Effect of gamma irradiation on the osteoinductivity of morphogenetic protein extract from reindeer bone

Pekkarinen, Tarmo; Hietala, Oili A.; Jämsä, Timo; Jalovaara, P.

doi:10.1080/00016470510030625

Cited by 11 publications

(15 citation statements)

References 17 publications

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“…Until now, there have been no studies investigating the ability of reindeer BMP extract to heal long bone defects. We have shown previously that reindeer BMP extract is an effective inducer of new bone formation in a muscle pouch model in mice (Pekkarinen et al 2003(Pekkarinen et al , 2004(Pekkarinen et al , 2005b. In the present study, we compared the bone healing properties of the reindeer BMP extract to results with untreated and collagen controls, while rhBMP-2 (InductOS) was also included in the comparisons because the bone healing capacity of rhBMP-2 has been well documented in experimental animal and clinical studies (Hollinger et al 1998, Sciadini and Johnson 2000, Yudell and Block 2000, Govender et al 2002, Li et al 2002.…”

Section: Discussionmentioning

confidence: 99%

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Reindeer BMP extract in the healing of critical-size bone defects in the radius of the rabbit

Pekkarinen¹,

Jämsä

Määttä

et al. 2006

Acta Orthopaedica

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Background Native BMP extracts from reindeer effectively induce ectopic new bone formation in vivo, but their bone healing properties have not yet been evaluated. We investigated the effect of reindeer BMP extracts on the healing of long bone defects.Methods The implants tested contained 5 mg or 10 mg of unsterilized BMP extract from reindeer and 10 mg of gamma-sterilized BMP extract administered with collagen carrier (Lyostypt, B. Braun, Germany). 70 µg of rhBMP-2 with collagen carrier (InductOs; Wyeth Europa) served as positive control, and collagen implants (Lyostypt) and untreated defects served as negative controls. New Zealand White rabbits with 1.5 cm of critical-size radius bone defects were used, with 8 weeks of follow-up.Results Radiographic analysis showed bone formation (BF) to be higher in all groups containing BMPs than in the untreated controls. BF was also higher in the rhBMP-2 group, and marginally higher in the group treated with 10 mg of unsterilized reindeer BMP extract (p = 0.06) as compared to the collagen controls. Bone union (BU) was better in the unsterilized BMP extract groups and rhBMP-2 group than in the untreated controls. BU was also better in the implants with 10 mg of unsterilized reindeer BMP extract and rhBMP-2 than in the collagen-treated implants. The mean area of new bone at the site of the defect proved to be higher in all implants containing BMP than in the untreated defects. It was also higher in the groups with 10 mg of unsterilized reindeer BMP extract and rhBMP-2 than in the collagen-treated controls. Mechanical tests showed torsional stiffness of the bones to be higher in the group with 10 mg of unsterilized BMP extract than in the collagen

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Section: Discussionmentioning

confidence: 99%

“…The doses were chosen according to our previous studies (Pekkarinen et al 2003(Pekkarinen et al , 2004(Pekkarinen et al , 2005b(Pekkarinen et al , 2005c. Finally, implants were lyophilized in test tubes for 48 h.…”

Section: Reconstitution Of Implants and Sterilizationmentioning

confidence: 99%

Reindeer BMP extract in the healing of critical-size bone defects in the radius of the rabbit

Pekkarinen¹,

Jämsä

Määttä

et al. 2006

Acta Orthopaedica

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“…In some studies, irradiation has been found to inhibit (Buring and Urist 1967) or only partially destroy (Munting et al 1988, Schwarz et al 1988, Ijiri et al 1994, Zhang et al 1997) the osteoinductive capacity. In others, no effect of irradiation on the osteoinductive capacity has been found (Wientroub and Reddi 1988, Andriano et al 2000, Ripamonti et al 2000, Pekkarinen et al 2005.…”

Section: Histomorphometrymentioning

confidence: 99%

“…These proteins may be released during the resorptive phase of bone graft incorporation, and can have a profound effect on bone cell physiology. Although studies have been performed to determine the effect of irradiation on BMPs (Munting et al 1988, Wientroub and Reddi 1988, Dziedzic-Goclawska et al 1991, Ijiri et al 1994, Andriano et al 2000, Ripamonti et al 2000, Pekkarinen et al 2005, the effect of irradiation on the incorporation process of morselized bone chips remains unclear. We studied the effects of steri-lization by gamma irradiation on the incorporation of impacted morselized allografts.…”

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confidence: 99%

Irradiation has no effect on the incorporation of impacted morselized bone: A bone chamber study in goats

2007

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Background Gamma irradiation has been widely used for sterilization of bone allografts. However, gamma irradiation alters proteins. This is favorable when it reduces immunogenicity, but is undesirable when osteoinductive proteins are damaged. Although the effect of gamma irradiation on BMPs has been studied, the effect of irradiation on the process of incorporation of morselized bone chips remains unclear. We studied the effects of sterilization by gamma irradiation on the incorporation of impacted morselized allografts.Methods Bone chambers with impacted allografts, rinsed impacted allografts, allografts that were rinsed and subsequently irradiated, and an empty control were implanted in proximal medial tibiae of goats. Incorporation was evaluated using histology and histomorphometry.Results Histology revealed evidence of bone graft incorporation, which proceeded in a similar way in unprocessed, rinsed, and both rinsed and irradiated bone grafts. After 12 weeks, no difference in bone and tissue ingrowth was found between the unprocessed, the rinsed, and the rinsed and subsequently irradiated allografts. The amount of unresorbed graft remnant was highest in the unprocessed bone grafts.Interpretation We conclude that sterilization with gamma irradiation does not influence the incorporation of impacted rinsed bone allografts.

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“…Radiation exposure induces latent TGF-β activation (25,26). Other studies reported that the osteoinductive potential of bone morphogenetic proteins of various origins was preserved after sterilization at 25 kGy (27,28). Growth factors react differently to gamma irradiation: gamma sterilization alters the properties of insulin-like growth factor-1 (29), whereas the TGF-β superfamily is resistant to high doses of radiation.…”

Section: Discussionmentioning

confidence: 99%

High-dose irradiation of bone chips preserves the <i>in vitro</i> activity of bone-conditioned medium

et al. 2016

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Extracorporeal irradiation sterilizesresected tumor bone used as autografts in reconstruction surgery. Therapeutic irradiation is a standard technique in head and neck cancer therapy that aims to preserve organ function. Bone irradiation has a complex, mostly inhibitory, effect on remodeling and regeneration, although the underlying mechanisms are still not fully understood. It remains unclear if extracorporeal irradiation affects the paracrine-like activity of the corresponding autografts. We recently reported that bone-conditioned medium from autogenous bone chips contains a number of factors that might affect cell activity. In the present study, we investigated the effects of extracorporeal irradiation of porcine cortical bone chips on the activity of the corresponding bone-conditioned medium. The effects of bone-conditioned medium on the expressions of transforming growth factor-beta (TGF-β) target genes in oral fibroblasts were assessed. Bone-conditioned medium from bone chips exposed to a total radiation dose up to 120 Gy did not affect expressions of TGF-β target genes, including adrenomedullin, BTB/ POZ domain-containing protein 11, proteoglycan 4, NADPH oxidase 4, and interleukin 11, in oral fibroblasts. In conclusion, bone irradiation does not alter the capability of the corresponding bone-conditioned medium to provoke a robust fibroblastic cell response in vitro. (J Oral Sci 58, 325-331, 2016)

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Effect of gamma irradiation on the osteoinductivity of morphogenetic protein extract from reindeer bone

Cited by 11 publications

References 17 publications

Reindeer BMP extract in the healing of critical-size bone defects in the radius of the rabbit

Reindeer BMP extract in the healing of critical-size bone defects in the radius of the rabbit

Irradiation has no effect on the incorporation of impacted morselized bone: A bone chamber study in goats

High-dose irradiation of bone chips preserves the <i>in vitro</i> activity of bone-conditioned medium

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