Effect of freezing methods on the properties of lyophilized porous silk fibroin membranes

Weska, Raquel Farias; Vieira, Wellington Carlos; Nogueira, Grínia M.; Beppu, Marisa Masumi

doi:10.1590/s1516-14392009000200020

Cited by 33 publications

(16 citation statements)

References 22 publications

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“…SS powder obtained was dissolved in distilled water at concentration of 2% (w/v) using an autoclave at the same conditions mentioned above. The solution obtained was poured into plates with 2-mL wells, which were frozen at − 80 °C for 24 h [28][29][30]. Then, the frozen samples were lyophilized for 48 h in a Labconco freeze dryer (USA).…”

Section: Extraction Of Ss and Preparation Of Ss Spongesmentioning

confidence: 99%

Influence of ethanol post-treatments on the properties of silk protein materials

et al. 2019

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The use of silk proteins sericin (SS) and fibroin (SF) has been increased for biomedical applications. In order to improve their behavior inside of biological environment, silk biomaterials must be treated after their manufacturing by means of diverse methods. These include ethanol post-treatments to increase their crystallinity, mechanical properties and water stability. In this study, the effect of ethanol post-treatments on the properties of silk protein materials was evaluated. Defective cocoons and silk fibrous waste (SW) were used to obtain silk sericin sponges (S-SS) and silk fibroin films (F-SF), respectively. Two ethanol treatments were evaluated in SS and SF: immersion (I) and solvent vapor annealing (SVA). Morphological modifications induced by ethanol post-treatments were studied by scanning electron microscopy (SEM). Conformational structure of the samples was analyzed by attenuated total reflectance-Fourier-transform infrared spectroscopy (ATR-FTIR), and the thermal properties were evaluated by differential scanning calorimetry (DSC) measurements. SEM images revealed that ethanol process induces changes in treated F-SF and S-SS, the material surfaces are more roughness, and these effects were more pronounced in samples treated by I than that subjected to SVA. As a result of the ethanol treatments, the ATR-FTIR and DSC results showed an increment in relative content of β-sheet structures in both silk protein materials. The results suggest that ethanol post-treatments induce conformational transitions and morphological modifications in S-SS and F-SF that should be considered to select the post-treatment conditions according to the biomedical application requirements.

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Section: Extraction Of Ss and Preparation Of Ss Spongesmentioning

confidence: 99%

Influence of ethanol post-treatments on the properties of silk protein materials

et al. 2019

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“…For the pore size measurements (Figure 3), it is important to note that the hydrogels were lyophilized for SEM imaging after crosslinking. Lyophilization, or freeze-drying, is a process that has been utilized to promote formation of porous networks due to ice crystallization during freezing [51,52,53,54,55]. Thus, the actual pore sizes of the hydrated hydrogel may be significantly different than those observed under SEM.…”

Section: Discussionmentioning

confidence: 99%

Cost-Effective Cosmetic-Grade Hyaluronan Hydrogels for ReNcell VM Human Neural Stem Cell Culture

Suh

2019

Biomolecules

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Hyaluronic acid (HA) is a polysaccharide polymer frequently used as a starting material to fabricate hydrogels, especially for recapitulating the brain’s extracellular matrix (ECM) for in vitro neural stem cell (NSC) cultures. Here, we report the successful synthesis of a methacrylated HA (MeHA) polymer from an inexpensive cosmetic-grade hyaluronan starting material. The MeHA polymers synthesized from cosmetic-grade HA yielded similar chemical purity to those from pharmaceutical/research-grade HA reported in the literature. Crosslinked MeHA (x-MeHA) hydrogels were formed using radical polymerization which resulted in mechanical properties matching previously reported mechanical property ranges for enhanced neuronal differentiation of NSCs. We assessed cellular adhesion, spreading, proliferation, and stiffness-dependent neuronal differentiation properties of ReNcell VM human neural stem cells (hNSCs) and compared our results to studies reported in the literature (that utilized non-human and human pluripotent cell-derived NSCs).

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“…La FS es un polímero natural que presenta una serie de características de gran interés para el desarrollo de nuevos materiales, entre las que se destacan sus propiedades mecánicas y su biodegradabilidad [48,49]. Estas características hacen que la FS pueda tener potencial aplicación como biomaterial de empaques.…”

Section: Fibroína De Seda Como Biomaterialsunclassified

Revisión: Fibroína de seda y sus potenciales aplicaciones en empaques biodegradables para alimentos/Review: silk fibroin and their potential applications on biodegradable food packaging

et al. 2017

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RESUMENEl cuidado del medioambiente y el uso responsable de los recursos, ha promovido investigaciones con diferentes biopolímeros que permitan el desarrollo de nuevos materiales que puedan ser empleados en empaques para alimentos. Entre estos biopolímeros se encuentra la fibroína de seda (FS), que representa cerca del 70% de la fibra de seda y puede ser obtenida a partir de capullos de primera calidad o de los desperdicios generados en la producción serícola. En el desarrollo de empaques de alimentos con FS, se pueden dar dos alternativas de producción, una en la que la FS puede ser funcionalizada por otros componentes, y otra en la que se incluye la FS como componente funcional modificando otros biopolímeros. La FS puede conferirle al material de empaque ciertas propiedades, como: aumento de la permeabilidad de oxígeno, resistencia a la rotura y una mayor velocidad de degradación del biopolímero. Este artículo se centra en cuatro temas principales: empaques, biopolímeros en empaques, FS y finalmente, la FS en empaques para alimentos.Palabras clave: Empaques, Materiales poliméricos, Biopolímeros, Fibroína de seda, Biomateriales. ABSTRACTEnvironmental protection and responsible use of resources, have promoted research of different biopolymers allowing to develop biodegradable materials which can be used in food packaging. Among these biopolymers is silk fibroin (SF), this protein represents nearly 70% of the silk fiber and it can be obtained from high quality cocoons or from waste generated in the silk industry. In the development of food packaging with SF, there are two possible alternative of production, one in what the SF can be functionalized by other components and another in which, the FS is included as functional component modifying other biopolymers. In the case of food packaging, the SF can concede desirable properties to the package such as an increase in oxygen permeability, more tensile strength and a higher biopolymer degradation rate. This paper is focused on four main topics: packaging, biopolymers in packaging, SF and finally, the SF in food packaging.

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Effect of freezing methods on the properties of lyophilized porous silk fibroin membranes

Cited by 33 publications

References 22 publications

Influence of ethanol post-treatments on the properties of silk protein materials

Influence of ethanol post-treatments on the properties of silk protein materials

Cost-Effective Cosmetic-Grade Hyaluronan Hydrogels for ReNcell VM Human Neural Stem Cell Culture

Revisión: Fibroína de seda y sus potenciales aplicaciones en empaques biodegradables para alimentos/Review: silk fibroin and their potential applications on biodegradable food packaging

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