Effect of five dietary antimutagens on the genotoxicity of six mutagens in the microscreen prophage-induction assay

Cabrera, Guillermo

doi:10.1002/1098-2280(2000)36:3<206::aid-em4>3.0.co;2-u

Cited by 15 publications

(4 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Ascorbic acid (AA) is involved in various biological processes, including free radical scavenging, and it has been demonstrated that treatment with AA significantly reduced the genotoxicity of well-known mutagens (Odin, 1997;Cabrera, 2000;Nefic, 2001;Rao et al, 2001;Kaya et al, 2002;Siddique et al, 2005). However, administration of large amounts of AA to biological systems has lead to genotoxic effects in several different test systems (Shamberger, 1984) because AA has both antioxidant and prooxidant activities (Bijur et al, 1997).…”

Section: Introductionmentioning

confidence: 99%

Modulatory effects of the antioxidant ascorbic acid on the direct genotoxicity of doxorubicin in somatic cells of Drosophila melanogaster

2007

View full text Add to dashboard Cite

In this study two different crosses involving the wing cell markers mwh and flr 3 (standard (ST) cross and high bioactivation (HB) cross, the latter being characterized by a high constitutive level of cytochrome P450 which leads to an increased sensitivity to a number of promutagens and procarcinogens) were used to investigate the modulatory effects of ascorbic acid (AA) combined with the antitumor agent doxorubicin (DXR) in Drosophila melanogaster. We observed that the two different concentrations of AA (50 or 100 mM) had no effect on spots frequencies, while DXR treatments (0.2 or 0.4 mM) gave positive results for all types of spots, when compared to negative control. For marker-heterozygous (MH) flies, a protective effect was observed with the lower concentration of AA (50 mM) that was able to statistically decrease the frequency of spots induced by DXR (0.2 mM), while an enhanced frequency of spots induced by DXR was observed with the higher concentration of AA (100 mM), when compared to DXR treatment (p < 0.05). These results suggest that AA may interfere with free radicals generated by DXR and with other possible reactive metabolites. The efficiency of AA in protecting the somatic cells of D. melanogaster against mutation and recombination induced by DXR is dependent on the dose used and the protection is directly related to the activity of cytochrome P450 enzymes.

show abstract

Section: Introductionmentioning

confidence: 99%

Modulatory effects of the antioxidant ascorbic acid on the direct genotoxicity of doxorubicin in somatic cells of Drosophila melanogaster

2007

View full text Add to dashboard Cite

show abstract

“…However, in the E. coli assay with metabolic activation, which is known to be sensitive to PCP genotoxicity, the two base fractions were the most potent although they contained some of the lowest PCP levels. The E. coli assay detects BP genotoxicity as well (21). The two base fractions contained among the lowest BP concentrations of the crude extracts and fractions, however, suggesting that the BP in these fractions did not fully account for the strong response induced by these fractions in the E. coli assay.…”

Section: Discussionmentioning

confidence: 96%

Toxicity Characterization of Complex Mixtures Using Biological and Chemical Analysis in Preparation for Assessment of Mixture Similarity

Cizmas

McDonald

Phillips

et al. 2004

Environ. Sci. Technol.

View full text Add to dashboard Cite

In the United States, several proposed approaches for using bioassays for the risk assessment of complex hazardous mixtures require that selected mixtures be "sufficiently similar" to each other. The goal of this research was to evaluate the utility of a protocol using in vitro bioassays and chemical analysis as a basis for assessing mixture similarity. Two wood preserving wastes (WPWs) containing polycyclic aromatic hydrocarbons and pentachlorophenol were extracted and fractionated to generate potentially similar mixtures. Chemical analysis was conducted using gas chromatography/mass spectrometry. Genotoxicity was evaluated using the Salmonella/microsome and Escherichia coli prophage induction assays. The crude extract of one WPW was also tested in the chick embryotoxicity screening test (CHEST) assay. The CHEST assay provided the most sensitive measurement of toxicity. Overall, the biological potency of the samples was not well correlated with predicted potency based on chemical analysis. Although several mixtures appeared similar based on chemical analysis, the magnitude of the response in bioassays was often dissimilar. Fractionation was required to detect the genotoxicity of mixture components in vitro. The results confirm the need for an integrated protocol, combining chemical analysis, fractionation, and biological testing to characterize the risk associated with complex mixtures.

show abstract

“…Some of these protection studies have shown beneficial effects of these extracts against DXR toxicity (CABRERA, 2000;QUILES et al, 2002). Doxorubicin (DXR) is an antineoplastic drug which is cell cycle specific for the S phase of cell division (KUSYK & HSU, 1976).…”

Section: Discussionmentioning

confidence: 99%

<htm>Supressão de doxorubina opoptótica, histopatológica, mutagênica e efeito de estresse oxidativo em medula óssea e tecido testicular de ratos por extrato aquoso de folhas de alecrim</htm>

Abdella¹,

Ahmed²

2009

ABPar

View full text Add to dashboard Cite

show abstract

Effect of five dietary antimutagens on the genotoxicity of six mutagens in the microscreen prophage-induction assay

Cited by 15 publications

References 45 publications

Modulatory effects of the antioxidant ascorbic acid on the direct genotoxicity of doxorubicin in somatic cells of Drosophila melanogaster

Modulatory effects of the antioxidant ascorbic acid on the direct genotoxicity of doxorubicin in somatic cells of Drosophila melanogaster

Toxicity Characterization of Complex Mixtures Using Biological and Chemical Analysis in Preparation for Assessment of Mixture Similarity

<htm>Supressão de doxorubina opoptótica, histopatológica, mutagênica e efeito de estresse oxidativo em medula óssea e tecido testicular de ratos por extrato aquoso de folhas de alecrim</htm>

Contact Info

Product

Resources

About