Effect of Flaxseed Extract on the Liver Histological Structure in Streptozotocin Induced Diabetic Rats

Abstract

Introduction: Diabetes mellitus has become a serious warning to mankind health all over the world. The management goal of diabetes is to keep blood glucose levels as close as possible to healthy individuals. Medications used to treat diabetes are usually associated with complications and may cause different side effects. Many traditional anti-diabetic plants have become popular in the management of diabetes mellitus. Flaxseed has been used as traditional medicine for centuries. Objective: This study aimed to evaluate the hepatoprotective effects of flaxseed extract in streptozotocin (STZ)-induced diabetic rats. Methods: Diabetes mellitus was induced in Sprague-Dawley rats using a single injection of streptozotocin (60 mg/kg i.p.). The rats were divided into five groups of 8 rats each. Group NC, normal control rats; Group NF, normal rats treated with flaxseed extract (400 mg/kg); Group DC, diabetic control rats; Group DG, diabetic rats treated with glibenclamide (0.6 mg/kg); Group DF, diabetic rats treated with flaxseed extract (400 mg/kg); for 4 weeks. Results: There were significant increase in relative liver weight, blood glucose levels in DC group comparing to NC group (p<0.05). The disturbance of these parameters was ameliorated in DF and DG groups. Histological observation revealed congestion of central veins, degeneration of hepatocytes, and reduced glycogen granules in DC group. These pathological changes were ameliorated in the flaxseed extract and glibenclamide treated rats. Conclusion: Flaxseed extract may represent a candidate alternative treatment to control diabetes mellitus and its related hepatopathy.

Human Umbilical Cord Mesenchymal Stem Cell-Derived Small Extracellular Vesicles Ameliorated Insulin Resistance in Type 2 Diabetes Mellitus Rats

“…The treatment groups of 0.25 and 1 mg/kg hUC-MSCs-sEVs and glibenclamide had restored normal liver architecture with no specific pathology or inflammation compared to untreated diabetic rats. This finding agrees with a previous study by Al-ani et al [ 26 ], who found glibenclamide-treated STZ-induced diabetic rats showed fewer pathological changes to liver architecture, indicating a protective effect of the treatment against hepatic changes associated with diabetes. In addition, the finding is supported by Diaz-Juarez et al [ 27 ], who found that elevated hepatic enzymes did not correlate with cell necrosis.…”

Histopathology of Liver and Cardiac Muscle of Alloxan-Induced Diabetic Rats (Rattus norvegicus) After Administration of Bosibosi (Timonius flavescens (Jacq.) Baker) Leaves Ethanol Extract

“…The liver plays a pivotal role in glucose and lipid homeostasis, is severely affected by diabetes [34]. Al-Ani et al [35] reported the presence of morphological and histological alterations in liver tissues of rats treated with alloxan, as indicated by the increase in liver weight, glycogen reduction, associated with lipid deposition, inflammatory cells infiltration and Kupffer cells hyperplasia. It has been previously reported that alloxan causes central venous congestion with significant dilatation of the sinusoidal spaces of liver tissue [36].…”