Effect of Gelatins on Human Cancer Cells In Vitro

Abstract

Three types of gelatins were tested for their antiproliferative activities in vitro against three human tumor cell lines (K-562; erythroleukemia, HCT-15; colon carcinoma, AGS; gastric carcinoma) with viable cell count and tritium-thymidine ((3)H-TdR) uptake by those cells. Porcine skin (PS) gelatin exerted the strongest antiproliferative activity of all three gelatins. Bovine bone (BB) gelatin did not exert such an activity. PS gelatin exerted antiproliferative activity against K-562 cells also in a serum-free medium. The serum-free medium contains two growth factors, insulin and transferrin, as well as nutrients. The activity of PS gelatin was not interfered by addition of insulin and transferrin to the medium. Effect of diluting a K-562 cell-concentration on the activity of PS gelatin was tested. Diluting the cell concentration did not affect the activity of PS gelatin. Moreover, the conditioned medium in which K-562 cells had been cultured did not stimulate the proliferation of K-562 cells. In conclusion, PS gelatin suppress the proliferation of human tumor cell lines in vitro. The antiproliferative activity of PS gelatin might not be attributed to trapping growth factors or autocrine mediators.

Collagens, stromal cell‐derived factor‐1α and basic fibroblast growth factor increase cancer cell invasiveness in a hyaluronan hydrogel

“…Because MMP-2 and MMP-9 are known to be implicated in proteolysis of types IV and V collagens (Nabeshima et al 2002) that constitutes blood vessel basement membrane, our results suggest that HA and collagen may play an active role in tumour cell intra-and extravasation determining the metastatic process. On the contrary, increase of invasiveness induced by gelatin on the CB191 cell line could involve other signalling events, because the RGD peptides (arginine-glycine-aspartic acid) of gelatin are exposed to integrins without activation of MMP (Kojima et al 2003).…”

Entry of Influenza A Virus with a α2,6-Linked Sialic Acid Binding Preference Requires Host Fibronectin

“…FN uses its synergistic adhesion site and Arg-Gly-Asp motifs within the cell-binding domain to bind to this protein for cell membrane attachment (2). To investigate whether ␣5␤1 integrin is directly involved in the postattachment event of WSN virus, two ␤1-integrin-specific antibodies (AIIB2 and N20) were tested individually in the MDCK and A549 cell models (21,27). Both of these antibodies were tested at a concentration known to inhibit biological functions of ␣5␤1 integrin in cell cultures.…”

3D culture of bovine articular chondrocytes in viscous medium encapsulated in agarose hydrogels for investigation of viscosity influence on cell functions

“…However, different concentrations of polymers may affect cell behaviors. 37 Furthermore, if the polymers such as gelatin have bio-active groups, variation of the polymer concentration can change the amount of the bio-active groups in the medium leading to undesired interference on the results. 38,39 Therefore, the sole variation of the viscosity without variation of the polymer concentration and the bioactive moiety is desirable for the investigation of the viscosity effect on cell functions.…”