Effect of furfural, acetic acid and 5-hydroxymethylfurfural on yeast growth and xylitol fermentation using Pichia stipitis NCIM 3497

Bhavana, B K; Mudliar, Sandeep N.; Bokade, Vijay V.; Debnath, Sukumar

doi:10.1007/s13399-022-02758-w

Cited by 14 publications

(5 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Indeed, xylose assimilation seems to be not an easy task for yeast cells. Pichia stipitis cells consumed around 66% of xylose after 72 h of fermentation [41] and M. caribbica consumed all xylose only after 120 h of cultivation [42], despite the experiments used in rich medium. On the other hand, Veras et al [43] reported high ethanol yields with full xylose consumption by Scheffersomyces stipitis and Spathaspora passalidarum in mineral medium under oxygen-limited conditions.…”

Section: Discussionmentioning

confidence: 95%

“…This organic acid can induce intracellular acidification that increases the demand for ATP and impairs the metabolic flux through the central metabolism [47]. The xylitol production by P. stipitis NCIM 3497 was also inhibited in the presence of 3.7 g/L of acetic acid [41]. Tadioto et al [22] also reported the impairment of xylose consumption and xylitol production by M. caribbica from stalk-straw corn hydrolysates that contained acetic acid above 3 g/L.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Meyerozyma Caribbica Isolated From Vinasse-Irrigated Sugarcane Plantation Soil: A Promising Yeast for Ethanol and Xylitol Production in Biorefineries

Alencar¹,

Freitas²,

Silva³

et al. 2023

Preprint

View full text Add to dashboard Cite

The production of fuels and other industrial products from renewable sources has intensified the search for new substrates or for the expansion of the use of substrates already in use, as well as the search for microorganisms with different metabolic capacities. In the present work, we isolated and tested a yeast from the soil of sugarcane irrigated with vinasse, that is, with high mineral content and acidic pH. The strain of Meyerozyma caribbica URM 8365 was able to ferment glucose, but the use of xylose occurred when some oxygenation was provided. However, some fermentation of xylose to ethanol in oxygen limitation also occurs if glucose is present. This strain was able to produce ethanol from molasses substrate with 76% efficiency, showing its tolerance to possible inhibitors. High ethanol production efficiencies were also observed in acidic hydrolysates of each bagasse, sorghum, and cactus pear biomass. Mixtures of these substrates were tested and the best composition was found for the use of excess plant biomass in supplementation of primary substrates. It was also possible to verify the production of xylitol from xylose when the acetic acid concentration is reduced. Finally, the proposed metabolic model allowed calculating how much of the xylose carbon can be directed to the production of ethanol and/or xylitol in the presence of glucose. With this, it is possible to design an industrial plant that combines the production of ethanol and/or xylitol using combinations of primary substrates with hydrolysates of their biomass.

show abstract

Section: Discussionmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 99%

Meyerozyma Caribbica Isolated From Vinasse-Irrigated Sugarcane Plantation Soil: A Promising Yeast for Ethanol and Xylitol Production in Biorefineries

Alencar¹,

Freitas²,

Silva³

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…Indeed, xylose assimilation seems to be not an easy task for yeast cells. Pichia stipitis cells consumed around 66% of xylose after 72 h of fermentation [ 42 ] and M. caribbica consumed all xylose only after 120 h of cultivation [ 43 ], despite the experiments used in rich medium. On the other hand, Veras et al [ 44 ] reported high ethanol yields with full xylose consumption by Scheffersomyces stipitis and Spathaspora passalidarum in mineral medium under oxygen-limited conditions.…”

Section: Discussionmentioning

confidence: 99%

“…This organic acid can induce intracellular acidification that increases the demand for ATP and impairs the metabolic flux through the central metabolism [ 48 ]. The xylitol production by P. stipitis NCIM 3497 was also inhibited in the presence of 3.7 g/L of acetic acid [ 42 ]. Tadioto et al [ 22 ] also reported the impairment of xylose consumption and xylitol production by M. caribbica from stalk-straw corn hydrolysates that contained acetic acid above 3 g/L.…”

Section: Discussionmentioning

confidence: 99%

Meyerozyma caribbica Isolated from Vinasse-Irrigated Sugarcane Plantation Soil: A Promising Yeast for Ethanol and Xylitol Production in Biorefineries

Alencar

Freitas

Guimarães

et al. 2023

JoF

View full text Add to dashboard Cite

The production of fuels and other industrial products from renewable sources has intensified the search for new substrates or for the expansion of the use of substrates already in use, as well as the search for microorganisms with different metabolic capacities. In the present work, we isolated and tested a yeast from the soil of sugarcane irrigated with vinasse, that is, with high mineral content and acidic pH. The strain of Meyerozyma caribbica URM 8365 was able to ferment glucose, but the use of xylose occurred when some oxygenation was provided. However, some fermentation of xylose to ethanol in oxygen limitation also occurs if glucose was present. This strain was able to produce ethanol from molasses substrate with 76% efficiency, showing its tolerance to possible inhibitors. High ethanol production efficiencies were also observed in acidic hydrolysates of each bagasse, sorghum, and cactus pear biomass. Mixtures of these substrates were tested and the best composition was found for the use of excess plant biomass in supplementation of primary substrates. It was also possible to verify the production of xylitol from xylose when the acetic acid concentration is reduced. Finally, the proposed metabolic model allowed calculating how much of the xylose carbon can be directed to the production of ethanol and/or xylitol in the presence of glucose. With this, it is possible to design an industrial plant that combines the production of ethanol and/or xylitol using combinations of primary substrates with hydrolysates of their biomass.

show abstract

“…Most research work has been focused on studying ethanol production and hemicellulose conversion in combination with the fermentation inhibitors such as furfural, acetic acid, and hydroxymethylfurfural. Bhavana et al (2022), reported a synergistic effect of furfural, HMF, and acetic acid in reducing fermentation activity and xylitol production. Deshavath et al (2017), found that at low inhibitors concentrations, 78.6% and 82.8% of theoretical ethanol yield were obtained during fermentation of non-detoxified and detoxified hydrolysates.…”

Section: Effect Of Lignocellulosic Hydrolysate-inhibitors On Ethanol ...mentioning

confidence: 99%

Influence of furfural and acetic acid on bioenergetics of Scheffersomyces stipitis

Granados-Arvizu,

Saucedo-Gutierrez,

Escamilla-García

et al. 2023

Preprint

View full text Add to dashboard Cite

Lignocellulosic material is the most prominent carbon source to obtain profitable biotechnological processes, but compounds like furfural and acetic acid are highly toxic to yeasts. Nonetheless, research about the molecular mechanism of furfural and acetic acid toxicity is still scarce in yeasts like Scheffersomyces stipitis. Thus, this work aimed to identify the role of furfural and acetic acid on S. stipitis in bioenergetic and fermentation parameters. Here, we provide evidence that furfural and acetic acid caused a cell growth delay and extension of the lag phase. The mitochondrial membrane potential decreased in all treatments without differences among inhibitors or concentrations. Interestingly, the reactive oxygen species increased when the inhibitors concentrations were from 0.1 to 0.3% (v/v). The glycolytic flux was not significantly ( p>0.05) altered by acetic acid, but furfural caused different effects. The ethanol production decreased significantly (4.32 g/L in furfural and 5.06 g/L in acetic acid) compared to the control (26.3 g/L). At the same time, biomass was not significantly different in almost all treatments compared to the control. This study provides additional understanding of the effects of furfural and acetic acid at mitochondrial level in a pentose-fermenting yeast like S. stipitis.

show abstract

Effect of furfural, acetic acid and 5-hydroxymethylfurfural on yeast growth and xylitol fermentation using Pichia stipitis NCIM 3497

Cited by 14 publications

References 54 publications

Meyerozyma Caribbica Isolated From Vinasse-Irrigated Sugarcane Plantation Soil: A Promising Yeast for Ethanol and Xylitol Production in Biorefineries

Meyerozyma Caribbica Isolated From Vinasse-Irrigated Sugarcane Plantation Soil: A Promising Yeast for Ethanol and Xylitol Production in Biorefineries

Meyerozyma caribbica Isolated from Vinasse-Irrigated Sugarcane Plantation Soil: A Promising Yeast for Ethanol and Xylitol Production in Biorefineries

Influence of furfural and acetic acid on bioenergetics of Scheffersomyces stipitis

Contact Info

Product

Resources

About