Effect of garlic and vitamin B-complex in lead acetate induced toxicities in mice

Abstract

The experiment was performed on 25 adult mice, (Swiss albino), weighing about 25 gm, maintained on standard pellet diet and drinking water ad libitum. All the mice were randomly divided into five equal groups (5x5). Each group comprised of five mice was marked as group A, B, C, D and E. In the present study an attempt has been taken to study the effect of Garlic and vitamin B-complex in lead induced toxicities in mice with the observation of some parameters as toxic signs and body weight, some haematological parameters like total erythrocyte count (TEC), total leukocyte count (TLC), haemoglobin content (Hb%) and Packed cell volume (PCV), some biochemical parameters such as serum glutamate pyruvate transaminase SGPT/ALT, Serum glutamate Oxaloacetate transaminase (SGOT/AST) and postmortem changes in mice. An attempt was also made to estimate the residual deposition of lead in body tissues i.e. blood, liver, kidney, brain and bone in mice. Mice treated with lead acetate showed severe toxic signs and significantly reduced total erythrocyte count, total leukocyte count, haemoglobin content and packed cell volume and significant elevation of SGPT and SGOT. But in case of mice treated by lead acetate along with Garlic and vitamin B-complex showed almost normal levels of haematological and biochemical parameters. From this experiment it is revealed that Garlic and vitamin B-complex has protective and curative effect in lead toxicity. The mean body weight of mice of group B (only lead acetate) was significantly (p<0.01) decreased, in comparison with the other treated groups. Lead acetate group also significantly reduced (p<0.01) total erythrocyte count, packed cell volume and hemoglobin content was on 42 days of treatment. SGPT and SGOT were significantly elevated in all treated groups in comparison with control group. The liver of the mice of group B were blackish discoloration and enlarged. The significant reduction of lead in blood, liver and kidney was found in group E<C<D in comparison to group B (lead treated). The order of effectiveness was Garlic + Vitamin B-complex> Garlic> Vitamin Bcomplex.

Effect of calcium supplementation on lead-induced genotoxicity and bioaccumulation in suckling Wistar rats

“…The change in the body weight of the sucklings exposed to Pb only was lower than that of the other groups. Our findings on the effect of lead on the body weight are in line with previous studies in which reduced weight gains after intoxication with lead acetate in adult mice (Khan et al 2008) and rats (Szymezak, Zechalko and Biernat 1983) have been reported. This supports the hypothesis that Pb does influence growth during childhood.…”

Protective Effects of Wheat Sprout on Testicular Toxicity in Male Rats Exposed to Lead

“…In the current study, prescription of combined vitamin E and lead resulted a significant increase in parameters including seminiferous tubules diameter, number of Sertoli cells and motility and viability of sperms. Positive effects of vitamins E, C, folic acid, thiamin, niacin and B 12 have been reported in genital system and sperm fertility parameters in males exposed to lead [5][6][7]47,48]. Another determinant of cell damage by lead is ALP enzyme.…”

Does allicin combined with vitamin B-complex have superior potentials than alpha-tocopherol alone in ameliorating lead acetate-induced Purkinje cell alterations in rats? An immunohistochemical and ultrastructural study

“…Previous studies have discussed the ameliorative role of combined Allium sativum and vitamin B-complex against lead acetate-induced toxicity and have proved the potency of these combined substances [27]. It has been found that allicin role is not only ameliorative against lead toxicity, but the plant also contains chelating compounds capable of enhancing the elimination of lead [36].…”