Aim:Ulcerative colitis and Crohn’s disease are chronic recurrent inflammatory bowel disease (IBD) of unknown origin. Oxidative stress is believed to be a key factor in the pathogenesis and perpetuation of the mucosal damage in IBD.Materials and Methods:Ethanolic extract of Fragaria vesca (EFFV) fruits was prepared by percolation method and subjected to oral toxicity testing using OECD guidelines. Albino rats were pretreated orally for 5 days with 3% gum acacia in control, EFFV 500 mg/kg in test and 5-aminosalisylic acid (5-ASA) 100 mg/kg in standard groups. Colitis was induced by transrectal administration of 4% acetic acid on 5th day. All the animals were sacrificed with ether overdose 48 hours after colitis induction, and 10 cm colon segment was resected from proximal end. Colon was weighed (for disease activity index) and scored macroscopically and microscopically after histological staining. Biochemical assessments included myeloperoxidase (MPO) and tissue catalase (CAT), glutathione (GSH) and superoxide dismutase (SOD) measurements. Statistical analysis was done using one-way analysis of variance (ANOVA) followed by Dunnett’s “t” test.Results:EFFV showed significant (P < 0.05) prevention of increase in colon weight and disease activity index along with decrease in macroscopic and microscopic lesion score as compared to control group. Significant improvement was observed in the levels of MPO, CAT and SOD, except GSH (P < 0.05). However, the effect of EFFV was significantly less than 5-ASA (P < 0.05).Conclusions:EFFV at 500 mg/kg showed significant amelioration of experimentally induced IBD, which may be attributed to its antioxidant and anti-inflammatory properties.

The aim of the study was to compare the analgesic activities of ethanolic extract of fruits and whole plant of Fragaria vesca in experimental animal models. The extracts were prepared by percolation method and oral toxicity testing was performed as per OECD guidelines. Analgesic activity was assessed by tail flick method (for central action) and acetic acid-induced writhing test (for peripheral action). Fruit extract, whole plant extract and aspirin showed significant analgesic activity, both central and peripheral, as compared to control (p<0.01). Although fruit extract at dose of 500 mg/kg showed better activity than 250 mg/kg (p<0.05). Analgesic activities of fruit extract 250 mg/kg and whole plant extract 500 mg/kg were almost equivalent while aspirin was most potent among all with significantly greater activities as compared to all the extracts (p<0.05).

Background: Fragaria vesca has been used in the past for the treatment of rheumatism and gout. Material and Methods: Ethanolic extracts of fruits and the whole plant of F. vesca were prepared by the percolation method and oral toxicity testing was performed as per Organization for Economic Co-operation and Development (OECD) guidelines. The antiinfl ammatory activity against acute infl ammation was checked by the carrageenan-induced rat paw edema test, the granuloma pouch method for the subacute infl ammation, and Freund's adjuvant-induced arthritis model for chronic infl ammation in albino rats. The fruit extract was used at a dose of 500 mg/kg and the whole plant extract was also used at 500 mg/kg. Aspirin (100 mg/kg) was taken as standard. Results: The fruit extract, whole plant extract, and aspirin, all showed signifi cant anti-infl ammatory activities against acute,subacute, and chronic infl ammation as compared to the control (P < 0.01) although the fruit extract showed a better activity than the whole plant extract (P < 0.05). Conclusion: F. vesca showed a signifi cant activity against acute, sub acute, and chronic infl ammation. Anti-infl ammatory activities of all the drugs in a decreasing order were as follows: aspirin > fruit extract, 500 mg/kg > whole plant extract, 500 mg/kg.

Objective:To study the analgesic and acute anti-inflammatory effect of the ethanolic extract of the leaves of Paederia foetida (EEPF) on experimental animal models. Materials and Methods: Fresh leaves were collected, air-dried, powdered, and percolated in 95% ethanol. Acute toxicity test was done according to OECD guidelines. Healthy albino rats (150-200 gm) of either sex were taken and divided into five groups with six animals in each group for central analgesic activity by tail flick method. Peripheral analgesic activity by 1% glacial acetic acid induced writhing test by taking albino mice (20-30 gm) of either sex which were divided into three groups with six animals in each. EEPF was used in a dose of 500 mg/kg bodyweight subcutaneously for tail-flick method and orally for writhing test. In tail-flick method the basal reaction time was taken and a cut-off period of 10 sec was observed. Reaction time were recorded at predrug, 15, 30, 60, 90, 120, 150 and 180 minutes after administration of drug . The standard drug used for central analgesic activity was pethidine 5 mg/kg bw, naloxone 1mg/kg as antagonist and naloxone 1mg/kg bw with 500mg/kg bw of EEPF to study the central mechanism of action. The standard drug used for peripheral analgesic activity was aspirin 100mg/kg bw orally. A control group was maintained in all the models. For anti-inflammatory study, three groups of animals of either sex (n = 6), weighing 150-200g of the species Rattus norvegicus were taken for the study. Group A was taken as control (Normal salie, 10 mL/kg body weight), Group B as test group (EEPF 500 mg/kg body weight), and Group C as standard (Aspirin 100 mg/kg body weight). The animals were studied for acute inflammation by Carrageenan-induced rat paw edema. Statistical analysis  was done by one-way analysis of variance followed by multiple comparison tests.Results: EEPF significantly increased the reaction time in tail-flick method (p<0.05) whereas the combination of naloxone and EEPF decreased the reaction time indicating that naloxone inhibits the analgesic effect of EEPF. In 1% glacial acetic acid induced writhing EEPF reduced writhing significantly In acute inflammation, there was significant inhibition of paw edema in Groups B, C in comparison with Group A (P < 0.05). Conclusion:The ethanolic extract of Paederia foetida has significant analgesic and anti-inflammatory activity. DOI: http://dx.doi.org/10.3329/bjms.v11i3.11723 Bangladesh Journal of Medical Science Vol. 11 No. 03 July’12

Objectives:To evaluate the effect of ethanolic extract of leaves of Paederia foetida on acetic acid induced colitis in albino rats.Materials and Methods:Ethanolic extract of Paederia foetida (EEPF) was prepared by percolation method. Acute toxicity test was done by using Organization for Economic Cooperation and Development guidelines. Albino rats were divided into four groups of five animals each. Groups A and B received 3% gum acacia. Groups C and D received EEPF 500 mg/kg body weight (BW) and 5-aminosalisylic acid 100 mg/kg BW respectively. Colitis was induced by transrectal administration of 4% acetic acid on 5th day. All animals were sacrificed after 48 h of colitis induction and distal 10 cm of the colon was dissected. Colon was weighed for disease activity index (DAI) and scored macroscopically and microscopically. Biochemical assessment of tissue myeloperoxidase (MPO), catalase (CAT) and superoxide dismutase (SOD) was done in colonic tissue homogenate and malondialdehyde (MDA) was estimated in serum.Results:P. foetida showed significant (P < 0.05) reduction in DAI, macroscopic and microscopic lesion score as well as significant (P < 0.05) improvement in MPO, MDA, CAT, and SOD level as compared to Group B.Conclusions:The ethanolic extract of leaves of P. foetida showed significant amelioration of experimentally induced colitis, which may be attributed to its anti-inflammatory and antioxidant property.