Effect of genotype on in vitro adventitious shoot formation in Pinus radiata and correlations between pairs of phenotypic traits during in vitro shoot development

Bergmann, Ben A.; Stomp, Anne-Marie

doi:10.1007/bf00035969

Cited by 9 publications

(1 citation statement)

References 11 publications

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“…The influence of genotype and family on in vitro traits has been reported for other pine species (for review see Bergmann and Stomp 1994). However, generally speaking, very few studies on conifers have taken into account the performance of the mother tree.…”

Section: Discussionmentioning

confidence: 99%

Relationship between field performance, family, embryo morphology, and isozyme heterozygosity, and in vitro reactivity in jack pine

Briand¹,

Gauthier²,

Lemay³

et al. 1998

Can. J. For. Res.

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The influence of field performance, family, embryo morphology, and isozyme heterozygosity level on in vitro reactivity of Pinus banksiana Lamb. was evaluated on embryos from five superior families, five inferior families, and a mixed seed lot. Embryo length, number of cotyledons, and isozyme heterozygosity were determined for each embryo. Seed germination and fresh weight were determined on a family level. On average, superior families showed higher percentages of embryos that formed buds in vitro. Within each performance class, the analysis based on initial number of embryos revealed differences among families for the percentage of green embryos and embryos with adventitious buds and shoots. When calculations were based on green embryos only, i.e., excluding embryos that remained white, there were no differences among families. Thus, the overall in vitro potential of a family appears to be strongly dependent upon the capacity of embryos to turn green. On a per family basis, seed germination was positively correlated with most in vitro characters, with the exception of mean shoot length per shoot-forming embryo. Small embryos had a lower probability of producing buds and shoots, and embryos with three cotyledons showed a higher mortality than embryos with four or more cotyledons. No significant relationships were observed between heterozygosity level and in vitro reactivity, with analyses performed on green embryos only.

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Section: Discussionmentioning

confidence: 99%

Relationship between field performance, family, embryo morphology, and isozyme heterozygosity, and in vitro reactivity in jack pine

Briand¹,

Gauthier²,

Lemay³

et al. 1998

Can. J. For. Res.

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MICROPROPAGATION OF Fagus spp.

Viéitez

San-Jose

Sánchez³

et al. 2003

Micropropagation of Woody Trees and Fruits

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Effect of genotype on rooting of hypocotyls and in vitro-produced shoots of Pinus radiata

Bergmann

Stomp

1994

Plant Cell Tiss Organ Cult

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Significant genotypic variation at both the family and clone-within-family levels was seen for hypocotyl rooting and the rooting of adventitious shoots produced in vitro for Pinus radiata D. Don. High rooting frequencies for hypocotyls were obtained in the absence of exogenous auxin; auxin greatly stimulated the rooting of adventitious shoots. No correlation was seen between the rooting ofhypocotyls and shoots; families whose hypocotyls rooted at high frequencies did not necessarily produce shoots that rooted at high frequency. No correlation was seen between adventitious shoot production and subsequent rooting at either the family or clone level. The lack of a negative correlation indicated that selecting families or clones for high levels of shoot production will not automatically select for low rooting ability, obviating a possible bottleneck for commercial propagation of Pinus radiata. Significant family by replication interaction suggests that rooting protocols could be optimized through manipulations of the rooting environment for each family.

show abstract

Effect of genotype on in vitro adventitious shoot formation in Pinus radiata and correlations between pairs of phenotypic traits during in vitro shoot development

Cited by 9 publications

References 11 publications

Relationship between field performance, family, embryo morphology, and isozyme heterozygosity, and in vitro reactivity in jack pine

Relationship between field performance, family, embryo morphology, and isozyme heterozygosity, and in vitro reactivity in jack pine

MICROPROPAGATION OF Fagus spp.

Effect of genotype on rooting of hypocotyls and in vitro-produced shoots of Pinus radiata

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