Effect of gamma-ray on activity and stability of alcohol-dehydrogenase from Saccharomyces cerevisiae

Immobilization of Saccharomyces cerevisiae alcohol dehydrogenase on hybrid alginate–chitosan beads

“…Li et al [5] have reported similar results when SCAD was immobilized on magnetic Fe 3 O 4 -chitosan nanoparticles. Similar reports on increase in optimum temperature during enzyme immobilization have been previously reported [2,13,16,22,24]. The shift in optimal temperature toward higher temperature might be due to the immobilization of the enzyme to the support, providing stability and resulting in formation of the enzyme-substrate complex without any hindrance for the access of substrates to the active site [16,26].…”

“…After sixth cycle, hybrid alginate-chitosan beads were disintegration and there was enzyme denaturing due to physical loss of enzyme. In Ben Akacha's report [2], when the whole cell alcohol dehydrogenase was immobilized onto alginate gel beads, the immobilized preparation maintains 70% of its initial activity after the fourth cycle, and this activity continued to decrease steadily reaching a minimum of 10% after seven cycles. To a certain extent, the hybrid alginate-chitosan beads have a little good operational stability.…”

“…The immobilized enzyme by entrapment showed greater stability at higher temperature than its free counterpart, which was more stable at lower temperatures. The actual temperature in the microenvironment of the immobilized enzyme is lower than that in the bulk of the medium [2]. However, higher temperature may lead to higher diffusion limitations so that the SCAD activity decreased up to 40 • C. …”

“…It shows good capacity as a redox biocatalyst in a variety of stereoselective reductions and in the regeneration of coenymes NADH in vivo continuously [1][2][3]. However, the application of free enzyme is often hampered by its reusability because free enzyme is lack of long term stability under processing conditions and difficult to be recycled from the reaction mixture.…”

Surface functionalization of chitosan-coated magnetic nanoparticles for covalent immobilization of yeast alcohol dehydrogenase from Saccharomyces cerevisiae

“…Generally, Saccharomyces cerevisiae is a good source for this enzyme. In spite of the wide range of applications and interests, YADH is very sensitive and has poor stability in aqueous solutions, which limits its application [20]. YADH immobilization could offer many advantages with the possibility of continuous processing, reusing of the enzyme, and reduction of auto-digestion.…”