Effect of freezing temperature in thermally induced phase separation method in hydroxyapatite/chitosan-based bone scaffold biomaterial

The influence of phosphorylation and freezing temperature on the mechanical properties of hydroxyapatite/chitosan composite as bone scaffold biomaterial

“…However, upon the completion of this research, it has been obtained the result which is already improved from the previous research. In our previous work [5], at -20 o C the achieved compressive strength was 5.9 KPa without phosphorylation. In the current investigation, at the same freezing temperature with phosphorylated chitosan, it was measured as 38.4 KPa, which is increased dramatically up to ~550%.…”

“…This material does not cause any negative response from the body because it is one of the constituents of human bone. There have been many studies conducted to fabricate a bone scaffold based on hydroxyapatite/chitosan to obtain appropriate pore size [5][6][7][8][9]. Previously, hydroxyapatite/chitosan bone scaffold has been produced with the pore size of 100-300 μm using thermally induced phase separation (TIPS) method [5].…”

“…There have been many studies conducted to fabricate a bone scaffold based on hydroxyapatite/chitosan to obtain appropriate pore size [5][6][7][8][9]. Previously, hydroxyapatite/chitosan bone scaffold has been produced with the pore size of 100-300 μm using thermally induced phase separation (TIPS) method [5]. However, its compressive strength was reported to be lower than the biomechanical properties of healthy bone [10].…”