaffia rhodozyma ATTCC2402 was fermented to produce 303.3 μg/g of astaxanthin content [18] (Figures 13 and 14, Table 2). In the experiment of Tong and Tran [19], yeast Phaffia rhodozyma NT5 was grown in liquid culture containing saccharose 20 g/l at pH 5.0 at 22 o C in 120 hours, and produced 285.4 μg/g of astaxanthin content, inconsiderably higher than the content taken from Rhodosporidium sp.…”

Astaxanthin production from sewage of traditional Thai rice vermicelli

“…Many researchers tried to optimize astaxanthin biosynthesis by pH control strategy [15,22], fed-batch strategy [23,25,27], light type and intensity [24], nitrogen source [21] and citrate supplement [26]. Figure 3 shows the experimental results from batch culturing of Phaffia rhodozyma TISTR 5730 in coconut water (CN) adjusted citrate concentration to 0.5, 1.0 and 1.5 g/L (fixed glucose concentrations at 15 g/L by adding fan palm sugar).…”