Effect of genetic background and activating stimulus on the timing of meiotic cell cycle progression in parthenogenetically activated mouse oocytes

Ibáñez, Elena; Albertini, David F.; Overström, E.W.

doi:10.1530/rep.1.00452

Cited by 21 publications

(9 citation statements)

References 53 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In our preliminary experiments, we have confirmed the difficulty in activating 129 strain oocytes by strontium: many of them arrested at metaphase II (35%, 6/17) or metaphase III (47%, 8/17), and only 18% (3/17) developed to 2-cells (unpublished). Although it was reported that C57BL/6 strain oocytes also often arrested at metaphase III after parthenogenetic development [42], ROSI-generated embryos in this study developed into 2-cell embryos at normal rates (45–89%).…”

Section: Discussionmentioning

confidence: 52%

The Effect on Intracytoplasmic Sperm Injection Outcome of Genotype, Male Germ Cell Stage and Freeze-Thawing in Mice

et al. 2010

View full text Add to dashboard Cite

BackgroundIntracytoplasmic sperm injection (ICSI) has been widely used to study the mechanisms of mammalian fertilization and to rescue male-factor infertility in humans and animals. However, very few systematic analyses have been conducted to define factors affecting the efficiency of ICSI. In this study, we undertook a large-scale series of ICSI experiments in mice to define the factors that might affect outcomes.Methodology/Principal FindingsWe used a 5×3×2 factorial design with the following factors: mouse genotype (ICR, C57BL/6, DBA/2, C3H/He, and 129/Sv strains), type of male germ cells (epididymal sperm, elongated or round spermatids), and their freeze–thawing treatment. The efficiencies (parameters) of each developmental step were analyzed by three-way ANOVA (significance level P<0.01). The type of male germ cells affected all the four parameters observed: oocyte survival after injection, cleavage of oocytes, implantation, and birth of offspring. Genotype affected the oocyte survival, cleavage and birth rates, whereas freeze–thawing had no effects on any of the parameters. There were significant genotype/cell type interactions for oocyte survival and cleavage, indicating that they were determined by a combination of strain and germ cell maturity. Multiple comparisons revealed that spermatozoa and elongated spermatids gave better implantation and birth rates than did round spermatids, while spermatozoa and elongated spermatozoa were indistinguishable in their ability to support embryonic development. The best overall efficiency (birth rate per oocytes injected) was obtained with frozen–thawed DBA/2 strain elongated spermatids (23.2±4.2%).Conclusions/SignificanceThe present study provides the first comprehensive information on ICSI using the mouse as a model and will contribute to the efficient use of materials, time, and efforts in biomedical research and clinics involving ICSI.

show abstract

Section: Discussionmentioning

confidence: 52%

The Effect on Intracytoplasmic Sperm Injection Outcome of Genotype, Male Germ Cell Stage and Freeze-Thawing in Mice

et al. 2010

View full text Add to dashboard Cite

show abstract

“…While the general activities of SKI606 and PP2 are similar, slight differences in affinity and selectivity may account for the varied effects reported here and elsewhere. More likely, variations in chemical inhibitor results may take their origins in IVM culture protocols and/or differences in mouse strains used since mouse strain variations have been reported for both in vivo and in vitro matured oocytes (Ibanez et al, 2005a; Ibanez et al, 2005b). Additionally, Zheng and colleagues (2007) cultured naked oocytes in basal M2 medium without supplementation.…”

Section: Discussionmentioning

confidence: 99%

Functions of Fyn kinase in the completion of meiosis in mouse oocytes

McGinnis

Kinsey

Albertini

2009

Developmental Biology

View full text Add to dashboard Cite

Oocyte maturation invokes complex signaling pathways to achieve cytoplasmic and nuclear competencies for fertilization and development. The Src-family kinases FYN, YES and SRC are expressed in mammalian oocytes but their function during oocyte maturation remains an open question. Using chemical inhibitor, siRNA knockdown, and gene deletion strategies the function of Src-family kinases was evaluated in mouse oocytes during maturation under in vivo and in vitro conditions. Suppression of Src-family as a group with SKI606 greatly reduced meiotic cell cycle progression to metaphase-II. Knockdown of FYN kinase expression after injection of FYN siRNA resulted in an approximately 50% reduction in progression to metaphase-II similar to what was observed in oocytes isolated from FYN (−/−) mice matured in vitro. Meiotic cell cycle impairment due to a Fyn kinase deficiency was also evident during oocyte maturation in vivo since ovulated cumulus oocyte complexes collected from FYN (−/−) mice included immature metaphase-I oocytes (18%). Commonalities in meiotic spindle and chromosome alignment defects under these experimental conditions demonstrate a significant role for Fyn kinase activity in meiotic maturation.

show abstract

“…Although two recent studies performed in-depth analysis of allelic gene expression during embryonic development, these studies focused on much later stages (from E9.5 onward) [11,20]. Furthermore, previous studies were carried out on a variety of mouse genetic backgrounds, which is known to influence the efficiency of ESC derivation and to affect NT and PGA [29][30][31][32]. In our study, we make use of isogenic F1 hybrids of C57BL/6J (maternal) and DBA/2J (paternal) mouse inbred strains, previously also referred to as B6D2F1, B6D2 or BDF1.…”

Section: Introductionmentioning

confidence: 99%

“…In our study, we make use of isogenic F1 hybrids of C57BL/6J (maternal) and DBA/2J (paternal) mouse inbred strains, previously also referred to as B6D2F1, B6D2 or BDF1. This cross has been shown to be relatively efficient in NT and PGA, and the use of isogenic tissues and cell lines eliminates complications arising from strain-specific differences in gene expression [30,31,33]. Importantly, when derived from hybrid F1 mice, ESC-PGAs contain a mosaic homo-and heterozygous genotype due to chromosomal crossover occurring in the parental B6D2F1 oocyte during meiosis [10].…”

Section: Introductionmentioning

confidence: 99%

Allele-specific RNA-seq expression profiling of imprinted genes in mouse isogenic pluripotent states

Dirks

Mierlo

Kerstens

et al. 2019

Epigenetics & Chromatin

View full text Add to dashboard Cite

Background: Genomic imprinting, resulting in parent-of-origin specific gene expression, plays a critical role in mammalian development. Here, we apply allele-specific RNA-seq on isogenic B6D2F1 mice to assay imprinted genes in tissues from early embryonic tissues between E3.5 and E7.25 and in pluripotent cell lines to evaluate maintenance of imprinted gene expression. For the cell lines, we include embryonic stem cells (ESCs) and epiblast stem cells (EpiSCs) derived from fertilized embryos and from embryos obtained after nuclear transfer (NT) or parthenogenetic activation (PGA). Results: As homozygous genomic regions of PGA-derived cells are not compatible with allele-specific RNA-seq, we developed an RNA-seq-based genotyping strategy allowing identification of informative heterozygous regions. Global analysis shows that proper imprinted gene expression as observed in embryonic tissues is largely lost in the ESC lines included in this study, which mainly consisted of female ESCs. Differentiation of ESC lines to embryoid bodies or NPCs does not restore monoallelic expression of imprinted genes, neither did reprogramming of the serum-cultured ESCs to the pluripotent ground state by the use of 2 kinase inhibitors. Fertilized EpiSC and EpiSC-NT lines largely maintain imprinted gene expression, as did EpiSC-PGA lines that show known paternally expressed genes being silent and known maternally expressed genes consistently showing doubled expression. Notably, two EpiSC-NT lines show aberrant silencing of Rian and Meg3, two critically imprinted genes in mouse iPSCs. With respect to female EpiSC, most of the lines displayed completely skewed X inactivation suggesting a (near) clonal origin. Conclusions: Altogether, our analysis provides a comprehensive overview of imprinted gene expression in pluripotency and provides a benchmark to allow identification of cell lines that faithfully maintain imprinted gene expression and therefore retain full developmental potential.

show abstract

Effect of genetic background and activating stimulus on the timing of meiotic cell cycle progression in parthenogenetically activated mouse oocytes

Cited by 21 publications

References 53 publications

The Effect on Intracytoplasmic Sperm Injection Outcome of Genotype, Male Germ Cell Stage and Freeze-Thawing in Mice

The Effect on Intracytoplasmic Sperm Injection Outcome of Genotype, Male Germ Cell Stage and Freeze-Thawing in Mice

Functions of Fyn kinase in the completion of meiosis in mouse oocytes

Allele-specific RNA-seq expression profiling of imprinted genes in mouse isogenic pluripotent states

Contact Info

Product

Resources

About