Effect of gamma irradiation and its convergent treatment for control of postharvest Botrytis cinerea of cut roses

Abstract

Gamma irradiation and NaDCC introduced to control of gray mold on cut rose flowers. Integration of gamma irradiation and NaDCC inhibited the fungal development. Combined treatment can be applied to preserve the quality of the cut rose flowers. a b s t r a c tPostharvest diseases cause considerable losses to harvested crops. Among them, gray mold (Botrytis cinerea) is a major problem of exporting to cut rose flowers into Korea. Irradiation treatment is an alternative to phytosanitary purposes and a useful nonchemical approach to the control of postharvest diseases. Gamma irradiation was evaluated for its in vitro and in vivo antifungal activity against B. cinerea on cut rose varieties, 'Shooting Star' and 'Babe'. The irradiating dose required to reduce the population by 90%, D 10 , was 0.99 kGy. Gamma irradiation showed complete inhibition of spore germination and mycelial growth of B. cinerea, especially 4.0 kGy in vitro. Antifungal activity of gamma irradiation on rose B. cinerea is a dose-dependent manner. A significant phytotoxicity such as bent neck in cut rose quality was shown from gamma irradiation at over 0.4 kGy (po0.05) in both varieties. Although there is no significant difference in both varieties for fresh weight, in the case of flower rate, 'Babe' shows more sensitivity than 'Shooting Star'. In vivo assays demonstrated that established doses in in vitro, over 4 kGy, could completely inactive fungal pathogens, but such high doses can cause severe flowers damage. Thus, to eliminate negative impact on their quality, gamma irradiation was evaluated at lower doses in combination with an eco-friendly chemical, sodium dichloroisocyanurate (NaDCC) to examine the inhibition of B. cinerea. Intriguingly, only the combined treatment with 0.2 kGy of gamma irradiation and 70 ppm of NaDCC exhibited significant synergistic antifungal activity against blue mold decay in both varieties. Together, these results suggest that a synergistic effect of the combined treatment with gamma irradiation and NaDCC can be efficiently used to control the postharvest diseases in cut rose flowers, and will provide a promising technology for horticulture products for exportation.

Screening and partial purification of amylase from <i>Aspergillus Niger</i> isolated from deteriorated tomato (<i>Lycopersicon Esculentum</i> mill.) fruits

“…Adequate knowledge and careful handling procedure of the tomatoes can reduce wastage of the fruits (10). Previous study on deterioration of tomatoes by microorganism showed that post-harvest damages are mostly due to attack by fungi such as Aspergillus niger, Aspergillus flavus and Rhizopus stolonifer of up to 90% prevalence (7,11). Thus, there is need for constant research to isolate the fungi associated with tomato fruits deterioration with the view to providing suitable solutions of preserving the tomato fruits, keeping them fresh up to when they reach the consumers (12).…”

Prolongation of the Shelf-Life of Strawberry Fruits by the Reduction of the Gray Mold; Botrytis cinerea using Gamma Irradiation and/or Chitosan

“…Generally, it was previously established that besides being active in maintaining the quality and expanding the shelf-life of fresh fruits, chitosan also prevent microbial damage, healthy safe and effective coating agent, (Shiekh et al 2013) Moreover coating fruits by chitosan reduces water loss and maintains the quality of the strawberry such as color, titratable acidity, and vitamin C. Such study reported enhancements in the antioxidant activity of some enzymes, as well as stopping in the flesh browning and reduction of fruit damage as a result of chitosan coating (Petriccione et al 2015) The in vitro and in vivo assessment of gamma irradiation as an antifungal agent against B. cinereal, revealed that 99kGy reduced the fungal intensity by 90%. At 4.0kGy of gamma irradiation completely inhibited the germination of spores and the growth of mycelia (Chu et al 2015). Hussain et al, (2013) studied the combinatory treatment as a synergistic effect to maximize the GI action, in this connection, the modified atmosphere, heat, and washing effectively extended the shelf-life of the fruits.…”

NaDCC) and sodium hypochlorite (NaOCL): Modeling, optimization and comparative analysis

“…They concluded that higher disinfection efficiency by NaDCC may be originated from higher germicidal power, slower decomposition and HOCl releasing in the longer time, higher capacity for maintaining available free chlorine, and more resistance to pH changing [20,21]. Also, a number of studies have been conducted for assessing effect of NaDCC on Streptococcus sobrinus, Strep-tococcus salivarius, Enterococcus faecalis, Streptococcus mutans [22], Clostridium difficile spores [23], and Botrytis cinerea [24]. So far, however, there has been a little discussion about the impact of NaDCC on gram-negative bacteria.…”