Effect of four probiotic strains and Escherichia coli O157:H7 on tight junction integrity and cyclo-oxygenase expression

Putaala, Heli; Salusjärvi, Tuomas; Nordström, Malin; Saarinen, Markku; Ouwehand, Arthur C.; Hansen, Egon Bech; Rautonen, Nina

doi:10.1016/j.resmic.2008.08.002

Cited by 95 publications

(72 citation statements)

References 39 publications

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“…As an inducible enzyme, COX-2 has been implicated in proinflammatory signalling, and its transcription is up-regulated, for instance, during stimulation with pathogenic enterohemorrhagic E. coli (Putaala et al, 2008). Thymol and cinnamaldehyde did not alter COX2 transcript levels in our study to a large extend.…”

Section: Discussioncontrasting

confidence: 43%

“…Caco-2 cells (66 000 cells/cm 2 , ATCC, P35) were differentiated as described in Putaala et al (2008). Shortly, the cells were seeded in 12-well fibrillar collagen-coated cell culture inserts (Corning, New York, NY, USA) in Caco-2 maintenance medium without antibiotics in both apical and basolateral compartment and incubated overnight at 37 °C in a 5% CO 2 atmosphere with 95% humidity (Heracell 150i, Thermo Fisher Scientific, Waltham, MA, USA).…”

Section: Transepithelial Electrical Resistance (Teer) In Differentiatmentioning

confidence: 99%

“…Total RNA was isolated using the NucleoSpin RNA kit (Macherey-Nagel GmbH & Co., Duren, Germany), and reverse transcription was performed using Superscript III (Thermo Fisher Scientific, Waltham, MA, USA) with random primers according to manufacturers' instructions. The absolute levels of COX1 and COX2 were determined as described by Putaala et al (2008) with the use of thermocycler ABI PRISM ® 7000 (Applied Biosystems, Foster City, CA, USA). The COX1:COX2 ratio was calculated as the amount of COX1 mRNA divided by that of COX2 mRNA.…”

Section: Real-time Rt-pcr Analysis Of Cox1 and Cox2 Genes In Differenmentioning

confidence: 99%

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Effects of cinnamaldehyde and thymol on cytotoxicity, tight junction barrier resistance, and cyclooxygenase-1 and -2 expression in Caco-2 cells

Putaala¹,

Nurminen²,

Tiihonen³

2017

J. Anim. Feed Sci.

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Section: Discussioncontrasting

confidence: 43%

Section: Transepithelial Electrical Resistance (Teer) In Differentiatmentioning

confidence: 99%

Section: Real-time Rt-pcr Analysis Of Cox1 and Cox2 Genes In Differenmentioning

confidence: 99%

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Effects of cinnamaldehyde and thymol on cytotoxicity, tight junction barrier resistance, and cyclooxygenase-1 and -2 expression in Caco-2 cells

Putaala¹,

Nurminen²,

Tiihonen³

2017

J. Anim. Feed Sci.

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“…This appears to be a multi-factorial process involving both induction of mucus secretion from goblet cells (Mack et al, 1999;Caballero-Franco et al, 2007) and maintenance of the tight cell junctions between cells (Madsen et al, 2001;Roselli et al, 2007;Putaala et al, 2008). This function may be most important in counteracting the effects of pathogens, which often exert gastrointestinal effects by weakening the junctions between cells allowing for translocation of the pathogens and activation of inflammatory signals or establishment of local inflammatory lesions.…”

Section: Probiotic Actions On Host Physiologymentioning

confidence: 99%

Probiotics – do they have a role in the pig industry?

Kenny

Smidt

Mengheri

et al. 2011

Animal

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The delivery of certain living microorganisms in food has long been suggested as having positive health effects in humans. This practice has extended into food animal production, with a variety of microorganisms being used; lactic acid bacteria, variousBacillusspecies and the yeastSaccharomyces cerevisiaehave been particularly used in the pig industry. The increased interest in probiotics is essentially due to the problem of microbial resistance to antibiotics and following the ban of the use of antibiotics in animal production, probiotics being considered an alternative means to reduce pathogen infection and improve animal health especially around the time of weaning. However, there is still a need to clarify the probiotic effectiveness in pigs, and the underlying mechanisms. When assessing the efficacy of probiotics one must consider the particular strain of organism being used and the production stage of the pigs being treated. The reproducible delivery of probiotics in industrial pig production is problematic as maintenance of viability is key to their beneficial activity, but difficult to achieve with commonly used feed processing technologies. One specific context where probiotics organisms may be reliably delivered is in systems utilising fermented liquid feeds. Liquid feed may be fermented by the activity of wild lactic acid bacteria or may be stimulated using specific isolates as ‘starters’; the latter system has advantages in terms of reproducibility and speed of fermentation. The farm context in which the organism is used is likely to be critical; the use of probiotics is more likely to result in measurable economic gains in animals living in sub-optimal conditions rather than in those reared in the highest welfare and husbandry conditions. The establishment of a beneficial lactic acid bacteria population at birth may lead to healthier animals, this may be most effectively achieved by treating sows, which provide an amplification step and flood the neonatal pigs’ environment with desirable bacterial strains. In contrast, it may be sufficient to provide a supportive, protective microbiota around the time of weaning as this is a time of major crisis with instability and loss of certain bacterial populations.

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“…It is found that E. coli O157:H7 significantly increased the permeability of Caco-2 cells and released elements, while B. Lactis significantly reduced it [38]. Lactobacillus rhamnosus GG prevented cytokineinduced apoptosis in intestinal epithelial cell model by inhibiting activation of proapoptotic р38/ mutagen activating protein kinase [19].…”

mentioning

confidence: 99%

Therapeutic Potential of Modern Probiotics

Урсова¹

2013

Pediatr. farmakol.

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Effect of four probiotic strains and Escherichia coli O157:H7 on tight junction integrity and cyclo-oxygenase expression

Cited by 95 publications

References 39 publications

Effects of cinnamaldehyde and thymol on cytotoxicity, tight junction barrier resistance, and cyclooxygenase-1 and -2 expression in Caco-2 cells

Effects of cinnamaldehyde and thymol on cytotoxicity, tight junction barrier resistance, and cyclooxygenase-1 and -2 expression in Caco-2 cells

Probiotics – do they have a role in the pig industry?

Therapeutic Potential of Modern Probiotics

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