Effect of gamma radiation on seed germination and seedling growth of snake gourd (Trichosanthes anguina L.)

Layek, Santanu; Pramanik, Subhradeep; Das, Arindam; Gupta, Akhilesh; Bhunia, Arindam; Pandit, M. K.

doi:10.1016/j.sajb.2021.07.039

Cited by 7 publications

(4 citation statements)

References 5 publications

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“…At doses lower than GR 50 , desirable effects and variations may not be obtained. Also, higher doses might cause deleterious effects on the plants causing damage to the plant cells and the plant population per treatment was also affected (Layek et al, 2021). Hence, it is inferred from the present study that for getting effective genetic varia- 4 and 5), the mutagenic effectiveness and efficiency were found since they are the indices for the mutability of a cultivar.…”

Section: Discussionmentioning

confidence: 59%

Efficiency of gamma irradiation and ethyl methane sulphonate in inducing variations in Jasminum auriculatum Vahl.

Lavanya

Ganga²,

Rajamani³

et al. 2022

JANS

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Vegetatively propagated crops like Jasminum auriculatum have a constraint in genetic variation due to a narrow genetic base that restricts the insights in any crop breeding programme. Mutation breeding is a potential tool that directs a way to create desirable variation effectively in vegetatively propagated crops. The optimum dose of mutagen is the one which produces the maximum frequency of mutation in turn the variation, with minimum killing. The aim of the present paper unveils the Lethal Dose (LD50) and the growth reduction dose (GR50) for both Gamma irradiation (GI) and Ethyl methane sulphonate (EMS) for the ecotype ‘Muthu Mullai’ of Jasminum auriculatum. These parameters of GR and EMS treated cuttings were analysed by considering the GR50 values of the mortality rate (57%, 48%), survival percentage (12.479Gy, 13.268mM), shoot length (18.59Gy, 18.28mM), root length (20.39Gy, 18.17mM), number of leaves (22.29Gy, 17.47mM), number of sprouts (22.97Gy, 16.17mM), vigour index (10.43Gy, 11.05mM), leaf length (21.61Gy, 19.90mM) and leaf width (19.2Gy, 16.17mM). The LD50 value was 12.479 Gy for GI and 13.268 mM for EMS treatment. The GR50 for different growth parameters ranged from 14.93 to 22.9 Gy for GI and 1.05 to 19.9 mM for EMS treatment. The mutagenic efficiency and effectiveness were 214.96 and 89.36 for GI and 48.66 and 33.77 for EMS treatment, respectively. These doses can be used for generating considerable variation, which can be put to use in future crop improvement programmes for Jasmine.

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Section: Discussionmentioning

confidence: 59%

Efficiency of gamma irradiation and ethyl methane sulphonate in inducing variations in Jasminum auriculatum Vahl.

Lavanya

Ganga²,

Rajamani³

et al. 2022

JANS

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“…The determined GR 50 value in this research, through probit analysis, was 217.2 Gy. In general, GR 50 is an effective method for quantifying a parameter’s 50% decrease [ 23 ]. Because M 1 plants are heterozygous at the genotypic level, the change caused by mutation may be seen in any allele located in any random locus, leading to reduced mutation manifestation [ 36 ].…”

Section: Discussionmentioning

confidence: 99%

“…As a result, this research was undertaken to find the optimal dosage of acute gamma-irradiated mutagenesis for the improvement of lentil’s agronomically significant features to achieve a greater mutation frequency [ 22 , 23 ]. Additionally, the immediate impact of gamma irradiation on seedling and sterility parameters is discussed in this section.…”

Section: Introductionmentioning

confidence: 99%

Morphometric frequency and spectrum of gamma-ray-induced chlorophyll mutants identified by phenotype and development of novel variants in lentil (Lens culinaris Medik.)

Pramanik¹,

Debnath²,

Rahimi³

et al. 2023

PLoS ONE

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Genetic variations are a crucial source of germplasm heterogeneity, as they contribute to the development of new traits for plant breeding by offering an allele resource. Gamma rays have been widely used as a physical agent to produce mutations in plants, and their mutagenic effect has attracted much attention. Nonetheless, few studies have examined the whole mutation spectrum in large-scale phenotypic evaluations. To comprehensively investigate the mutagenic effects of gamma irradiation on lentils, biological consequences on the M1 generation and substantial phenotypic screening on the M2 generation were undertaken. Additionally, the study followed the selected mutants into the M3 generation to evaluate the agronomic traits of interest for crop improvement. Seeds of lentil variety Moitree were irradiated with a range of acute gamma irradiation doses (0, 100, 150, 200, 250, 300, and 350 Gy) to induce unique genetic variability. This research focused on determining the GR50 value while considering seedling parameters and examining the status of pollen fertility while comparing the effects of the gamma irradiation dosages. The GR50 value was determined to be 217.2 Gy using the seedling parameters. Pollens from untreated seed-grown plants were approximately 85% fertile, but those treated with the maximum dosage (350 Gy) were approximately 28% fertile. Numerous chlorophyll and morphological mutants were produced in the M2 generation, with the 300 Gy -treated seeds being the most abundant, followed by the 250 Gy -treated seeds. This demonstrated that an appropriate dosage of gamma rays was advantageous when seeking to generate elite germplasm resources for one or multiple traits. Selected mutants in the M3 generation showed improved agronomic traits, including plant height, root length, number of pods per plant, and yield per plant. These investigations will contribute to a comprehensive understanding of the mutagenic effects and actions of gamma rays, providing a basis for the selection and design of suitable mutagens. This will facilitate the development of more controlled mutagenesis protocols for plant breeding and help guide future research directions for crop improvement using radiation-induced mutation breeding techniques.

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“…The effective dose, expressed as LD 50 , induced a scaling down by 50% of the good seed viability. In addition to the LD 50 , there is the RD 50 , a dose that reduces the viability of seeds of the M1 population by 50% (Layek et al 2022). Calculating the lethal dose of 50% is a standard method for obtaining mutants; it is based on the fact that there is a balance between physiological damage and acquired genetic changes.…”

Section: Discussionmentioning

confidence: 99%

The Changes in Seed Germination Capacity, Seedling Growth, and Leaf Morphology of Ficus variegata Blume Influenced by Gamma-Ray Irradiation

Zanzibar¹,

Pramono²,

Yulianti³

et al. 2022

HAYATI J Biosci

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Gamma irradiation treatments have commonly been applied to induce variation in plant genetic improvement programs. The improvement of nyawai (Ficus variegata Blume) is urgently needed mainly to break the seed dormancy, increase seedling growth, and induce variation for selecting desired traits. This research aimed to assess the effect of gamma irradiation doses on seed germination, seedling growth, and leaf morphology of Ficus variegate Blume. This experiment's irradiation doses ranged from 5-240 Gy and were applied to seeds with varying levels of vigor based on the artificial seed aging process. The data displayed a positive relationship between low doses of gamma irradiation and the nyawai seed's endurance. Low irradiation doses, 5 to 30 Gy, are deemed capable of promoting seedling development, leaf retention, and diversity in leaf structure. The higher irradiation doses than 30 Gy decreased seedling growth, leaf number, and seedling survival. In addition, these doses also affect some morphological changes, including seedling height and leaf length and width.

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Effect of gamma radiation on seed germination and seedling growth of snake gourd (Trichosanthes anguina L.)

Cited by 7 publications

References 5 publications

Efficiency of gamma irradiation and ethyl methane sulphonate in inducing variations in Jasminum auriculatum Vahl.

Efficiency of gamma irradiation and ethyl methane sulphonate in inducing variations in Jasminum auriculatum Vahl.

Morphometric frequency and spectrum of gamma-ray-induced chlorophyll mutants identified by phenotype and development of novel variants in lentil (Lens culinaris Medik.)

The Changes in Seed Germination Capacity, Seedling Growth, and Leaf Morphology of Ficus variegata Blume Influenced by Gamma-Ray Irradiation

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