Effect of Genistein Supplementation in Tris-Aminomethane Base Extender on Characteristics of Pre-Freezing and Post-Thawed Sperm Ongole Grade Bull

Abstract

Sperm undergo physiological changes during the cooling and freezing process, and especially significant changes are seen after freezing than after cooling. This research analyzed the effect of genistein supplementation in the basic diluent of tris-aminomethane on the quality of spermatozoa. Bulls aged 3-4 years used in this research. This research used an experimental method to examine the effect of genistein levels in the diluent on the characteristics of spermatozoa during pre-freezing at 4-5 0 C and post-thawing (PT). The data obtained in the form of quantitative data were analyzed descriptively and analysis of variance patterns in One-Way ANOVA. 0 µM, 10 µM, 30 µM and 50 µM genistein treatment in the basic diluent tris aminomethane-egg yolk. Progressive motility post-thawing at 1 hour and 2 hours was highest at genistein concentration 30 µM (P<0.05). The effect of the antioxidant genistein on tris aminomethane -egg yolk diluent gave a significant effect on sperm viability in the Before Freezing (BF) phase at 1.5 hours, BF and PT at two hours (P<0.05). The addition of different genistein in diluent tris aminomethane -egg yolk was no significant effect (P>0.05) on sperm abnormality in both the BF and PT phases at 1 hours, 1.5 hours and 2 hours. The addition of genistein with a concentration of 30 µM was able to maintain better semen quality.