Effect of fruit extract of Fragaria vesca L. on experimentally induced inflammatory bowel disease in albino rats

Abstract

Aim:Ulcerative colitis and Crohn’s disease are chronic recurrent inflammatory bowel disease (IBD) of unknown origin. Oxidative stress is believed to be a key factor in the pathogenesis and perpetuation of the mucosal damage in IBD.Materials and Methods:Ethanolic extract of Fragaria vesca (EFFV) fruits was prepared by percolation method and subjected to oral toxicity testing using OECD guidelines. Albino rats were pretreated orally for 5 days with 3% gum acacia in control, EFFV 500 mg/kg in test and 5-aminosalisylic acid (5-ASA) 100 mg/kg in standard groups. Colitis was induced by transrectal administration of 4% acetic acid on 5th day. All the animals were sacrificed with ether overdose 48 hours after colitis induction, and 10 cm colon segment was resected from proximal end. Colon was weighed (for disease activity index) and scored macroscopically and microscopically after histological staining. Biochemical assessments included myeloperoxidase (MPO) and tissue catalase (CAT), glutathione (GSH) and superoxide dismutase (SOD) measurements. Statistical analysis was done using one-way analysis of variance (ANOVA) followed by Dunnett’s “t” test.Results:EFFV showed significant (P < 0.05) prevention of increase in colon weight and disease activity index along with decrease in macroscopic and microscopic lesion score as compared to control group. Significant improvement was observed in the levels of MPO, CAT and SOD, except GSH (P < 0.05). However, the effect of EFFV was significantly less than 5-ASA (P < 0.05).Conclusions:EFFV at 500 mg/kg showed significant amelioration of experimentally induced IBD, which may be attributed to its antioxidant and anti-inflammatory properties.

Triphala, an Ayurvedic formulation improves the antioxidant status on TNBS induced IBD in rats

“…In support of these observations and the role of ROS generation in TNBS colitis, Loguercio et al [29] reported that glutathione supplementation decreased oxidative damage in TNBS colitis. Similarly, Kanodia et al [30] recorded that fruit extract of Fragaria vesca exerts significant reduction in the levels of myeloperoxidase with concomitant increase in CAT and SOD in rat IBD models. Role of three different forms of Nitric Oxide Synthases (NOS), namely eNOS, iNOS and nNOS has been studied in TNBS and DDS induced colitis model [31].…”

To Study the Ulceroprotective Effect of Leaves of Moringa Oleifera on Experimentally Induced Inflammatory Bowel Disease on Animal Models

“…Oxidative stress is believed to play a key role in the pathogenesis of IBD-related intestinal damage. As a matter of fact, intestinal mucosal damage in the IBD, including CD and UC, is related to both increased free radical production and to a low concentration of endogenous antioxidant defense [19].…”

Evaluation of anti-inflammatory effect of anti-platelet agent-clopidogrel in experimentally induced inflammatory bowel disease

“…One unit of MPO activity was defined as that degrading one micromole of peroxidise per minute at 25°C. This calculation was done by using following formula:[17]…”