Effect of gestational antiandrogen treatment on Dicer1 expression in the porcine fetal gonads

The impact of antiandrogen flutamide on the hypoxia inducible factor 1a and vascular endothelial growth factor A gene and protein expression in the pig placenta during late pregnancy

“…The mRNA expression level of the HIF-1a were quantified in each sample using TaqMan Gene Expression assays (assay ID: Ss03390447_m1; Applied Biosystems), VEGFA (assay ID: Ss033393993) with endogenous control for glyceraldehyde-3-phosphate dehydrogenase (GAPDH; assay ID: Ss03373286_u1) according to the manufacturer's instructions. The amount of each target cDNA was normalized with respect to the GAPDH (DCt value) as previously described [20]. The relative expressions of HIF-1a and VEGFA were presented as 2 −DCt .…”