Effect of globular adiponectin on interleukin-6 and interleukin-8 expression in periodontal ligament and gingival fibroblasts

Park, Hong Gyu; Bak, Eun‐Jung; Kim, Ji Hye; Lee, Yang Sin; Choi, Seong‐Ho; Heon, Jeong; Yoo, Yun‐Jung

doi:10.5051/jpis.2011.41.3.149

Cited by 7 publications

(4 citation statements)

References 36 publications

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“…Although these adipokines could also be detected by real-time PCR, their expressions were very slight and barely modulated. A few studies have reported that HGF express AdipoR1 and AdipoR2, but not adiponectin, which seems to be in contrast to our findings [ 28 , 29 ]. However, in these studies, the expression of adiponectin was analyzed by end-point PCR and immunoblotting, but not real-time PCR, which is a highly sensitive and quantitative assay.…”

Section: Discussioncontrasting

confidence: 99%

Regulation of NAMPT in Human Gingival Fibroblasts and Biopsies

Damanaki

Nokhbehsaim

Eick

et al. 2014

Mediators of Inflammation

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Adipokines, such as nicotinamide phosphoribosyltransferase (NAMPT), are molecules, which are produced in adipose tissue. Recent studies suggest that NAMPT might also be produced in the tooth-supporting tissues, that is, periodontium, which also includes the gingiva. The aim of this study was to examine if and under what conditions NAMPT is produced in gingival fibroblasts and biopsies from healthy and inflamed gingiva. Gingival fibroblasts produced constitutively NAMPT, and this synthesis was significantly increased by interleukin-1β and the oral bacteria P. gingivalis and F. nucleatum. Inhibition of the MEK1/2 and NFκB pathways abrogated the stimulatory effects of F. nucleatum on NAMPT. Furthermore, the expression and protein levels of NAMPT were significantly enhanced in gingival biopsies from patients with periodontitis, a chronic inflammatory infectious disease of the periodontium, as compared to gingiva from periodontally healthy individuals. In summary, the present study provides original evidence that gingival fibroblasts produce NAMPT and that this synthesis is increased under inflammatory and infectious conditions. Local synthesis of NAMPT in the inflamed gingiva may contribute to the enhanced gingival and serum levels of NAMPT, as observed in periodontitis patients. Moreover, local production of NAMPT by gingival fibroblasts may represent a possible mechanism whereby periodontitis may impact on systemic diseases.

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Section: Discussioncontrasting

confidence: 99%

Regulation of NAMPT in Human Gingival Fibroblasts and Biopsies

Damanaki

Nokhbehsaim

Eick

et al. 2014

Mediators of Inflammation

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“…As previously reported, AdipoR1 and AdipoR2 are expressed in PDL cells [65, 71, 72]. Our experiments confirm the expression of both adiponectin receptors in PDL cells.…”

Section: Discussionsupporting

confidence: 92%

“…Another novel finding of our study is the observation that adiponectin is expressed in PDL cells and that it exerts an autostimulatory effect on its expression. As previously reported, AdipoR1 and AdipoR2 are expressed in PDL cells [ 65 , 71 , 72 ]. Our experiments confirm the expression of both adiponectin receptors in PDL cells.…”

Section: Discussionsupporting

confidence: 70%

Beneficial Effects of Adiponectin on Periodontal Ligament Cells under Normal and Regenerative Conditions

Nokhbehsaim

Keser

Nogueira

et al. 2014

Journal of Diabetes Research

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Type 2 diabetes and obesity are increasing worldwide and linked to periodontitis, a chronic disease which is characterized by the irreversible destruction of the tooth-supporting tissues, that is, periodontium. The mechanisms underlying the association of diabetes mellitus and obesity with periodontal destruction and compromised periodontal healing are not well understood, but decreased plasma levels of adiponectin, as found in diabetic and obese individuals, might be a critical mechanistic link. The aim of this in vitro study was to examine the effects of adiponectin on periodontal ligament (PDL) cells under normal and regenerative conditions, and to study the regulation of adiponectin and its receptors in these cells. Adiponectin stimulated significantly the expression of growth factors and extracellular matrix, proliferation, and in vitro wound healing, reduced significantly the constitutive tumor necrosis factor-α expression, and caused a significant upregulation of its own expression. The beneficial actions of enamel matrix derivative on a number of PDL cell functions critical for periodontal regeneration were partially enhanced by adiponectin. The periodontopathogen Porphyromonas gingivalis inhibited the adiponectin expression and stimulated the expression of its receptors. In conclusion, reduced levels of adiponectin, as found in type 2 diabetes and obesity, may compromise periodontal health and healing.

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“…However, a study in human gingival keratinocytes (GK) found no effect of total adiponectin alone on cell proliferation and an inhibitive effect in combination with P. gingivalis LPS (Kraus et al, 2012). Likewise, in separate studies, neither MMP-1, IL-6 nor IL-8 protein expression was affected by total or globular adiponectin in human GK, gingival fibroblasts (GF) or PDL cells (Iwayama et al, 2012;Kraus et al, 2012;Park et al, 2011). Yet, combined stimulation of human GK with P. gingivalis LPS and adiponectin, or of GFs with IL-1β and adiponectin, resulted in decreased IL-6 and IL-8 protein expression compared to IL-1β /LPS stimulation alone (Iwayama et al, 2012;Kraus et al, 2012).…”

Section: Strengths and Limitationsmentioning

confidence: 99%

Adiponectin: Serum-saliva associations and relations with oral and systemic markers of inflammation

Riis

Bryce

et al. 2017

Peptides

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This study addresses gaps in our understanding about the validity and utility of using salivary adiponectin to index serum adiponectin levels. Matched blood and saliva samples were collected on a single occasion from healthy adults (n= 99; age 18-36 years, 53% male). Serum and saliva was assayed for adiponectin and inflammatory cytokines (IL-1β, IL-6, IL-8, TNFα), and saliva was also assayed for markers of blood contamination (transferrin), total protein (salivary flow rate) and matrix metalloproteinase-8 (MMP-8). We examined the extent to which salivary adiponectin was associated with serum adiponectin, and the influence of potential confounders on the serum-saliva correlation, including age, sex, body mass index, and markers of inflammation, oral health, salivary blood contamination, and flow rate. Findings revealed a modest serum-saliva association for adiponectin, and strong positive associations between salivary adiponectin and salivary levels of inflammatory cytokines, MMP-8, transferrin, and total protein. By contrast, salivary adiponectin was not related to serum levels of inflammatory activity. The magnitude of the serum-saliva association was strengthened when controlling for total protein in saliva, blood leakage into oral fluid, salivary inflammatory cytokines, and MMP-8. The pattern of findings extends our understanding of salivary adiponectin and its potential use as an index of circulating adiponectin levels.

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Effect of globular adiponectin on interleukin-6 and interleukin-8 expression in periodontal ligament and gingival fibroblasts

Cited by 7 publications

References 36 publications

Regulation of NAMPT in Human Gingival Fibroblasts and Biopsies

Regulation of NAMPT in Human Gingival Fibroblasts and Biopsies

Beneficial Effects of Adiponectin on Periodontal Ligament Cells under Normal and Regenerative Conditions

Adiponectin: Serum-saliva associations and relations with oral and systemic markers of inflammation

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